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Enteromorpha se-polysaccharide with biological activity as well as preparation method and application of enteromorpha se-polysaccharide

A bioactive, selenium polysaccharide technology, applied in the preparation of selenium polysaccharides from Enteromorpha selenium polysaccharides, the field of selenium polysaccharides from Enteromorpha selenium, can solve the problems of incompletely clear chemical structure of selenium polysaccharides, complex structure of selenized polysaccharides, limited types of selenium polysaccharides, etc. , to achieve the optimization of physiological and pharmacological functions, good social and economic benefits, and the effect of facilitating absorption and utilization

Inactive Publication Date: 2013-10-02
QINGDAO VIP PET HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the types of selenium polysaccharides found or artificially synthesized in China are still very limited, mainly including garlic selenium polysaccharides, astragalus selenium polysaccharides, ganoderma lucidum selenium polysaccharides, wolfberry selenium polysaccharides, etc. The research on enteromorpha selenium polysaccharides has not been reported, and the selenized polysaccharides The structure is complex, and the determination of the chemical structure of selenium polysaccharides and the mechanism of action in vivo are not yet fully understood

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Extraction of Enteromorpha active crude polysaccharide:

[0025] (1) Degreasing and dealcohol-soluble substances of Enteromorpha: pulverize Enteromorpha, pass through a sieve with an aperture of 0.4 mm, then degrease with ether, and obtain a degreased sample after drying, then reflux 3 times with 95% ethanol, and dry the alcohol-insoluble matter ;

[0026] (2) Enzymatic deproteinization:

[0027] Add an appropriate amount of distilled water to the prepared alcohol-insoluble matter, adjust the pH to 6.5, add papain equivalent to 1.5% of the alcohol-insoluble matter mass, incubate in a constant temperature magnetic stirrer at 60°C for 2 hours, concentrate in vacuo, and add ethanol to the concentrated solution to make The volume fraction is 80%, and the obtained precipitate is washed with absolute ethanol, acetone and ether in sequence, and dried to obtain the primary product of crude polysaccharide;

[0028] (3) Activated carbon decolorization:

[0029] The deproteiniz...

Embodiment 2

[0031] Synthesis of enteromorpha selenium polysaccharide:

[0032] Put the prepared crude polysaccharide of Enteromorpha enteromorpha into a flask, add nitric acid solution, heat and stir to dissolve; then add sodium selenite, heat and stir, react for a certain period of time, cool, filter, and centrifuge to remove the precipitate, and the obtained solution is dialyzed with convective water, Small molecules were removed, distilled under reduced pressure to a thick paste, precipitated with absolute ethanol, frozen overnight, centrifuged, the obtained precipitate was washed with absolute ethanol, and vacuum freeze-dried to obtain the selenized crude polysaccharide of Enteromorpha enteromorpha.

Embodiment 3

[0034] Purification of selenium polysaccharides from Enteromorpha enteromorpha:

[0035] Put the G-10 gel in 1% sodium chloride solution, take it out after fully soaking for two days, and then wash 4 column volumes with 1% sodium chloride solution until the effluent is free of turbidity; use Sephadex gel column chromatography to separate the The selenium polysaccharide part of the moss was dialyzed to remove small molecular substances, and the deionized selenium polysaccharide of Enteromorpha was eluted with 1% sodium chloride solution, the eluate was collected and concentrated, alcohol precipitation, suction filtration, and freeze-drying to obtain pure enteromorpha selenium polysaccharide.

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PUM

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Abstract

The invention discloses an enteromorpha se-polysaccharide with a biological activity, which is characterized in that the enteromorpha se-polysaccharide is heteropolysaccharide consisting of rhamnose, xylose, mannose, glucose and selenide groups. The invention further discloses a preparation method and an application of enteromorpha se-polysaccharide. According to the invention, an enteromorpha se-polysaccharide pure product with high added values and biological activity is prepared, selenium-containing content of polysaccharide is improved, so that physiological and pharmacological functions of selenium and enteromorpha se-polysaccharide are optimized, the activity is higher than selenium and enteromorpha se-polysaccharide, enteromorpha se-polysaccharide is beneficial to absorption and utilization of a human body, waste is changed into things of value, and enteromorpha se-polysaccharide has good social benefits, economic benefits and a wide application prospect.

Description

technical field [0001] The invention relates to a bioactive enteromorpha selenium polysaccharide, in particular to a preparation method and application of the enteromorpha selenium polysaccharide, belonging to the technical field of biological preparations. Background technique [0002] Enteromorpha is a large green alga belonging to Chlorophyta, Chlorophyceae, Ulvales, Ulvaceae, and Enteromorpha genus. Enteromorpha contains more water-soluble sulfated polysaccharides. Enteromorpha polysaccharide is one of the main active ingredients contained in Enteromorpha, which has biological activities such as lowering blood fat, anti-fatigue, antibacterial, antiviral and enhancing immunity. Among the wild algae in our country, Enteromorpha is rich in resources, and its natural reproductive ability is extremely strong. When it breaks out, it will cause natural disasters. [0003] Due to global climate change, water eutrophication and other reasons, frequent outbreaks of green tides of...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P17/16A61P35/00A23L1/09A23L33/125A23L33/16
Inventor 吕海涛周前徐鲁斌王清吉马传利邓锐高玉杰
Owner QINGDAO VIP PET HEALTH PROD
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