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A kind of efficient synthesis and extraction method of blueberry anthocyanin

A blueberry anthocyanin and extraction method technology, which is applied in the field of efficient synthesis and extraction of blueberry anthocyanin, can solve the problems of difficult dissolution of target components, large loss of active components, high cost, etc., to ensure stability, improve the purity of finished products, and reduce utilization easy effect

Active Publication Date: 2018-07-17
广东维思奇食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the defects that blueberry anthocyanins are mainly concentrated in blueberry cells, and a large amount of anthocyanin precursors are not fully synthesized and utilized, resulting in difficult dissolution of target components, large loss of active components, high energy consumption and high cost, and propose A method for cultivating blueberry cells to synthesize anthocyanins, establishing a synthesis and extraction method of blueberry anthocyanins with high extraction rate, strong operability, low cost and mild conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Raw material pretreatment: take fresh, non-rotten blueberry fruit as raw material, wash and mechanically mash it until it is pulpy for later use;

[0025] (2) Blueberry cell culture: the blueberry homogenate in step (1) was subjected to 0.7 mmol·L -1 Treat with Triton X-100 for 15-20 minutes, high hydrostatic pressure (HHP) of 55-58Mpa for 3-4 minutes, inoculate: Aspergillus niger 6%, Monascus 6%, Bacillus licheniformis 1%, Rhodotorula viscosus 5 %, add L-phenylalanine 30mg / L, L-tyrosine 5mg / L, add the mass ratio of L-phenylalanine and L-tyrosine to 1:1, medium formula: glucose 0.4%, corn steep liquor 1.5%, NaCl 0.4%, K 2 HPO 4 0 . 2%, pH 2.5, cultivated at 28°C for 7 days, and collected the culture solution;

[0026] (3) Separation and concentration of the extraction mixture: centrifuge the fermentation broth of step (2) at 80,000 r / min for 10-20 min at room temperature, discard the precipitate, and collect the supernatant; ultrafilter the supernatant at a pre...

Embodiment 2

[0030] (1) Raw material pretreatment: take fresh, non-rotten blueberry fruit as raw material, wash and mechanically mash it until it is pulpy for later use;

[0031] (2) Blueberry cell culture: the blueberry homogenate in step (1) was subjected to 0.7 mmol·L -1 Treat with Triton X-100 for 15-20 minutes, and treat with high hydrostatic pressure (HHP) of 55-58Mpa for 3-4 minutes, inoculate: Monascus 6%, lactic acid bacteria 0.6%, Rhodotorula viscosus 5%, Saccharomyces cerevisiae 1.2%, Add L-phenylalanine 30 mg / L, L-tyrosine 6 mg / L, add the mass ratio of L-phenylalanine and L-tyrosine to 1:1, medium formula: sucrose 0.5%, corn steep liquor 2.0%, NaCl 0.5%, K 2 HPO 4 0.3%, pH 3.5, cultivated at 28°C for 7 days, and collected the culture medium;

[0032] (3) Separation and concentration of the extraction mixture: centrifuge the fermented liquid of step (2) at room temperature at 9000r / min for 15min, discard the precipitate, and collect the supernatant; ultrafilter the supernata...

Embodiment 3

[0036] (1) Raw material pretreatment: take fresh, non-rotten blueberry fruit as raw material, wash and mechanically mash it until it is pulpy for later use;

[0037] (2) Blueberry cell culture: the blueberry homogenate in step (1) was subjected to 0.7 mmol·L -1 Triton X-100 treatment for 10-15 minutes, 55-58Mpa high hydrostatic pressure (HHP) treatment for 3-4 minutes, inoculation: Monascus 7%, lactic acid bacteria 0.8%, Rhodotorula viscosus 6%, Saccharomyces cerevisiae .5% , the mass ratio of adding L-phenylalanine to L-tyrosine is 1:1, the medium formula: 0.5% sucrose, 11.8% peptone, the mass ratio of glucose and corn steep liquor is 1:2, NaCl 0.6%, K 2 HPO 4 0.3%, pH 3.0, cultivated at 28~30°C for 7 days, and collected the culture medium;

[0038] (3) Separation and concentration of the extraction mixture: centrifuge the fermentation broth of step (2) at 10,000 r / min at room temperature for 15 min, discard the precipitate, and collect the supernatant; ultrafilter the su...

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Abstract

The invention discloses a method for efficiently synthesising and extracting anthocyanin in blueberry, and specifically discloses a synthesis and extraction preparation technology for blueberry anthocyanin. The method comprises the following steps: culturing blueberry cells by high hydrostatic-pressure assisted microbial fermentation, and extracting blueberry anthocyanin by ultrafiltration chromatography; breaking the cell walls of blueberry fruits by virtue of a high hydrostatic-pressure assisted microbial metabolite to adequately release anthocyanin; adding producing enzymes of L-phenylalanine, rhodotorula glutinis and the like to promote synthesis for precursors which are not converted to anthocyanin in blueberry into anthocyanin simultaneously; subjecting the fermentation liquor to treatment of centrifuging, ultrafiltration separation, chromatographic purification, vacuum freeze-drying and the like to obtain anthocyanin with the purity of 99.31%, wherein the extraction amount of anthocyanin is increased by 15-30%. The method disclosed by the invention is simple to operate, clean and harmless in generation process, low in cost, high in anthocyanin yield, high in purity, without the need of human intestinal microbial degradation, high in medical treatment health value, suitable for large-scale production application, and extremely high in market prospect.

Description

technical field [0001] The invention relates to a method for efficiently synthesizing and extracting blueberry anthocyanins, which belongs to the technical field of bioengineering. Background technique [0002] Numerous studies have shown that dietary regulation and nutritional supplementation can improve human health and prevent disease. Reasonable nutritional therapy can improve the therapeutic effect of traditional medicine, reduce the amount of medicine used, and thus reduce the side effects of drug treatment. In recent years, studies have found that blueberries are rich in a large amount of natural anthocyanins, which have antioxidant capacity, can improve human body functions and prevent certain eye diseases. Therefore, how to efficiently extract and utilize anthocyanins in blueberries has become an emerging frontier research topic in the 21st century. [0003] The anthocyanins obtained by the separation and extraction technology currently used: low content, many imp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P17/06C12R1/685C12R1/66C12R1/10C12R1/865
Inventor 叶澄
Owner 广东维思奇食品有限公司
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