32 results about "Melibiose" patented technology

There are provided novel plant-derived enzymes for hydrolysis of sugars and particularly an alkaline alpha-galactosidase which hydrolyzes a broad spectrum of galactosyl-saccharides such as melibiose, raffinose and stachyose and guar gum, at neutral to alkaline pH conditions.

The invention relates to strains of S. cerevisiae capable of secreting Alpha-galactosidase into culture medium, as well as to methods for obtaining Alpha-galactosidase using said strains and methods for producing biomass and bioethanol by means of culturing said strains in media rich in galactose. In another aspect, the invention provides methods for expressing recombinant proteins using a composition rich in galactose as a culture medium for the microorganisms producing said protein.

The invention provides a mutant enzyme having trans-sialidase activity (EC 3.2.1.18), characterized by an enhanced trans-sialidase:sialidase ratio when compared to its parent sialidase enzyme. Further the enzyme may be used in a method for trans-sialylating mono- and oligo-saccharides, including galacto-oligosaccharides (GOS), fructo-oligosaccharides (FOS), malto-oligosaccharides (MOS), isomalto-oligosaccarides (IMO), lactulose, melibiose, maltose, glycosyl sucrose, lactosucrose and fucose. Trans-sialidated mono- and oligo-saccharides, produced with the mutant enzyme, are useful in preparing infant formula, a prebiotic nutritional supplement, and a food supplement.

The purpose of the present invention is to provide the following: a dried influenza vaccine preparation with which stable retention of the activity of influenza vaccine antigen is possible, even when the preparation is stored without strict low-temperature management, and with which a stable supply is possible; and a method for producing the dried influenza vaccine preparation. The present invention provides a dried influenza vaccine preparation comprising influenza vaccine antigen and a disaccharide, wherein the disaccharide is one or more selected from the group consisting of sucrose, maltose, palatinose, melibiose, isomalt, cellobiose, allolactose, isomaltose, sophorose, lactobionic acid, laminaribiose, xylobiose, turanose, gentiobiose, rutinose, kojibiose, nigerose, robinose, neohesperidose, sucralose, and maltitol.

The invention discloses a salt-resistant proteinase-resistant alpha-galactosidase AgaAHJ8 and a gene thereof. The invention provides an alpha-galactosidase AgaAHJ8 of which the amino acid sequence is disclosed as SEQ ID NO.1, a gene agaAHJ8 for coding the alpha-galactosidase, a recombinant vector comprising the gene, and a recombinant strain comprising the gene. The alpha-galactosidase can maintain the enzyme activity at 72% above when the pH value is 4.0-8.0. The optimum temperature is 50 DEG C, and the alpha-galactosidase is stable at 37 DEG C. The alpha-galactosidase has favorable salt resistance and proteinase resistance, and can hydrolyze melibiose, raffinose and guar gum. The alpha-galactosidase AgaAHJ8 is applicable to the field of processing of high-salt food and marine products, and is especially applicable to fermentation of high-salt food (such as high-salt diluted soy sauce).

New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.

New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.

The invention discloses a simultaneous rapid detection method for various sugars, sugar alcohols and alcohols in beer, which belongs to the field of analytical chemistry. The invention adopts integral pulse amperometry-ion chromatography and optimizes the pre-treatment detection method to study the influencing components Separation of experimental factors, establishment of one-time simultaneous detection of 23 kinds of sugars, sugar alcohols, alcohols (2,3-butanediol, ethanol, methanol, glycerol, erythritol, xylitol, fucoidin Sugar, arabitol, sorbitol, trehalose, ribitol, mannitol, 2‑deoxy‑D‑glucose, mannose, melibiose, glucose, maltitol, fructose, lactose, ribose, sucrose, raffinose, Maltose) method solves the technical problem of accurate analysis of multiple components of sugars, sugar alcohols and alcohols in beer. This method is fast, accurate and sensitive, and realizes simultaneous analysis of multiple categories and components. make an important contribution.

There are provided novel plant-derived enzymes for hydrolysis of sugars and particularly an alkaline alpha-galactosidase which hydrolyzes a broad spectrum of galactosyl-saccharides such as melibiose, raffinose and stachyose and guar gum, at neutral to alkaline pH conditions.

The invention provides a sugar-reducing treatment method for a jujube extracting solution. According to the sugar-reducing treatment method, in accordance with the nutrient components of the jujube extracting solution and the metabolic characteristic of bacterial strains, candida utilis is selected as a fermentation bacterial strain. Because of the characteristics that the candida utilis is low in requirement for growth factors, and can realize selective metabolism of glucose, cane sugar and raffinose, maltose, galactose, lactose and melibiose are not fermented, besides, the fat and the like are not decomposed, and based on reservation of most of nutrient components of the jujube extracting solution, sugar can be degrated in a pointed manner. Based on this, in combination with a biocatalysis technique, DPE epimerase is used for treating the jujube extraction solution, and conversion of the glucose and levulose into low-calorie low-glycemic-index functional rare sugar is facilitated. According to the method, the glucose content in the jujube extracting solution can be reduced by nearly 70%, the levulose content is reduced by more than 40%, and before and after treatment, the cAMP content is hardly reduced. Obtained products also reserve original flavor characteristics of the jujube extracting solution.

The invention discloses a method for simultaneous detection of 22 kinds of sugars, sugar alcohols and alcohols in fruit juice, which belongs to the field of analytical chemistry. The invention adopts integral pulse amperometric-ion chromatography and optimizes the pretreatment method to study the factors that affect the separation of components. Experimental factors, established a one-time simultaneous detection of 22 kinds of sugars, sugar alcohols, alcohols (2,3-butanediol, propylene glycol, methanol, glycerol, erythritol, xylitol, rhamnose , arabitol, sorbitol, trehalose, galactitol, mannitol, 2-deoxy-D-glucose, arabinose, melibiose, glucose, galactose, fructose, ribose, sucrose, raffinose, maltose) The method solves the technical problem of accurate analysis of multiple components of sugars, sugar alcohols and alcohols in fruit juice. This method is fast, accurate and sensitive, and realizes the simultaneous analysis of multiple categories and components. important contribution.

The present invention pertains to a method for producing a recombinant protein, said method comprising: a first step for culturing a recombinant cell, which expresses the recombinant protein under the control of an inducible promoter, in a medium containing galactose and at least two substances selected from the group consisting of mannitol, raffinose and melibiose; and a second step for, after the first step, further culturing the recombinant cell under such conditions that the expression from the inducible promoter is induced.