Method for reducing fluorine content in water soluble active peptides from Antarctic krill
An Antarctic krill and active peptide technology, applied in fermentation and other directions, can solve the problems of complex equipment, low solubility, high cost, etc., and achieve the effects of less investment in equipment, high product safety, and high product recovery rate
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[0021] Example 1
[0022] 1) Take 5kg of Antarctic krill powder, add 60L of distilled water to the krill powder, add 2M HCl to the mixture, adjust the pH of the mixture to 7.5, heat up to 45°C, add 75g of neutral protease to the mixture, Stir in the reaction kettle at constant temperature for 10 hours, heat to 100°C to terminate the enzyme reaction, centrifuge to obtain supernatant A;
[0023] 2) Through vacuum concentration, the solid content of the supernatant A reaches 15%, the vacuum degree is 0.08 MPa, and the temperature is 40 to 80°C to obtain solution B;
[0024] 3) Take 10L of solution B, add 100g of calcium hydroxide to it, mix well, and cool to obtain mixed solution C;
[0025] 4) Add 184g of calcium monohydrogen phosphate (calcium hydrogen phosphate) to mixed solution C, and stir while adding to obtain mixed solution D;
[0026] 5) After the mixture D is centrifuged at 5000r / min, the supernatant E is obtained;
[0027] 6) The supernatant E is spray-dried (inlet air temperatu...
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[0032] Example 2
[0033] 1) Take 10 kg of Antarctic krill powder, add 100L of 95% ethanol to the krill powder, stir in the reactor for 6 hours, centrifuge to collect the precipitate, and vacuum dry to obtain defatted krill powder;
[0034] 2) Take 5kg of defatted shrimp powder, add 50L of distilled water to the defatted shrimp powder, add 2M HCl to the mixture, adjust the pH of the mixture to 8.0, heat up to 50℃, add 80g of alkaline protease to the mixture, and hydrolyze at constant temperature 8h, heat to 100℃ to terminate the enzyme reaction, centrifuge to get supernatant A;
[0035] 3) Through vacuum concentration, the solid content of the supernatant A reaches 10%, the vacuum degree is 0.08 MPa, and the temperature is 40 to 80°C to obtain solution B;
[0036] 4) Take 10L of solution B, add 80g of calcium oxide to it, mix well, and cool to obtain mixed solution C;
[0037] 5) Add 334g of calcium dihydrogen phosphate to mixed liquid C, and stir while adding to obtain mixed liquid D;...
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[0041] Example 3
[0042] 1) Take 10 kg of Antarctic krill powder, add 100L of 95% ethanol to the krill powder, stir in the reactor for 6 hours, centrifuge to collect the precipitate, and vacuum dry to obtain defatted krill powder;
[0043] 2) Take 5kg of defatted shrimp powder, add 50L of distilled water to the defatted shrimp powder, heat up to 50℃, add 2M HCl to the mixture to adjust the pH of the mixture to 8.0, add 80g of alkaline protease to the mixture, and hydrolyze at constant temperature for 8h , Heat to 100°C to terminate the enzyme reaction, centrifuge to get supernatant A;
[0044] 3) Through vacuum concentration, the solid content of the supernatant A reaches 10%, the vacuum degree is 0.08 MPa, and the temperature is 40 to 80°C to obtain solution B;
[0045] 4) Take 10L of solution B, add 150g of calcium chloride to it, mix well, and cool to obtain mixed solution C;
[0046] 5) After the mixture C is centrifuged at 4500r / min, the supernatant D is obtained;
[0047] 7) The ...
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