Method for preparing rapamycin/magnetic carboxymethyl chitosan nano drug-loaded microspheres

A technology of carboxymethyl chitosan and rapamycin is applied in the application field of biofunctional materials in the field of biomedicine, which can solve the problem of inability to solve normal tissue damage, failure to achieve optimal therapeutic effects, and drug-loaded microspheres. problems such as poor targeting, to achieve the effect of strengthening the effect of targeted therapy, low toxicity administration, and high drug loading
CN102961345AInactive Publication Date: 2013-03-13GUILIN UNIV OF ELECTRONIC TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUILIN UNIV OF ELECTRONIC TECH
Publication Date
2013-03-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for preparing rapamycin / magnetic carboxymethyl chitosan nano drug-loaded microspheres. According to the method, synthesized Fe3O4 nanoparticles are added to liquid paraffin oil, the liquid paraffin oil is then mixed with carboxymethyl chitosan liquid, a crosslinking agent is added, nanospheres are collected through magnetic separation, and magnetic carboxymethyl chitosan nanospheres are obtained through washing and drying; aqueous dispersion liquid is prepared by using the obtained magnetic carboxymethyl chitosan nanospheres, rapamycin is dissolved in acetonitrile, and stirring is carried out so as to mix the rapamycin and the acetonitrile; and magnetic separation is carried out, a lower layer of precipitate is washed by using ultrapure water, and the rapamycin / magnetic carboxymethyl chitosan nano drug-loaded microspheres are obtained through freeze-drying and crushing. The nano drug-loaded microspheres prepared by the method have the characteristics of strong targeting, high drug loading capacity, good slow release performance, small particle size, low drug toxic and side effect and the like, and the tumor cell killing rate of rapamycin drugs can be remarkably increased; and the method is simple in process, mild in preparation conditions and easy in scale production.
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Description

technical field

[0001] The invention relates to the application of a biofunctional material in the field of biomedicine, in particular to a method for preparing nano drug microspheres by using magnetic carboxymethyl chitosan to fix rapamycin. Background technique

[0002] Rapamycin (RAPA) is a new macrolide immunosuppressant isolated from the fermentation broth of Streptomyces hygroscopicus. It not only has anti-transplant immune rejection, but also has a strong inhibitory effect on prostate cancer, kidney cancer, breast cancer, colon cancer and liver cancer. RAPA can regulate gene expression by inhibiting the protein kinase catalytic activity of mammalian target of rapamycin (mTOR) and the phosphoinositide-3 kinase (PI3 K) / Akt signal transduction pathway , cell growth and proliferation, by blocking different cytokine receptor signal transduction, to block the progress of T lymphocytes and other cells from G1 phase to S phase, thereby exerting its immunosuppressive effect. ...

Claims

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