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Detection reagent for aspartate aminotransferase

A technology of aspartic acid amino group and detection reagent, which is applied in the field of medical inspection, can solve the problems of insufficient automatic biochemical inspection equipment and lack of inspection professionals, and achieve the effects of good sensitivity, low price, and easy operation

Active Publication Date: 2015-07-08
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] With the "Eleventh Five-Year" plan for the purchase and strengthening of basic equipment for tertiary and secondary hospitals, hospitals above the middle level have good hardware and software facilities, but grassroots medical institutions, especially health centers, clinics and other units are not fully automatic. Biochemical testing equipment and facilities are generally insufficient, and there is not a sufficient number of testing professionals. Therefore, the establishment of a POCT analysis system that is easy to operate, low in price, and real-time reporting is of great significance to the role of grassroots hospitals in disease prevention and diagnosis and treatment.

Method used

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  • Detection reagent for aspartate aminotransferase
  • Detection reagent for aspartate aminotransferase
  • Detection reagent for aspartate aminotransferase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Diluent:

[0047] Tris hydroxymethyl amino buffer (pH7.0) 0.1mol / L

[0048] TWEEN80 5%

[0049] Proclin300 0.1%

[0050] Bilirubin oxidase 1KU / L

[0051] Vitamin C oxidase 1KU / L

[0052] Reagent:

[0053] Tris hydroxymethyl amino buffer (pH6.0) 0.1mol / L

[0054] Aspartic acid 5mol / L

[0055] α-ketoglutarate 0.5mol / L

[0056] Oxaloacetate decarboxylase 20KU / L

[0057] Phosphoric acid 10mol / L

[0058] Pyruvate oxidase 30KU / L

[0059] Thiamine pyrophosphate 40mol / L

[0060] Magnesium chloride 20mol / L

[0061] Peroxidase 20KU / L

[0062] 4-Aminoantipyrine 1mol / L

[0063] Sodium N-ethyl-N-(3-sulfopropyl)-3-methoxyaniline 2mol / L

[0064] Potassium sorbate 0.1g / L

[0065] Bovine serum albumin 5g / L

Embodiment 2

[0067] Diluent:

[0068] Tris hydroxymethyl amino buffer (pH7.5) 0.5mol / L

[0069] TRITONX-100 5%

[0070] Methylparaben 1%

[0071] Potassium ferrocyanide 1mol / L

[0072] Vitamin C oxidase 2KU / L

[0073] Reagent:

[0074] 3-(N-morpholine)sodium ethanesulfonate buffer solution (pH7.2) 0.5mol / L

[0075] Aspartic acid 20mol / L

[0076] α-ketoglutarate 2mol / L

[0077] Oxaloacetate decarboxylase 50KU / L

[0078] Phosphoric acid 20mol / L

[0079] Pyruvate oxidase 100KU / L

[0080] Thiamine pyrophosphate 20mol / L

[0081] Magnesium chloride 20mol / L

[0082] Peroxidase 10KU / L

[0083] 4-Aminoantipyrine 0.5mol / L

[0084] N-ethyl-N-(2-hydroxy-3-propanesulfonyl) m-toluidine 2mol / L

[0085] Proclin300 1g / L

[0086] Trehalose 10g / L

Embodiment 3

[0088] Diluent:

[0089] 3-Morpholinepropanesulfonic acid buffer (pH6.5) 1.0mol / L

[0090] SPAN20 10%

[0091] Sodium Benzoate 1%

[0092] Potassium ferricyanide 10mol / L

[0093] Vitamin C oxidase 10KU / L

[0094] Reagent:

[0095] Piperazine-N,N-bis(2-hydroxyethanesulfonic acid) buffer solution (pH8.0) 1mol / L

[0096] Aspartic acid 100mol / L

[0097] α-ketoglutarate 10mol / L

[0098] Oxaloacetate decarboxylase 200KU / L

[0099] Phosphoric acid 50mol / L

[0100] Pyruvate oxidase 200KU / L

[0101] Thiamine pyrophosphate 80mol / L

[0102] Magnesium chloride 30mol / L

[0103] Peroxidase 100KU / L

[0104] 4-Aminoantipyrine 5mol / L

[0105] N-ethyl-N-(3-sulfopropyl)-3-methoxyaniline sodium salt 5mol / L

[0106] Proclin300 1g / L

[0107] Fatty alcohol polyoxyethylene ether 10g / L

[0108] The performance of the reagent obtained in Example 1 of the present invention is described below in conjunction with the table.

[0109] 1. Precision

[0110] Table 1. Precision evaluation res...

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Abstract

The invention discloses a detection reagent for aspartate aminotransferase. The detection reagent comprises a diluent and a reaction reagent. The diluent comprises a buffer, a surfactant, an antiseptic, a bilirubin-interference removing reagent, and an ascorbic oxidase. The reaction reagent comprises a buffer, aspartic acid, alpha-ketoglutaric acid, oxaloacetic decarboxylase, phosphoric acid, pyruvate oxidase, thiamine pyrophosphate, magnesium chloride, peroxidase, 4-aminoantipyrene, a chromogen, an antiseptic, and a stabilizer. The detection reagent has advantages of good sensitivity, accuracy, precision and linearity, and can completely meet the requirements of clinical examination.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to an aspartate aminotransferase detection reagent for a POCT analyzer. Background technique [0002] Liver disease is a common and extremely harmful disease. Its timely diagnosis and treatment play an important role in the prevention, diagnosis and treatment of the disease. Aspartate aminotransferase (AST) exists widely in various tissues in the body. The heart activity is the highest, and the content in normal human serum is very small. A high AST indicates liver cell damage, which is generally seen in various liver and gallbladder diseases such as hepatitis B, cirrhosis, fatty liver, and alcoholic liver. At present, AST generally adopts various large-scale automatic biochemical analyzers for detection, but due to the high price of large-scale automatic biochemical analyzer equipment and complicated operation, operators need to have relevant professional knowledge and rec...

Claims

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Application Information

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IPC IPC(8): C12Q1/527C12Q1/52C12Q1/30C12Q1/28C12Q1/26G01N21/31
Inventor 邹炳德邹继华张桂春沃燕波
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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