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A compound probiotic peptide and its preparation method and application

The technology of probiotics and probiotics is applied in the field of compound probiotic peptides and its preparation, which can solve the problems of hindering the digestion, absorption and utilization of nutrients by animals, endangering animal health and growth and development, etc., and achieves good industrialization implementation prospects and costs. Inexpensive, palatability-enhancing effect

Active Publication Date: 2016-08-31
九源岳阳生物科技饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the presence of anti-nutritional substances such as protease inhibitors, lectins, soybean antigenic proteins, urease, phytic acid, soybean oligosaccharides in soybeans not only greatly hinders the digestion, absorption and utilization of nutrients by animals, but also seriously Harmful to the health and growth of animals

Method used

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  • A compound probiotic peptide and its preparation method and application
  • A compound probiotic peptide and its preparation method and application
  • A compound probiotic peptide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Isolation and screening of Bacillus pumilus S3

[0034] From May 2013 to September 2013, water samples, sediment, and intestinal feces of prawns were collected from many places along the coast of Jiaozhou. The bottom mud and shrimp intestinal feces samples were diluted 10 times in sterile normal saline, fully ground, and the supernatant was taken as the sample solution; the water sample was directly used as the sample solution; the sample solution was inoculated in 2216E liquid medium for enrichment at 30°C After culturing for 48 hours, it was diluted and spread on a 2216E plate to isolate and purify the strains. A total of 26 pure strains were screened out.

[0035] The purified single strains were screened for antagonistic bacteria by plate spotting method. Using Vibrio parahaemolyticus as indicator bacteria, Vibrio parahaemolyticus was inoculated in 2216E liquid medium and cultured for 18 hours, and diluted to 10 5 -10 6 For the CFU / mL bacterial suspension, tak...

Embodiment 2

[0057] The contents of the four kinds of bacteria in the wet material at the end of fermentation, the compound probiotic peptide product after low-temperature drying, and the compound probiotic peptide product stored at room temperature for 6 months were measured respectively. Use tryptone-sulfite-cycloserine medium to measure the amount of Clostridium butyricum by sandwich culture method; use nutrient agar medium to measure the amount of Bacillus pumilus S3 by dilution coating method; use MRS medium , using the pouring culture method to determine the amount of Lactobacillus plantarum; using YPD medium, using the dilution coating method to determine the amount of Saccharomyces boulardii. In view of the large difference in the amount of the four types of bacteria, the amount of bacteria was expressed in log CFU / g.

[0058] figure 1 The composition and half-year stability of the complex prebiotic peptide-probiotic bacteria prepared by No. 1 soybean meal after biological treatme...

Embodiment 3

[0060] SDS-PAGE (polyacrylamide gel electrophoresis) was used to qualitatively determine the degradation of the antigenic protein, and an ELISA (enzyme-linked immunosorbent assay) kit was used to quantitatively determine the removal rate of the antigenic protein. Use TCA (trichloroacetic acid extraction method) to determine acid-soluble protein (accounting for crude protein, %); refer to GB / T 19541-2004 soybean meal for feed, use 0.2% KOH to determine the solubility of KOH, refer to the national standard GBT-8314-2002 free Determination of the total amount of amino acids and the content of free amino acids; using the SDS-Ⅱ monogastric animal bionic digestive system developed by the Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, to simulate the determination of in vitro protein digestibility.

[0061] The SDS-PAGE qualitative determination result of antigenic protein is as follows image 3 As shown, the ELISA quantitativ...

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Abstract

The invention discloses a compound probiotic peptide and its preparation method and application. It is fermented by adding a bacterial enzyme compound starter to soybean meal. The bacterial enzyme compound starter consists of four kinds of probiotics and four kinds of proteases. The four probiotics are Clostridium butyricum, Bacillus pumilus S3, Lactobacillus plantarum and Saccharomyces boulardii; the four proteases are acid protease, neutral protease, alkaline protease and polypeptide protease. It also provides a specific preparation method of the compound probiotic peptide and its application in the preparation of feed additives for suckling piglets. The compound probiotic peptide product is rich in a variety of probiotics, which can effectively degrade antigenic proteins to produce small molecular substances such as polypeptides and amino acids, and produce a variety of probiotic organic acids, biotin and other flavor and antagonistic substances during the fermentation process. It not only increases the palatability and safety, but also significantly improves the utilization rate of soybean meal and the nutritional value of feed, and can also significantly reduce the diarrhea rate of suckling piglets.

Description

technical field [0001] The invention belongs to the field of biological enzymolysis and fermentation, and relates to a preparation method of peptides, in particular to a compound probiotic peptide and its preparation method and application. Background technique [0002] As an important plant protein source, soybean meal has important application and research value. However, the presence of anti-nutritional substances such as protease inhibitors, lectins, soybean antigenic proteins, urease, phytic acid, and soybean oligosaccharides in soybeans not only greatly hinders the digestion, absorption and utilization of nutrients by animals, but also seriously Harmful to the health and growth of animals. Soybean antigenic protein is a soybean antinutritional factor with the highest residual amount and the strongest antinutritional effect in soybean processed products. It can cause allergic reactions in the intestinal tract and other organs of infants, mice, piglets, calves and other...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/00A23K10/16
Inventor 李慧芬张大伟马成
Owner 九源岳阳生物科技饲料有限公司
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