Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing peptide-enriched product with high content of glutamine-bound peptide

A high-glutamine, peptide-binding technology, applied in the fields of medicine, food, and agricultural product processing, can solve the problems of high enzyme consumption, substrate waste, and product separation, and achieve the effect of reducing free amino acid content and substrate inhibition

Active Publication Date: 2015-04-22
苏州朗邦营养科技有限公司
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current process not only consumes a lot of energy, but also generally has the disadvantages of batch operation in the production process, high enzyme consumption, low protein conversion rate, and the product cannot be separated from the reaction system in time, resulting in waste of substrates and high process costs.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing peptide-enriched product with high content of glutamine-bound peptide
  • Method for preparing peptide-enriched product with high content of glutamine-bound peptide
  • Method for preparing peptide-enriched product with high content of glutamine-bound peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Water and wheat protein (gu Nguyen powder) are added to the mixing tank for slurry mixing, the temperature is 60° C., the pH is 10, the mass concentration of wheat protein is 50%, and the alkaline protease ( 40U / g wheat protein) and papain (40U / g wheat protein); after 3 hours of reaction, the wheat protein slurry was added to the enzyme reactor, the temperature was set at 80°C, the pH was 10, and the total activity of 80U / g wheat was added trypsin, trypsin, alkaline protease, neutral protease and compound flavor protease for enzymolysis of protein, and at the same time, the enzymolysis solution is continuously introduced into the vibrating membrane (ultrafiltration with a molecular cut-off of 10kDa) equipment from the bottom of the enzyme reactor, The small molecule target peptide is filtered out in time through the vibrating membrane device, and the macromolecular substrate and enzyme are returned to the enzyme reactor to continue the enzymatic hydrolysis reaction, impr...

Embodiment 2

[0033]Add water and wheat protein (guruan powder) to the mixing tank for mixing, the temperature is 40°C, the pH is 4, the concentration of wheat protein is 50%, add 20U / g acid protease, and the wheat protein slurry is reacted for 1 hour Add it into the enzymolysis tank, set the temperature to 40°C and pH to 4, add 20U / g of acid protease, neutral protease and compound flavor protease for enzymolysis, and continuously introduce the vibrating membrane from the bottom of the tank (the molecular cut-off flow is 10kDa In the ultrafiltration) equipment, the small molecular target peptide is filtered out in time through the vibrating membrane equipment, and the macromolecular substrate and enzyme are returned to the enzyme reaction tank to continue the enzymatic hydrolysis reaction, improving the conversion rate of protein and the utilization rate of enzyme. The operating pressure of the vibrating membrane ultrafiltration membrane is 0.02Mpa, and the circulation flow rate is 20L / h. W...

Embodiment 3

[0035] Add water and wheat protein (gu Nguyen powder) to the mixing tank for mixing, the temperature is 50°C, the pH is 8, the concentration of wheat protein is 45%, add 50U / g of neutral protease and alkaline protease, and react 1 Hours later, the wheat protein slurry was added to the enzymolysis tank, the temperature was set at 60°C, the pH was 6, 40U / g of acid protease, neutral protease and compound flavor protease were added for enzymolysis, and the vibrating membrane was continuously introduced from the bottom of the enzyme reactor (Ultrafiltration with a molecular cutoff of 8kDa) equipment, the small molecule target peptide is filtered out in time through the vibrating membrane device, and the macromolecular substrate and enzyme are returned to the enzyme reaction tank to continue the enzymatic hydrolysis reaction, improving the conversion rate of protein and the activity of enzyme. utilization rate. The operating pressure of the vibrating membrane ultrafiltration membran...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing a peptide-enriched product with high content of glutamine-bound peptide, and belongs to the technical field of processing of medicines, foods, agricultural products. According to the method, wheat protein serving as a raw material is firstly sized and mixed, and then is subjected to compound protease enzymolysis in an enzyme membrane reactor which is formed by coupling vibration membrane equipment and an enzyme reactor, so that continuous separation of small-molecular-weight hydrolysate can be realized, macro-molecular substrate and enzyme can be trapped, substrate inhibiting effect can be reduced, the content of free amino acid can be reduced, and the defect of a traditional intermittent enzymolysis method can be overcome. The prepared peptide-enriched product with high content of glutamine-bound peptide has water solubility of more than 99 percent, polypeptide content of more than 90 percent, content of glutamine-bound peptide of more than 30 percent and ash content of less than 1 percent.

Description

technical field [0001] The invention relates to a method for preparing a peptide-rich product with high glutamine-binding peptide content, in particular to a method for preparing a peptide-rich product with high glutamine-binding peptide content from wheat protein, and belongs to the technical fields of medicine, food and agricultural product processing. Background technique [0002] Wheat hydrolyzed protein is a functional peptide-rich product that is made from wheat protein (gluten) and enzymatically hydrolyzed. It is a protein material with high nutritional value, good digestibility and health care function. Wheat hydrolyzed protein has been widely used in food fields such as health care products, meat products, bakery products, etc., and can replace expensive plasma proteins with high risk of pathogenicity in feed as calf milk replacer, suckling pig creep feed, salmon and other high-grade aquatic feed , Wheat hydrolyzed protein is an excellent protein that does not conta...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C12M1/38C12M1/36C12M1/12C12M1/02C12M1/00
Inventor 赵伟
Owner 苏州朗邦营养科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com