Antibacterial modified chitosan foaming agent and preparation method thereof

[0092] Embodiment 1: prepare antibacterial modified chitosan foam agent

[0093] The implementation steps of this embodiment are as follows:

[0094] A, preparation of modified chitosan

[0095] To be sold by Jinan Haidebei Marine Bioengineering Co., Ltd. under the trade name of food and pharmaceutical grade chitosan, with a deacetylation degree of 70% and a molecular weight of 30×10 4 The chitosan of Da, dissolves 15min under the condition of stirring speed 250rpm in 2% acetic acid aqueous solution by weight, the solution that obtains is in the ultrasonic cleaning machine sold by trade name YD1008 ultrasonic cleaning machine by Changzhou Youda Ultrasonic Equipment Co., Ltd. In the process, ultrasonic treatment was carried out for 45 minutes under the conditions of ultrasonic frequency 20kHz and power 200W, and then the polyvinylidene fluoride ultrafiltration membrane sold by the American KENKER company under the trade name polyvinylidene fluoride membrane was used for classification, and in the hot air drying equipment Dry at a temperature of 60°C to obtain chitosan with a molecular weight of 900Da; add this chitosan to 50% potassium hydroxide aqueous solution by weight, treat it at a temperature of 116°C for 17min, and then use 2.0N hydrochloric acid The aqueous solution is neutralized, filtered, washed with distilled water, and dried at a temperature of 60° C. in the same drying equipment as above to obtain a modified chitosan with a deacetylation degree of 98% and a molecular weight of 500 Da;

[0096] B. White tea extraction

[0097] Weigh 15 parts by weight of white tea sold under the trade name Fuding White Tea by Fujian Pinpinxiang Tea Industry Co., Ltd., add 90 parts by weight of distilled water to it, then extract at a temperature of 95°C for 35 minutes, filter, and remove the tea dregs. The filtrate is fixed to 100 parts by weight to obtain the white tea extract with a mixture concentration of 0.016g/ml of tea polyphenols and caffeine, theophylline, tannic acid, etc.;

[0098] C, prepare the composite solution containing chitosan

[0099] 8 parts by weight of the modified chitosan prepared in step A are dissolved in the aqueous solution of glacial acetic acid prepared by 0.2 parts by weight of glacial acetic acid and 18 parts by weight of distilled water, then add 1.0 parts by weight of Tween 20, 0.6 parts by weight of Tween 80 , 0.3 parts by weight of sodium dodecyl alcohol polyoxyethylene ether sulfate sold under the trade name AES-2EO by Shanghai Xianjie Chemical Technology Co., Ltd. and 1.5 parts by weight of dodecane sold under the trade name K12 by Shanghai Youyang Industrial Co., Ltd. After dissolving, add 2.1 parts by weight of the white tea extract prepared in step B, 0.15 parts by weight of allyltriphenylphosphine bromide prepared according to the patented technology of Jinan University CN102020675B and 0.38 parts by weight of Shaanxi Senfu Natural Products Co., Ltd. sells ascorbyl palmitate under the trade name L-ascorbyl palmitate, continue to stir, and obtain a complex solution containing chitosan after dissolving;

[0100] D. Preparation of corn protein-containing compound solution

[0101] 0.1 parts by weight of corn protein sold by Shandong Litong Biotechnology Co., Ltd. under the trade name corn gluten powder, 0.5 parts by weight of agarose sold by Fujian Mingfu Agar Co., Ltd. under the trade name agar powder, 0.2 parts by weight of Shandong Shouguang Huatian Sugar Alcohol Co., Ltd. sells sorbitol under the trade name solid sorbitol, and 0.06 parts by weight of chondroitin sulfate sold under the trade name chondroitin sulfate by Anhui Kebao Bioengineering Co., Ltd. are dissolved in 42 parts by weight of distilled water to obtain a corn-containing Protein complex solution;

[0102] E, preparation of antibacterial modified chitosan foam

[0103] Take by weighing 4 parts by weight of glycerol, add water, stir evenly, then add 32 parts by weight of the composite solution containing chitosan obtained in step C and 40 parts by weight of the composite solution containing corn protein obtained in step D, stir well , to obtain the antibacterial modified chitosan foam.

[0104] According to the detection and analysis of the national standard antibacterial detection method described in this manual, the antibacterial modified chitosan foam prepared by the present embodiment has an antibacterial ability of 99.4% to Staphylococcus aureus, Escherichia coli, Streptococcus, and Pseudomonas aeruginosa .

[0105] Embodiment 2: prepare antibacterial modified chitosan foam agent

[0106] The implementation steps of this embodiment are as follows:

[0107] A, preparation of modified chitosan

[0108] To be sold by Jinan Haidebei Marine Bioengineering Co., Ltd. under the trade name of food and pharmaceutical grade chitosan, with a deacetylation degree of 70% and a molecular weight of 40×10 4 The chitosan of Da, dissolves 8min under the condition of stirring speed 280rpm in 1% acetic acid aqueous solution by weight, the solution that obtains is in the ultrasonic cleaning machine sold by trade name YD1008 ultrasonic cleaning machine by Changzhou Youda Ultrasonic Equipment Co., Ltd. In the process, ultrasonic treatment was carried out for 30 minutes under the conditions of ultrasonic frequency 22kHz and power 180W, and then the polyvinylidene fluoride ultrafiltration membrane sold by the American KENKER company under the trade name polyvinylidene fluoride membrane was used for classification, and in the hot air drying equipment Dry at a temperature of 60°C to obtain chitosan with a molecular weight of 500Da; add this chitosan to 40% potassium hydroxide aqueous solution by weight, treat it at a temperature of 110°C for 20min, and then use 1.0N hydrochloric acid The aqueous solution is neutralized, filtered, washed with distilled water, and dried at a temperature of 60° C. in the same drying equipment as above to obtain a modified chitosan with a deacetylation degree of 99% and a molecular weight of 650 Da;

[0109] B. White tea extraction

[0110] Weigh 18 parts by weight of white tea sold under the trade name Fuding White Tea by Fujian Pinpinxiang Tea Industry Co., Ltd., add 110 parts by weight of distilled water to it, then extract at a temperature of 100°C for 25 minutes, filter, remove tea dregs, and obtain the The filtrate is settled to 100 parts by weight, and the white tea extract that obtains the mixture concentration such as tea polyphenols and caffeine, theophylline, tannic acid is 0.019g/ml;

[0111] C, prepare the composite solution containing chitosan

[0112] 2 parts by weight of the modified chitosan prepared in step A are dissolved in the aqueous solution of glacial acetic acid prepared by 0.1 parts by weight of glacial acetic acid and 20 parts by weight of distilled water, then add 0.2 parts by weight of Tween 20, 0.2 parts by weight of Tween 80 , 0.4 parts by weight of sodium lauryl alcohol polyoxyethylene ether sulfate sold under the trade name AES-2EO by Shanghai Xianjie Chemical Technology Co., Ltd. and 1.2 parts by weight of dodecane sold under the trade name K12 by Shanghai Youyang Industrial Co., Ltd. After dissolving, add 1.5 parts by weight of the white tea extract prepared in step B, 0.05 parts by weight of allyltriphenylphosphine bromide prepared according to the patented technology of Jinan University CN102020675B and 0.30 parts by weight of Shaanxi Senfu Natural Products Co., Ltd. sells ascorbyl palmitate under the trade name L-ascorbyl palmitate, continue to stir, and obtain a complex solution containing chitosan after dissolving;

[0113] D. Preparation of corn protein-containing compound solution

[0114] 0.4 parts by weight of corn protein sold by Shandong Litong Biotechnology Co., Ltd. under the trade name corn gluten powder, 0.2 parts by weight of agarose sold by Fujian Mingfu Agar Co., Ltd. under the trade name agar powder, 0.2 parts by weight of Shandong Shouguang Huatian Sugar Alcohol Co., Ltd. sells sorbitol under the trade name solid sorbitol, and 0.01 parts by weight of chondroitin sulfate sold under the trade name chondroitin sulfate by Anhui Kebao Bioengineering Co., Ltd. are dissolved in 18 parts by weight of distilled water to obtain a corn-containing Protein complex solution;

[0115] E, preparation of antibacterial modified chitosan foam

[0116] Take by weighing 8 parts by weight of glycerol, add water, stir evenly, then add 25 parts by weight of the composite solution containing chitosan obtained in step C and 30 parts by weight of the composite solution containing corn protein obtained in step D, stir well , to obtain the antibacterial modified chitosan foam.

[0117]According to the detection and analysis of the national standard antibacterial detection method described in this manual, the antibacterial modified chitosan foam prepared by this embodiment has an antibacterial ability of 99.3% to Staphylococcus aureus, Escherichia coli, Streptococcus, and Pseudomonas aeruginosa. .

[0118] Embodiment 3: prepare antibacterial modified chitosan foam agent

[0119] The implementation steps of this embodiment are as follows:

[0120] A, preparation of modified chitosan

[0121] To be sold by Jinan Haidebei Marine Bioengineering Co., Ltd. under the trade name of food and pharmaceutical grade chitosan, deacetylation degree 70%, molecular weight 50×10 4 The chitosan of Da, dissolves 12min under the condition of stirring speed 320rpm in 1% acetic acid aqueous solution by weight, the solution that obtains is in the ultrasonic cleaning machine sold by trade name YD1008 ultrasonic cleaning machine by Changzhou Youda Ultrasonic Equipment Co., Ltd. In the process, ultrasonic treatment was carried out for 50 minutes under the conditions of ultrasonic frequency 24kHz and power 220W, and then the polyvinylidene fluoride ultrafiltration membrane sold by the American KENKER company under the trade name polyvinylidene fluoride membrane was used for classification, and in the hot air drying equipment Dry at a temperature of 60°C to obtain chitosan with a molecular weight of 800Da; add this chitosan to 42% potassium hydroxide aqueous solution by weight, treat it at a temperature of 114°C for 19min, and then use 2.5N hydrochloric acid The aqueous solution is neutralized, filtered, washed with distilled water, and dried at a temperature of 60° C. in the same drying equipment as above to obtain a modified chitosan with a deacetylation degree of 98% and a molecular weight of 820 Da;

[0122] B. White tea extraction

[0123] Weigh 22 parts by weight of white tea sold under the trade name Fuding White Tea by Fujian Pinpinxiang Tea Industry Co., Ltd., add 80 parts by weight of distilled water to it, then extract at a temperature of 98°C for 32 minutes, filter, remove tea residue, and obtain the The filtrate is settled to 100 parts by weight, and the white tea extract that obtains the mixture concentration such as tea polyphenols and caffeine, theophylline, tannic acid is 0.024g/ml;

[0124] C, prepare the composite solution containing chitosan

[0125] 6 parts by weight of the modified chitosan prepared in step A are dissolved in the aqueous solution of glacial acetic acid prepared by 0.2 parts by weight of glacial acetic acid and 22 parts by weight of distilled water, then add 0.6 parts by weight of Tween 20, 1.0 parts by weight of Tween 80 , 0.6 parts by weight of sodium dodecyl alcohol polyoxyethylene ether sulfate sold under the trade name AES-2EO by Shanghai Xianjie Chemical Technology Co., Ltd. and 0.9 parts by weight of dodecane sold under the trade name K12 by Shanghai Youyang Industrial Co., Ltd. Sodium sulfate, after dissolving, add 1.8 parts by weight of the white tea extract prepared in step B, 0.07 parts by weight of allyltriphenylphosphine bromide prepared according to the patented technology of Jinan University CN102020675B and 0.44 parts by weight of Shaanxi Senfu Natural Products Co., Ltd. sells ascorbyl palmitate under the trade name L-ascorbyl palmitate, continue to stir, and obtain a complex solution containing chitosan after dissolving;

[0126] D. Preparation of corn protein-containing compound solution

[0127] 0.8 parts by weight of corn protein sold by Shandong Litong Biotechnology Co., Ltd. under the trade name corn gluten powder, 0.9 parts by weight of agarose sold by Fujian Mingfu Agar Co., Ltd. under the trade name agar powder, 0.1 parts by weight of Shandong Shouguang Huatian Sugar Alcohol Co., Ltd. sells sorbitol under the trade name solid sorbitol, and 0.03 parts by weight of chondroitin sulfate sold under the trade name chondroitin sulfate by Anhui Kebao Bioengineering Co., Ltd. are dissolved in 28 parts by weight of distilled water to obtain a corn-containing Protein complex solution;

[0128] E, preparation of antibacterial modified chitosan foam

[0129] Take by weighing 5 parts by weight of glycerol, add water, stir evenly, then add 28 parts by weight of the composite solution containing chitosan obtained in step C and 32 parts by weight of the composite solution containing corn protein obtained in step D, stir well , to obtain the antibacterial modified chitosan foam.

[0130] According to the detection and analysis of the national standard antibacterial detection method described in this manual, the antibacterial modified chitosan foam prepared by the present embodiment has an antibacterial ability of 99.8% to Staphylococcus aureus, Escherichia coli, Streptococcus, and Pseudomonas aeruginosa .