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A strain of Saccharomyces cerevisiae and its application in the production of ethanol by fermentation

A technology of Saccharomyces cerevisiae and ethanol, applied in the direction of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of low ethanol yield and high production cost of ethanol produced by fermentation, achieve high economic value, increase fermentation yield and improve tolerance Effect

Inactive Publication Date: 2016-01-13
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of low ethanol production in the current fermentation ethanol production, no strains with high ethanol production, and high production cost of ethanol production by fermentation, the present invention provides a strain of Saccharomyces cerevisiae SC-X234 and its application in the production of ethanol by fermentation. The change improves the tolerance of Saccharomyces cerevisiae to alcohol in fermentation, while ensuring the ethanol production at the level of 15.75%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The screening method of embodiment 1 Saccharomyces cerevisiae SC-X234

[0032] The starting strain, Saccharomyces cerevisiae SC-100, was purchased from Angel Yeast Co., Ltd. Angel yeast dry powder for easy labeling, and the laboratory named it SC-100.

[0033] Screening Saccharomyces cerevisiae SC-X234 with SC-100 as the starting strain, the specific steps are as follows:

[0034] a) Preparation of single spore suspension: make spore suspension from Saccharomyces cerevisiae at a concentration of 1×10 6individual / mL.

[0035] b) Low-energy ion beam-mediated mutagenesis of the spt15 gene: Take 100 μL of spore suspension and 50 μL of the plasmid containing spt15, spread them evenly on a sterile plate, dry them with sterile wind, and use 20Kev energy to make 40×10 14 ions / cm 2 Nitrogen ion mutagenesis was carried out under the dose. After ion implantation, elution was performed with 1mLTE solution in a sterile environment. The eluted bacterial solution was incubated at 3...

Embodiment 2

[0049] This example illustrates the biological morphology and genetic stability of the mutant strain Saccharomyces cerevisiae SC-X234

[0050] Morphology and physiochemical characteristics of Saccharomyces cerevisiae SC-X234 of the present invention: the colony is milky white, facultative anaerobic, the colony size is 2-3mm, circular colony, the growth temperature is 35-40°C, the optimum pH is 5.0-5.5, Methylene blue staining: Live bacteria are colorless and dead bacteria are blue.

[0051] Carry out subculture experiment with the culture medium and culture condition among the embodiment 1, the result is shown in Table 2,

[0052] Table 2 Genetic stability of Saccharomyces cerevisiae SC-X234

[0053] Number of passages Alcohol production (v / v) 1 15.25 2 15 3 15 4 15.25 5 14.75 6 15.25

[0054] From the analysis of genetic stability test results, it can be known that the mutant strain Saccharomyces cerevisiae SC-X234 has stable al...

Embodiment 3

[0056] This example shows that Saccharomyces cerevisiae SC-X234 uses corn saccharification liquid to ferment alcohol

[0057] The culture medium formula described in the present embodiment is as follows:

[0058] Plate medium: glucose 2%, peptone 2%, agar 2%, yeast extract 2%, the rest is water, natural pH.

[0059] Incline medium: glucose 2%, peptone 2%, agar 2%, yeast extract 2%, the rest is water, natural pH.

[0060] Seed medium: 5% glucose, 0.5% yeast extract, 0.01% magnesium sulfate, 0.2% calcium chloride, 0.2% ammonium sulfate, 0.1% diammonium hydrogen phosphate, pH 5.5, and glucose needs to be dissolved.

[0061] Fermentation medium: 32% corn saccharification solution, 0.1% yeast extract, 0.01% calcium chloride, 1% ammonium sulfate, 0.5% diammonium hydrogen phosphate, 0.01% magnesium sulfate, pH5.5, in which glucose needs to be separated, corn saccharification solution It is the product of corn treated with liquefaction enzyme and glucoamylase.

[0062] Saccharomyce...

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Abstract

The invention discloses a strain of brew yeast and application of the brew yeast to manufacturing alcohol through fermentation. The brew yeast, (Saccharomyces cerevisiae) SC-X234, is collected on January 6th, 2014 at China Center for Type Culture Collection (CCTCC), with the collection number of CCTCC M2014005; the feeble train N<+> infusion mediate spt15 gene technology is adopted, SC-100 is used as initiation strain; primary screening is conducted by utilizing a TTC slab and an alcohol slab; the obtained strain is subjected to fermentation and secondary screening to gradually increase the tolerance of the yeast in alcohol; the manufacturing strain SC-X234 with the yield of 15.75% can be finally obtained; the strain can greatly increase the fermentation productivity; the sugar to alcohol conversion ratio is high; the alcohol yield in a 5 L fermentation tank can reach 126 g / L, which is 87.5% higher than that of the initiation strain; the alcohol has a very high economic value as a fuel alcohol.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a strain of Saccharomyces cerevisiae and its application in producing ethanol by fermentation. Background technique [0002] Fossil energy is the main body of today's energy structure, but it is a very valuable non-renewable resource with limited reserves. Taking my country as an example, although my country is a big oil producer, it is also a big oil consumer and a big shortage of oil resources. In 2008, my country produced 190 million tons of crude oil, consumed 365 million tons of crude oil, and imported 175 million tons of crude oil, accounting for 48% of total consumption. By the end of 2006, my country's accumulated proven recoverable oil reserves were 2.043 billion tons. According to this calculation, the existing oil can only last for decades, and the contradiction between oil supply and demand is very prominent and will continue to intensify. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/19C12P7/06C12R1/865
CPCY02E50/10
Inventor 虞龙陈晓双李玉燕刘媛
Owner NANJING TECH UNIV