Salt-tolerant protease-resistant α-galactosidase agaahj8 and its gene

A galactosidase and protease technology, applied in the field of genetic engineering, can solve the problems of low equipment utilization rate, raw material utilization rate, low amino nitrogen yield, long fermentation cycle, etc., and achieve the effect of good salt resistance and protease resistance characteristics.
CN104726430BActive Publication Date: 2018-01-19YUNNAN NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YUNNAN NORMAL UNIV
Publication Date
2018-01-19

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Abstract

The invention discloses a salt-resistant proteinase-resistant alpha-galactosidase AgaAHJ8 and a gene thereof. The invention provides an alpha-galactosidase AgaAHJ8 of which the amino acid sequence is disclosed as SEQ ID NO.1, a gene agaAHJ8 for coding the alpha-galactosidase, a recombinant vector comprising the gene, and a recombinant strain comprising the gene. The alpha-galactosidase can maintain the enzyme activity at 72% above when the pH value is 4.0-8.0. The optimum temperature is 50 DEG C, and the alpha-galactosidase is stable at 37 DEG C. The alpha-galactosidase has favorable salt resistance and proteinase resistance, and can hydrolyze melibiose, raffinose and guar gum. The alpha-galactosidase AgaAHJ8 is applicable to the field of processing of high-salt food and marine products, and is especially applicable to fermentation of high-salt food (such as high-salt diluted soy sauce).
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Description

technical field

[0001] The invention relates to the technical field of genetic engineering, in particular to a salt-resistant and protease-resistant α-galactosidase AgaAHJ8 and its gene. Background technique

[0002] Oligosaccharides and polysaccharides with α-galactoside are widely found in food and feed materials, especially legume seeds have the highest content (Karr-Lilienthal et al., Livest Prod Sci, 2005, 97:1– 12.), such as oligosaccharides such as melibiose, raffinose, stachyose and verbascose, and galactomannans such as carob gum and guar gum. α-galactosidase (α-galactosidase, EC 3.2.1.22), also known as melibiase, can catalyze the hydrolysis of α-galactosidic bonds in these oligosaccharide and polysaccharide substrates, and then apply In feed, food, paper and medical industries (Zhou et al., Appl Microbiol Biotechnol, 2010, 88:1297–1309).

[0003] Salt-tolerant enzymes still have catalytic activity under high concentrations of NaCl, and can be used in high-salt f...

Claims

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