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Method for detecting quality of cordia dichotoma

A quality inspection method and technology of ragwood, which are applied in measurement devices, color/spectral property measurement, instruments, etc., can solve the problems of little research on ragwood and no detection method for quality control established.

Active Publication Date: 2013-05-01
XINJIANG CICONHABO UYGUR MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are few related researches on ragbuk fruit, and no detection method for quality control has been established.

Method used

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  • Method for detecting quality of cordia dichotoma
  • Method for detecting quality of cordia dichotoma
  • Method for detecting quality of cordia dichotoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The qualitative analysis of rosmarinic acid in the saplings by thin layer chromatography is as follows:

[0026] Add the reference substance rosmarinic acid to methanol to make a control solution with a concentration of 1.4mg / mL, add 5g of ragberry medicinal powder to 50mL of 70% ethanol, ultrasound for 30min (power: 80, temperature: 30℃), filter After that, the filtrate is concentrated and evaporated to dryness and dissolved in methanol as the test solution of the original medicinal material. The specific process of thin-layer chromatography is: on the same silica gel G thin-layer plate, use ethyl acetate-formic acid-acetic acid-water as the developing solvent at a volume ratio of 15:1:1:1.5, and rosmarinic acid- The methanol solution is at the 1, 4 positions, and the ragberry medicinal material-methanol solution is developed at the 2, 3 positions. After drying, spray color developer 1.5% AlCl 3 After heating and drying the ethanol solution in a thin-layer color heater a...

Embodiment 2

[0029] The qualitative analysis of rosmarinic acid in the saplings by high performance liquid chromatography and mass spectrometry is as follows:

[0030] Chromatographic conditions: high performance liquid chromatograph, column: Agilent column (4.6 x 250mm, 5??m); mobile phase: A is methanol, B is dilute phosphoric acid; detection wavelength is 273nm; column temperature is 30°C; flow rate It is 1mL / min. Using gradient elution, the volume percentage of B in the mobile phase changes with time as follows: 0-6min, linearly decreasing from 70% to 55%; 6-20min, linearly increasing from 55% to 45%.

[0031] Mass spectrometry conditions: ionization method is electrospray ionization (ESI) method; scanning method is negative ion mode; dryer temperature: 350℃, dryer pressure 55psi, dryer flow rate 12L / min, capillary voltage 3500V; rosmarinic acid The scanning range of the primary mass spectrum is m / z350-370; the precursor ion of the secondary mass spectrum is m / s359, the fragmentation volta...

Embodiment 3

[0036] The Folin-Ciocalteu method is used to determine the content of total polyphenols in Oryza sylvestris. The specific steps are as follows:

[0037] Preparation of standard solution: accurately weigh 10.0005g of gallic acid standard, place it in a 100ml volumetric flask, add an appropriate amount of distilled water, dissolve by ultrasound, let cool, dilute with distilled water to the mark, shake well to get 0.1mg / ml gallic acid standard solution.

[0038] Standard curve drawing: accurately draw 4mL, 5mL, 6mL, 7mL, 8mL of gallic acid standard solution to a 10mL volumetric flask, respectively take 2mL and place in a test tube with a lid, add 4mL of distilled water, shake well , First add 0.5ml of Folin-Ciocalteu reagent (Folin-phenol reagent) to each test tube, then add 20% Na 2 CO 3 Set the volume to 10 mL, shake well, cap and tighten, cool quickly after 10 minutes in a 75°C water bath, and measure the absorbance at 765 nm with a spectrophotometer. Then draw a standard curve wi...

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Abstract

The invention discloses a method for detecting the quality of cordial dichotoma. The method comprises steps of qualitative identification of rosmarinic acid, qualitative analysis of the rosemarinic acid, content measurement of total polyphenol, content measurement of total triterpenic acid and quantitative analysis of the rosmarinic acid. The method has the benefit that the qualitative analysis and the quantitative analysis of the cordial dichotoma are carried out one by one, is quick and accurate, achieves monitoring to main ingredients in the cordia dichotoma via the qualitative analysis and the quantitative analysis of the rosemary acid, establishes the quality standard of the cordia dichotoma by coordinating with the content measurement of the total polyphenol and the total triterpenic acid, provides the effective evaluation of quality of the cordia dichotoma with a basis, and meanwhile facilitates quality control during production process of enterprises.

Description

technical field [0001] The invention relates to a method for detecting the quality of medicines, in particular to a method for detecting the quality of rags. Background technique [0002] Cordia dichotoma Forst.f. is the dried and mature fruit of Cordia dichotoma Forst.f., which has the functions of generating dampness and heat, clearing abnormal mucous temperament, regulating bile balance, moistening lung and relieving cough, voiceless sound and quenching thirst. It has the effect of promoting diuresis, broadening the chest and resolving phlegm. It is used for dry cold or black gallbladder diseases. It can treat persistent cough, strong bile, unpleasant phlegm, aphonia and thirst, dry throat and itchy throat, difficulty urinating, and defecation. Not smooth. However, at present, there are few related researches on the fruit of Ragbukia, and no detection method for quality control has been established. Contents of the invention [0003] The object of the present inventio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/02G01N21/31
Inventor 田树革季志红哈木拉提·吾甫尔陈金成
Owner XINJIANG CICONHABO UYGUR MEDICINE
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