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Microorganisms and their application in the preparation of biological fertilizers, biological fertilizers and their applications

A bio-fertilizer and microbial technology, applied in the field of bio-fertilizer and its application, can solve the problems of fertility decline, acidification, and failure to improve soil physical and chemical properties, and achieve the effect of improving physical and chemical properties, solving crop yield, and increasing crop yield

Inactive Publication Date: 2015-11-18
SHANDONG PUFANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the microorganisms contained in the above-mentioned biological fertilizers are traditional nitrogen-fixing, phosphorus-dissolving and potassium-dissolving bacteria. Although they can improve the utilization rate of nutrients in the soil by crops, they do not have the effect of improving the physical and chemical properties of the soil.
However, due to the extensive use of chemical fertilizers, soil physical and chemical properties have deteriorated, such as soil acidification, compaction, and fertility decline, making it no longer suitable for the growth needs of crops. Just considering how to improve the utilization rate of nutrients in the soil cannot fundamentally solve the problem. The problem of increasing crop yield and making agricultural production sustainable

Method used

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  • Microorganisms and their application in the preparation of biological fertilizers, biological fertilizers and their applications
  • Microorganisms and their application in the preparation of biological fertilizers, biological fertilizers and their applications
  • Microorganisms and their application in the preparation of biological fertilizers, biological fertilizers and their applications

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1, the screening of this microorganism

[0030] 1. Soil sample collection

[0031] Soil samples were collected from Shouguang City, Shandong Province. After selecting the sampling site, shovel off 2-3 cm of the topsoil layer, take about 10 g of soil at a depth of 3-10 cm, put it into a sterilized kraft paper bag, seal the bag, and record the sampling site, environment and date. Soil samples should be separated in time after collection, and any samples that cannot be separated immediately should be stored in low temperature and dry conditions to minimize the change of bacterial phase.

[0032] 2. Separation and purification: gradient dilution plate streaking method

[0033] Pour plate: Heat and melt the sterilized solid medium, pour it into the petri dish on the operating table in the sterile room, pour 15-20 ml into each petri dish, and operate by the flame of the alcohol lamp. At this time, care should be taken not to Touch the mouth of the Erlenmeyer fla...

Embodiment 2

[0045] Embodiment 2, the preparation of this microbial spore suspension

[0046] Specifically prepare as follows:

[0047] Transfer the refrigerated microbial strains to the PDA medium plate (200g potato, 20g sucrose, 18g agar, 1000mL distilled water) for 3 generations; then use an inoculation loop to pick the edge hyphae of the 3rd generation colony and transfer to PDB In the liquid medium (200g of potatoes, 20g of sucrose, 1000mL of distilled water), culture at 25°C to 30°C, the optimum temperature is 28°C, 120rpm, for 10 days to obtain the fermentation liquid of this microorganism. Filter the obtained fermented liquid through a 100 μm sieve to obtain a spore suspension, and the live spore concentration is 3.0-5.0×10 10 cfu / ml.

Embodiment 3

[0048] Embodiment 3, the preparation method of decomposed organic matter

[0049] In the embodiment of the present invention, the decomposed organic matter can be prepared according to the following method:

[0050] Wet 1000kg of dry-based raw materials composed of one or more organic wastes such as crop straw, leaves, weeds, sawdust, distiller's grains, furfural slag, etc. with water, so that the water content of the raw materials reaches 50-60% When mixing, mix 5kg of enzyme powder with the above-mentioned raw materials with a water content of 50-60%, pile them into a strip-shaped pile with a width of 2 meters, a height of 1 meter, and unlimited length, and cover it with plastic cloth. After 24 hours, make the temperature in the strip pile reach 60-70°C, after 48 hours, make the temperature in the strip pile reach 80-90°C, after 6-7 days, make the temperature in the strip pile drop naturally, 9-10 Days later, the fermentation was finished to obtain decomposed organic matter...

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Abstract

The invention provides a microbe, an application of the microbe in bio-fertilizer preparation, a bio-fertilizer and an application of the bio-fertilizer. Compared with the existing microbe for the bio-fertilizer and the existing bio-fertilizer in the prior art, the microbe provided by the invention refers to trichoderma viride, can produce abundant hypha, efficiently express the high-activity special enzyme system such as beta-1,4-glucosan hydrolase, chitinase, cellulase, protease to generate volatile or non-volatile active substances capable of inhibiting pathogenic bacteria growth, so that the microbe can be used for preparation of the bio-fertilizer. When the bio-fertilizer is used for promoting crop growth, the bio-fertilizer can preferably improve the soil physicochemical property, so as to improve the crop yield and facilitate sustained development of the agricultural production fundamentally.

Description

technical field [0001] The invention relates to the field of biological fertilizers, in particular to microorganisms and their application in preparing biological fertilizers, biological fertilizers and their applications. Background technique [0002] In agricultural production, in order to increase production, chemical fertilizers are used in large quantities, resulting in various environmental pollution problems. Biological fertilizer is a kind of green and efficient fertilizer because it will not pollute the environment. It has been rapidly developed and widely used in crop cultivation. [0003] In related technologies, the research on biological fertilizers mainly focuses on how to improve the utilization rate of nutrients in soil. For example, research on rhizobia agents for symbiotic nitrogen fixation, research on autogenous and combined nitrogen-fixing bacteria, various bacillus, photosynthetic bacteria, and cellulolytic bacteria, lactic acid bacteria, yeast and oth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05F11/08C12R1/885
Inventor 杨文慧
Owner SHANDONG PUFANG BIOTECH