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Bacillus subtilis and biological deodorant containing bacterial strain of bacillus subtilis

A technology of Bacillus subtilis and biological deodorant, applied in the field of Bacillus subtilis and biological deodorant containing the strain, can solve the problems of water odor, affecting the survival of aquatic organisms, etc., to reduce the production of malodorous gas, significantly eliminate the Odor effect, remarkable effect

Active Publication Date: 2015-05-13
QINGDAO WEILAN TIANCHENG BIOLOGICAL TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Malodorous substances are generally distributed in the atmosphere, and some will enter the water body with waste water and waste residue, which not only makes the water smelly, but also affects the survival of aquatic organisms

Method used

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  • Bacillus subtilis and biological deodorant containing bacterial strain of bacillus subtilis
  • Bacillus subtilis and biological deodorant containing bacterial strain of bacillus subtilis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Separation, screening and identification of ammonia-reducing and deodorizing functional microorganisms

[0017] 1. Culture medium:

[0018] Nutrient agar medium;

[0019] Selective media:

[0020] name Weight (g) NH 4 Cl 0.125 NaCl 0.25 K H 2 PO 4 1.6 K 2 HPO 4 0.5 MnCl 2 2H 2 o 0.05 MgSO 4 ·7H 2 o 0.25 MoCl 2 0.002 glucose 2.50 Bromothymol blue (1% ethanol solution) 1.0 agar 20.0 pure water 1000

[0021] 2. Screening method

[0022] (1) Sample: selected from chicken manure and surrounding soil in the manure storage tank of Pingdu chicken farm in Qingdao;

[0023] (2) Separation and purification method: using the dilution plate method;

[0024] Take 10g samples of chicken manure and surrounding soil in the manure storage tank of the chicken farm, mix well, add 100mL sterile normal saline and mix well. Take 1mL of the suspension and add it to 9mL of sterile norma...

Embodiment 2

[0035] Example 2 The application effect of Bacillus subtilis ZNX3 in chicken house deodorization

[0036] Activate Bacillus subtilis ZNX3, expand the culture, collect the sludge through liquid fermentation and centrifugation, and make a bacterial suspension to make the bacterial count reach 10 6 cfu / mL. Spray the bacterial suspension evenly in the chicken house, and try to make the spraying range cover the whole chicken house.

[0037] In different directions of the chicken house, at a height of about 1.5m from the ground, select 7 different sampling points, and use ammonia and hydrogen sulfide detection instruments to measure ammonia and hydrogen sulfide in the chicken house before spraying and 10 minutes after spraying concentration, and calculate the average concentration of ammonia and hydrogen sulfide, the specific results are shown in Table 2.

[0038] Table 2 Concentration comparison of ammonia and hydrogen sulfide in the chicken house before and after spraying Bacill...

Embodiment 3

[0042] Example 3 The deodorizing effect of the biological deodorizer comprising Bacillus subtilis ZNX3 on chicken manure

[0043] 1. Deodorant sample preparation

[0044] Activate Bacillus subtilis ZNX3, expand the culture, collect the sludge through liquid fermentation and centrifugation, and take the equal quality of the sludge for the following treatment:

[0045] (1) Sample 1: Resuspend Bacillus subtilis ZNX3 bacteria sludge with physiological saline to make a bacterial suspension, the amount of viable bacteria is about 10 7 cfu / mL;

[0046] (2) Sample 2: Mix Bacillus subtilis ZNX3 sludge with yeast and / or lactic acid bacteria to make a mixed bacterial suspension, the total amount of viable bacteria is about 10 7 cfu / mL, of which the proportion of Bacillus subtilis ZNX3 is not less than 70%;

[0047] (3) Sample 3: mix Bacillus subtilis ZNX3 bacterium slime with 5% NBPT (n-butylthiophosphate triamine) propylene glycol aqueous solution with a mass volume ratio of 5%, to mak...

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Abstract

The invention relates to the technical field of function microbiological screening, and particularly provides bacillus subtilis ZNX3 with a deodorizing function. The preservation number of bacillus subtilis ZNX3 is CCTCC NO: M2014649; on the one hand, the bacterial strain can effectively reduce the amount of release of foul gases such as ammonia gas and hydrogen sulfide in manure of livestock and poultry, reduces the generation of the foul gases based on the source, and achieves a remarkable effect as the ammonia gas concentration released by the chicken manure on the fifth day is only 6.7% of a control group; on the other hand, the bacterial strain can quickly degrade ammonia gas and hydrogen sulfide in a henhouse, 10 minutes after spraying, the ammonia gas concentration in the henhouse is reduced by 95.7%, and the hydrogen sulfide concentration is reduced by 94.7%. In addition, the bacillus subtilis ZNX3 can effectively reduce the amount of release of foul gases in household garbage and sewage, the removal rates of ammonia gas, hydrogen sulfide and total hydrocarbon are all higher than 95%, and an unexpected technical effect is achieved.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a bacillus subtilis and a biological deodorant containing the strain. Background technique [0002] With the development of large-scale and intensive animal husbandry, the pollution of the environment by the odor produced in the production of livestock and poultry is increasing. As one of environmental hazards, stench can stimulate people's olfactory nerve and trigeminal nerve, and poison the respiratory center, which not only poses a great threat to human health, causes air pollution, but also seriously affects the health of livestock (poultry) And production performance, so the odor pollution of livestock farms has attracted more and more attention. Taking ammonia as an example, if the volume fraction of ammonia in the air in the living environment of young pigs reaches 0.00005, the weight gain rate of young pigs will drop by 12%; if it reaches 0.000...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F11/02C02F3/34B09B3/00A61L9/01C12R1/125C12R1/07A61L101/52
CPCA61L9/01A61L2209/21B09B3/00C02F3/34C02F3/347C02F11/02C12N1/16C12N1/20C12N1/205C12R2001/125
Inventor 郑玉莲苟万里甄丽朱彩彩
Owner QINGDAO WEILAN TIANCHENG BIOLOGICAL TECH
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