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A kind of candida tropicalis dk2 bacterial strain and application thereof

A technology of Candida tropicalis and strains, which is applied to fungi, bacterial retting, microorganisms, etc., can solve the problems of high energy consumption of chemical degumming process, unrecyclable waste water, and large equipment loss, etc., and achieves high degumming efficiency and degumming. Short cycle time, good quality effect

Inactive Publication Date: 2018-10-02
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the scouring methods commonly used in the hemp industry are mainly chemical methods and enzymatic degumming methods. The chemical degumming process consumes a lot of energy and equipment loss, and the discharged wastewater cannot be recycled, causing serious pollution problems.

Method used

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  • A kind of candida tropicalis dk2 bacterial strain and application thereof
  • A kind of candida tropicalis dk2 bacterial strain and application thereof
  • A kind of candida tropicalis dk2 bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Screening method for retting or scouring strains of hemp fiber and bamboo fiber:

[0040] (1) Decomposed hemp and its soil were obtained from the retting base, and the decomposed hemp and its soil and the PDA medium were placed in the PDA medium at a ratio of 1 g: 20 ml, and enriched for 10 days. Then take 0.1ml of the PDA culture and apply it to the separation medium, and culture it statically at 30°C for 1-4 days to obtain a wild-type biologically treated high-quality strain.

[0041] (2) Inoculate the bacterial strain obtained in step (1) into PDA medium, and culture at 30° C. for 72 hours. Add freezing buffer and store at -80°C.

[0042] Described step (1) PDA medium formula is: potato 200g, glucose 20g, agar 15g, distilled water 1000mL, pH is natural.

[0043] The formula of step (2) per 100ml of freezing buffer: potassium dihydrogen phosphate 0.1g, dipotassium hydrogen phosphate 0.02g, sodium citrate 0.06g, magnesium sulfate heptahydrate 0.03g, glycerol 12ml, ad...

Embodiment 2

[0046] Utilize the method for preparing laccase from Candida tropicalis DK2 strain:

[0047] (1) Candida tropicalis DK2 strain is stored in PDA medium, and freezing buffer is added;

[0048](2) Into the sterilized and cooled 50ml PDA medium, insert the bacterium ring obtained in step (1), shake the flask at a rotating speed of 200rpm at 30°C for 24 hours; after cultivating the 50ml shake flask, inoculate the culture medium Add 5L of flax, jute or kenaf fermentation medium, and shake the flask for 24 hours under the conditions of a rotating speed of 200r / min and a temperature of 30°C to obtain a fermentation broth;

[0049] (3) Centrifuge the fermentation broth obtained in step (3) at 4° C. at 8,000 r / min, and take the supernatant, which is laccase. Then determined by spectrophotometry, the laccase activity is 10-30IU / ml, and the enzyme activity is relatively stable at pH 5.0-6.0 and 20-60°C.

[0050] Enzyme activity assay method, using guaiacol as laccase substrate, and meas...

Embodiment 3

[0052] The retting process of flax, jute or kenaf is as follows:

[0053] (1) Preserve Candida tropicalis DK2 strain in PDA medium (per 1000ml medium includes 200g potato, 20g glucose, 15g agar, 1000mL distilled water) and add freezing buffer. The formula of every 100ml of freezing buffer of described step: potassium dihydrogen phosphate 0.1g, dipotassium phosphate 0.02g, sodium citrate 0.06g, magnesium sulfate heptahydrate 0.03g, glycerin 12ml, add water and be settled to 100ml, prepare have to.

[0054] (2) Insert a ring of bacteria into 1000ml of flax, jute or kenaf bran medium (per 1000ml medium includes 200g of flax, jute or kenaf bran, 50g of bran, 50ml of wort and 950ml of water), shake the flask Culture, the culture condition is 30°C, the pH is natural, and the culture time is 24 hours;

[0055] (3) Inoculate the culture medium into flax, jute or kenaf bran fermentation medium (every 1000ml culture medium includes flax, jute or kenaf bran 100g, bran 100g, wort juice ...

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Abstract

The invention relates to a Candida tropicalis DK2 strain and application thereof, the ITS sequence of which is shown in SEQ ID NO:1. The strain is used in retting to prepare flax, jute, kenaf or bamboo fiber, scouring flax, jute, kenaf or bamboo yarn and degumming flax, jute, kenaf or bamboo fabric. The bacterial strain of the present invention has the advantages of short growth period, strains that are not easy to be polluted, low processing cost, good fiber quality after processing, mild processing environment, good heat resistance, and no environmental pollution; it can be directly used in flax, jute, kenaf and bamboo Fiber degumming, with short degumming cycle, high fiber dispersion rate and high degumming efficiency.

Description

technical field [0001] The invention belongs to the technical field of biological textiles, and in particular relates to a Candida tropicalis DK2 strain and an application thereof. Background technique [0002] Saccharomyces pseudodeath is a rot parasite that widely exists in nature and can be isolated from fruits, vegetables, dairy products, and soil. [0003] In the primary processing of hemp, it is necessary to pre-treat part of the gum (mainly pectin and hemicellulose) through a retting process. The retting process actually uses traditional microbial technology, which is a process of slowly removing non-cellulose gum attached to the fiber surface through the synergistic action of microorganisms and enzymes. The retting process has a great influence on the quality of hemp fiber, and the commonly used retting methods include rain retting and enzymatic retting. [0004] In addition, in textile processing, it is necessary to go through further cooking and degumming, that i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16D01C1/00C12R1/74
CPCC12N1/165C12R2001/74D01C1/04D10B2201/01
Inventor 丁若垚郁崇文张兴群郭营李晓龙关赛鹏
Owner DONGHUA UNIV
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