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Bio-control streptomyce for straw degradation and application thereof

A technology for biocontrol of Streptomyces and straw, applied in the field of soil remediation, can solve problems such as affecting crop growth, difficulty in utilization, and poor soil, and achieve the effects of good application potential, strong tolerance, and increasing soil organic matter content

Inactive Publication Date: 2014-02-05
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If used improperly, it will not only cause environmental pollution but also waste resources. Therefore, straw is the most abundant and also the most difficult to use carbon pool in nature; at the same time, the accumulation of nitrate is the main cause of secondary salinization of facility soil. Not only does nitrate nitrogen directly affect crop growth, but when nitrate nitrogen exceeds the growth needs of soil microorganisms and plants, it changes direction and leads to soil "carbon impoverishment"

Method used

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  • Bio-control streptomyce for straw degradation and application thereof
  • Bio-control streptomyce for straw degradation and application thereof
  • Bio-control streptomyce for straw degradation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of Streptomyces JSD-1 Spore Suspension

[0033] Take the slant of Streptomyces grown on Gaoshi No. 1 solid medium for 3 days, and add 9 mL of sterile water. Gently scrape off the spores with a sterile loop to keep the spores in suspension. Pour the crude spore suspension into a 50 mL centrifuge tube and shake as much as possible on a shaker for about 1 min. Filter the suspension with a test tube equipped with absorbent cotton, pour the green leaves into a centrifuge tube, centrifuge at 3000rpm for 5min to precipitate the spores, and pour out an appropriate amount of supernatant to make the concentration of the spore suspension after mixing 2×10 9 cfu / mL.

[0034] The components of the Gaoshi No. 1 medium are: soluble starch 20.0g, KNO 3 1.0g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl0.5g, FeSO 4 ·7H 2 O0.01g, 1L of ultrapure water, adjust the pH to 7.2-7.4. When preparing, first use a small amount of cold water to make the starch into a paste, pour ...

Embodiment 2

[0036] Streptomyces JSD-1 on fermentation broth (KNO 3 =1g / L) Nitrate nitrogen degradation test

[0037] Take 0.5mL Streptomyces spore suspension and insert 50mL KNO 3The concentration is 1g / L in the fermentation broth, to simulate the salt concentration (content ≥ 1g / L) environment in the secondary salinized soil of mild facilities. Cultured at 30°C and 150rpm for 5 days, samples were taken every 24 hours and diluted 30 times, and the content of nitrate nitrogen in the fermentation broth was measured with an AA3 flow injector. The content of nitrate nitrogen in the fermentation broth was determined as figure 1 As shown, the concentration of nitrate nitrogen in the fermentation broth decreased rapidly on the 1st day; by the 3rd day, the nitrate nitrogen content in the fermentation broth had dropped to 14.3 mg / L, which was nearly 90% lower than the initial concentration; by the 4th day, The content of nitrate nitrogen in the fermentation broth dropped to a very low level, th...

Embodiment 3

[0040] Streptomyces JSD-1 on fermentation broth (KNO 3 =5g / L) Nitrate nitrogen degradation test

[0041] Take 0.5mL Streptomyces spore suspension into 50mL KNO 3 Concentration is 5g / L in the fermented liquid, to simulate the salinity concentration (content ≥ 5g / L) environment in secondary salinized soil of moderate facility. Cultured at 30°C and 150rpm for 5 days, sampled every 24 hours and diluted 200 times, then used AA3 flow injector to measure the content of nitrate nitrogen in the fermentation broth. The content of nitrate nitrogen in the fermentation broth was determined as figure 2 As shown, the concentration of nitrate nitrogen in the fermentation broth began to decrease on the first day, and there was no obvious process of adapting to the environment; the degradation rate of nitrate nitrogen was relatively stable throughout the 5-day fermentation period. at KNO 3 In the fermentation broth with a content of 5g / L, the degradation rate of nitrate nitrogen by Strepto...

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Abstract

The invention discloses bio-control streptomyce for straw degradation and an application thereof in the technical field of soil restoration. A method for preparing the streptomyce comprises strain preservation and strain activation, wherein the strain preservation uses a low-temperature freezing preservation method at minus 20 DEG C to reduce the metabolism velocity of microbial spores at a low temperature to achieve the purpose of strain preservation; the strain activation uses a method of inoculating strains in a freezing preservation state into a YEME (yeast extract-malt extract) liquid culture medium, and breeding the strains when the strains are gradually adaptive to the culture environment, so as to obtain cultures with enough quantity and vigour. In addition, the invention relates to an application for the streptomyce JSD-1 in bio-control. According to the streptomyce and the application disclosed by the invention, in-situ degradation for carbon which is difficult to use in straws and excessive nitrogen in facility secondary salinized soil is realized by virtue of the undisclosed nitrate nitrogen degradation transformation capacity as well as the characteristics such as the very strong tolerance to nitrate with a high temperature (for example, 70 DEG C) and a high concentration (for example, 3%), the great inhibition effect on the microbes of plant pathogens (for example, botrytis cinerea and rice bakanae disease), and the like of the known JSD-1 strains, and the bio-control streptomyce has a great application potentiality in developments for high temperature compost external bacteria agents and bio-fertilizers.

Description

technical field [0001] The invention relates to a method in the technical field of soil remediation, in particular to a straw-degrading Streptomyces strain with high-efficiency nitrate-nitrogen conversion capability and its application. Background technique [0002] In facility cultivation agriculture, due to the special water transport mode under cultivation conditions such as greenhouses and greenhouses, the leaching of the soil by natural rainfall outside is artificially cut off, and the temperature, humidity, ventilation conditions and water and fertilizer management are quite different from those in open field cultivation. In addition, protected cultivation has been in a state of high intensification, high multiple cropping index, and high fertilizer application for a long time, and a large amount of salt ions that have not been absorbed by plants will be deposited in the soil. [0003] Excessive accumulation of soil salt will cause the osmotic potential of crop rhizosp...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
Inventor 周培冯海玮支月娥罗艳青毛亮孙玉静唐冬卫星时唯伟
Owner SHANGHAI JIAO TONG UNIV
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