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Atrazine molecular imprinting colloidal gold test strip and application

A molecular imprinting, gold standard test paper technology, applied in analytical materials, measuring devices, instruments, etc., can solve problems such as life science, environmental chemistry, food science, pesticide science and other application fields that have not been reported at home and abroad.

Inactive Publication Date: 2013-01-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemically synthesized molecularly imprinted polymers and other polymer materials are used to replace biological antibodies and carrier biological proteins, combined with nano-gold precipitation technology, to carry out research on gold standard test strips for chemically synthesized materials in a complete sense. Environmental chemistry, food science, pesticide science and other application fields have not been reported at home and abroad

Method used

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  • Atrazine molecular imprinting colloidal gold test strip and application
  • Atrazine molecular imprinting colloidal gold test strip and application
  • Atrazine molecular imprinting colloidal gold test strip and application

Examples

Experimental program
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Effect test

preparation example Construction

[0067] 1) Preparation of Atrazine Nano-MIP

[0068]Weigh 0.5 mmol of atrazine (as a template molecule) and place it in a 100 mL glass tube, dissolve it with 50 mL of porogenic solvent (acetonitrile:toluene=3:1 volume ratio), add 1 mmol of functional monomer methacrylic acid (MAA) , mixed and shaken for 1 h, then added 2 mmol of trimethylpropane trimethacrylate (TRIM) as a crosslinking agent and 0.05 g of azobisisobutyronitrile (AIBN) as an initiator and mixed well. The mixture was ultrasonically degassed for 5 minutes, then placed on an ice-water bath for deoxygenation by bubbling nitrogen for 5 minutes, sealed tightly, and placed in a constant temperature oscillator for reaction for 24 hours (60°C, 100rpm). After the reaction stopped, the polymer particles were collected by centrifugation (15000rpm, 15min), and the polymer particles were washed with a washing solution (methanol / acetic acid=9:1) until the supernatant (ie washing solution) could not detect template molecules (H...

Embodiment 1

[0102] Atrazine artificial competitor (ICC-PAMAM G2.0 )Synthesis

[0103]Dendritic flexible organic macromolecules--2.0 generation polyamide-amine dendrimers PAMAM are selected as the original carrier of artificial chemical competition G2.0 . PAMAM G2.0 Molecular formula is C 62 h 128 N 26 o 12 , the molecular weight is 1428. The PAMAM G2.0 For example, it can be purchased from Weihai Chenyuan Silicone New Material Co., Ltd.

[0104] Dissolve 1 mmol of ICC, an incomplete competitor, in 6 mL of DMF, add 0.3 mmol of N-hydroxysuccinimide (NHS), stir for 15 min, then add 0.15 mmol of carbodiimide (DCC) and stir overnight at room temperature; After centrifugation at 4000rpm for 10min, all the supernatant obtained was slowly added dropwise to 6mL dissolved in 0.2mmol PAMAM G2.0 In ultrapure water, magnetically stirred for 4 h. After the reaction is complete, take it out, add an equal volume of glycerol to mix evenly, and store at -20°C; it will be used as the original sto...

Embodiment 2

[0118] Atrazine artificial chemical competitor (ICC-PAMAM G3.0 )Synthesis

[0119] Dendritic flexible organic macromolecules--3.0 generation polyamide-amine dendrimers PAMAM are selected as the carrier of artificial chemical competition G3.0 . PAMAM G3.0 Molecular formula is C 142 h 288 N 58 o 44 , the molecular weight is 3252. PAMAM G3.0 For example, it can be purchased from Weihai Chenyuan Silicone New Material Co., Ltd.

[0120] Dissolve 2 mmol of ICC, an incomplete competitor, in 6 mL of DMF, add 0.3 mmol of N-hydroxysuccinimide (NHS), stir for 15 min, then add 0.15 mmol of carbodiimide (DCC) and stir overnight at room temperature; After centrifugation at 4000rpm for 10min, all the supernatant obtained was slowly added dropwise to 6mL dissolved in 0.1mmol PAMAM G3.0 In ultrapure water, magnetically stirred for 4 h. After the reaction is complete, take it out, add an equal volume of glycerol to mix evenly, and store at -20°C; it will be used as the original stora...

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Abstract

The invention discloses an atrazine molecular imprinting colloidal gold test strip which comprises a base plate, a cellulose nitrate membrane coating complete competition hapten, a water absorption filter paper, a colloidal gold pad, a negative control sample pad and a sample pad to be detected, The atrazine artificial complete competition hapten is coated in a cellulose nitrate membrane coating the complete competition hapten, the atrazine artificial complete competition hapten is a conjugate of the atrazine incomplete competition hapten and a high-molecular carrier; the high-molecular carrier can be any one in the following: polyamide-amine arborization macromolecule with molecular weight M of 1000-50000, polyacrylamide with polymerization degree n of 200-5000, polyamide chloropropylene oxide with polymerization degree n of 100-2000 and glucan with molecular weight M of 1000-50000. The invention also discloses an application of the atrazine molecular imprinting colloidal gold test strip, which is used for high selectivity enrichment, separation or detection of atrazine with trace quantity in the sample to be measured.

Description

technical field [0001] The invention relates to a gold-labeled rapid detection test strip based on nano-molecular imprinting, which is suitable for highly selective enrichment, separation and detection of trace atrazine in complex matrix samples. Background technique [0002] Atrazine (trade name: atrazine, atrazine), chemical name is 2-chloro-4-diethylamino-6-isopropylamino-1,3,5-triazine, is a triazine herbicide It is a selective systemic conduction type pre-emergence and post-emergence herbicide, which is mainly absorbed by roots, and rarely absorbed by stems and leaves. After plant absorption, it can be quickly transmitted to plant meristems and leaves, interfering with photosynthesis The effect is lethal to weeds. It is used to control annual grass weeds and broad-leaved weeds. It also has a certain inhibitory effect on perennial weeds. It is still widely used in the cultivation of corn and grains, and it pollutes groundwater more seriously. It has been designated as o...

Claims

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Application Information

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IPC IPC(8): G01N33/558
Inventor 桂文君谢蓉郭逸蓉刘菲菲朱国念
Owner ZHEJIANG UNIV
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