Preparation method and application of dracocephalum heterophyllum benth volatile oil

A technology of Chrysanthemum heterophylla and volatile oil, which is applied in the field of traditional Chinese medicine and anti-respiratory virus drugs, can solve the problems of no effective preventive vaccine, inability to prevent re-infection, weak immunity, etc., and achieve good medicinal prospects, rich raw materials, and inhibit The effect of viral gene replication

Active Publication Date: 2013-12-11
兰州诺然肠道健康精准营养研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

RSV proliferates in the epithelial cells of the respiratory tract, and bronchial necrosis adheres to mucus and fibrin, which can easily lead to airway obstruction, an

Method used

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  • Preparation method and application of dracocephalum heterophyllum benth volatile oil
  • Preparation method and application of dracocephalum heterophyllum benth volatile oil
  • Preparation method and application of dracocephalum heterophyllum benth volatile oil

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1 The preparation of the volatile oil and extract of Qinglan heterophylla

[0025] (1) Preparation of volatile oil

[0026] Take an appropriate amount of whole herb (broken into about 1 cm long) and put it into a 2000ml round-bottomed flask, steam it, distill it with water steam for 1.5 hours, and collect the distillate; use anhydrous ether to extract the distillate several times in small amounts, and collect the organic phase; Add anhydrous Na 2 SO 4 Dry the organic phase; distill the organic phase under normal pressure in the presence of zeolite, recover the distillate ether, collect the yellow oil in the flask, and obtain the volatile oil of Cynophyllum indigo; store the volatile oil at -20°C, and sterilize it by ultraviolet irradiation for 30 minutes before use .

[0027] (2) Preparation of the fractionated extract of Qinglan isophyllum

[0028] Soak the green orchid in alcohol for 7 days, during which the active ingredients are extracted by frequent ...

Embodiment 2

[0029] Example 2 Chromography analysis of volatile oil

[0030]The components of the volatile oil of Qinglan isophyllum were analyzed by gas chromatography-mass spectrometry (Trace DSQ GC-MS instrument, American Thermo Electric Company). The gas chromatography conditions are: column DB-5ms; column length: 30m; liquid film thickness: 0.25μm; inner diameter: 0.25mm; carrier gas: He; flow rate: 1.0mL / min; 10°C / min), constant temperature for 20min; injection volume 1μL. Mass spectrometry conditions: split mode injection, split ratio 30:1; inlet temperature: 250°C; auxiliary line temperature: 280°C; ion source temperature: 250°C; ionization mode: EI; electron energy: 70eV; electron multiplier tube voltage : 1256kV; acquisition method: scanning; scanning mass range: 40~600amu.

[0031] The volatile oil was analyzed by gas-mass spectrometry, and the retention time (in sequence), name, molecular formula, relative percentage and coincidence of various components are shown in Table 1....

Embodiment 3

[0035] Example 3 In vitro antiviral effect of volatile oil and extract

[0036] (1) Cytotoxicity of the volatile oil and fractionated extracts of Cymbidium heterophylla

[0037] 1 Viruses and cell lines

[0038] Adenovirus (adenovirus, ADV3), influenza A virus H1N1, MDCK (dog kidney cells), Hep-2 (human laryngeal cancer) cells were provided by Gansu Provincial Center for Disease Control.

[0039] 2 animals

[0040] Kunming mice, half male and half male, weighing 18-22g, Medical Animal Experiment Center of Lanzhou University

[0041] 3 drugs

[0042] Use 1% DMSO as solvent to prepare the fractionated extract of the volatile oil of Indigo isophyllum, and acyclovir powder injection (Wuhan Pusheng Pharmaceutical Co., Ltd.) as a positive control

[0043] Cells were cultured, and the volatile oil and extract were serially diluted to 5 dosage concentrations (4mg / ml, 2mg / ml, 1mg / ml, 0.5mg / ml, 0.25mg / ml) with cell maintenance solution, and added to the cells already In a 96-well p...

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Abstract

The invention relates to a preparation method and application of dracocephalum heterophyllum benth volatile oil. Compared with existing antiviral synthetic drugs, the dracocephalum heterophyllum benth volatile oil has low toxicity and strong drug action and is safe and reliable. Moreover, the dracocephalum heterophyllum benth raw material is rich and cheap, is simple in extraction process and low in preparation cost and can be prepared into various dosage forms.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to an anti-respiratory tract virus drug, in particular to the application of the volatile oil of Cymbidium heterophylla in the preparation of anti-respiratory tract virus drug. Background technique [0002] Tibetan medicine Dracocephalum heterophyllum Benth is the dry aboveground part of Dracocephalum heterophyllum Benth, a perennial herb, also known as Baihuazhizihua, Baihua Prunella, Jiziqingbao (Tibetan medicine name), Xinjiang Uighur name Marzan Jusi, Zupar. The aerial part of Chrysanthemum variegata has the effect of clearing and purging liver heat, and it is a traditional medicine used by Uighurs and Tibetans to treat cough, asthma and stomach problems. It is mainly used to treat various diseases such as icteric fever, liver heat, febrile headache, cloudy eyes, oral ulcers, bronchitis, and high blood pressure. It is a species included in the standards issued by the ...

Claims

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Application Information

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IPC IPC(8): C11B9/02A61K36/53A61P31/12A61P11/00
Inventor 张春江
Owner 兰州诺然肠道健康精准营养研究所
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