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32 results about "Melibiose" patented technology
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Melibiose is a reducing disaccharide formed by an α-1,6 linkage between galactose and glucose (D-Gal-α(1→6)-D-Glc). It differs from lactose in the chirality of the carbon where the galactose ring is closed and that the galactose is linked to a different point on the glucose moiety. It can be formed by invertase-mediated hydrolysis of raffinose, which produces melibiose and fructose. Melibiose can be broken down into its component saccharides, glucose and galactose, by the enzyme alpha-galactosidase, such as MEL1 from Saccharomyces pastorianus (lager yeast).
Plant-derived alkaline alpha-galactosidase
InactiveUS20070036883A1Hydrolyze raffinosePromote crystallizationMilk preparationPeptide/protein ingredientsLactoseSugar
There are provided novel plant-derived enzymes for hydrolysis of sugars and particularly an alkaline alpha-galactosidase which hydrolyzes a broad spectrum of galactosyl-saccharides such as melibiose, raffinose and stachyose and guar gum, at neutral to alkaline pH conditions.
Owner:STATE OF ISRAEL MINIST OF AGRI & RURAL DEV AGRI RES ORG (A R O) (VOLCANI CENT)
Transglycosyl alpha-galactoglucosidezyme gene
InactiveCN1778928ASolving difficult-to-grow problemsHigh synthesis efficiencyEnzymesFermentationGene expressionOligosaccharide
The invention presents a transfer glycation alpha-D-galactosides enzyme gene, this gene is gained with the PCR amplified of Bifidobacteriumbreve gene group DNA to get the high conservative DNA fragments and crossbreeds with Southern. The full length of its nucleotide sequence is 2226 base, and codes 741 amino acids. The attribute of the recombination enzyme ,which is expressed by this gene, is that the enzyme is double-subunit albumen, the molecular weight of polymer is about 160kD, the molecular weight of sigle-subunit is about 80kD, the Km of this enzyme to nitrophenol alpha-D-galactosides is 0.17mmol / L, Vmax is 4.43 mol / (Líñmin); the best reaction temperature is 37 deg.C, and the best PH is 5.5-6.0, the enzyme has transfer glycation activity in the reaction system of hydrolysis melibinose, or raffinose, this reaction can create transfer glycation production; the different of the enzymeí»s glycosides substrate can be used to compound many glycoconjugate of a-galactosides oligosaccharide and alpha-D-galactosides. The gains of the enzyme can be used to get alpha-galactosides compound enyzme by gene alteration, and improve the compound efficiency of alpha-galactosides oligosaccharide with glycoconjugate radically.
Owner:SHANDONG UNIV
Saccharomyces cerevisiae capable of being co-fermented by a plurality of carbon sources and application thereof
The invention discloses saccharomyces cerevisiae capable of being co-fermented by a plurality of carbon sources and an application thereof, belonging to the technical fields of microbes and fermentation engineering. The saccharomyces cerevisiae namely a CCTCC M 2014463 strain is separated from Chinese Maotai-flavor liquor wine brewing fermented grains. The strain can adopt glucose, galactose, xylose, allulose, cane sugar, maltose, melibiose, turanose, mycose and melitose, and can adopt glucose and other sugars synchronously. Meanwhile, the saccharomyces cerevisiae can be used for resisting high temperature, high ethanol and high acid, and also can be used for producing acids, alcohols, esters and various flavor substances such as benzeneacetaldehyde, acetophenone 4-vinyl guaiacol and the like. The saccharomyces cerevisiae disclosed by the invention can be directly applied to the production of beverage wines, the reinforcement of wine brewing fermented grains in beverage wine production, and the production of fuel ethanol which is used as a carbon source substrate after starch and cellulose saccharification, thereby significantly improving the carbon source utilization rate and the ethanol yield.
Owner:JIANGNAN UNIV
Recombination food-grade lactic acid bacterium capable of being used for preventing or treating human ulcerative colitis as well as preparation method and application of recombination food-grade lactic acid bacterium
The invention relates to a recombination food-grade lactic acid bacterium for secreting and expressing a fusion protein containing a human acidic fibroblast growth factor, a preparation method of the recombination food-grade lactic acid bacterium, and in particular relates to a method for carrying out fusion expression by using a Usp45 protein signal peptide, a secretion-promoting protein peptide and the human acidic fibroblast growth factor. According to the invention, genes for coding the fusion proteins are cloned to a lactic acid bacterium constitutive expression vector pMG36m, then transformed into lactococcus lactis NZ9000, and subjected to recombinant screening by using melibiose as a carbon source. The recombination lactic acid engineering bacterium can continuously secret the fusion protein containing the human acidic fibroblast growth factor in a fermentation expression process. The invention relates to an application of the recombination food-grade lactic acid bacterium in the preparation of cosmetics and medicines or foods for preventing and treating human ulcerative colitis.
Owner:JINAN UNIVERSITY +1
Enzyme solution with better enzyme activity ratio of beta-mannase to alpha-galactosidase as well as preparation method and application of enzyme solution
PendingCN112760311AΒ-mannanase excellentExcellent activityFungiMicroorganism based processesΒ mannanaseMicrobial culture technique
The invention discloses an enzyme solution with a better enzyme activity ratio of beta-mannase to alpha-galactosidase as well as a preparation method and application of the enzyme solution, and belongs to the technical field of microbial culture. According to the preparation method, trichoderma reesei is taken as an enzyme-producing strain, microcrystalline cellulose and / or melibiose is taken as a carbon source, the enzyme solution is produced by adopting a fermentation method, the enzyme activity of the beta-mannase in the enzyme solution is not higher than 0.05 U / mL, and the enzyme solution has a better enzyme activity ratio of beta-mannase to the alpha-galactosidase. The enzyme solution can directly hydrolyze the galactomannan to prepare micromolecular galactomannan and galactomannan-oligosaccharide without purification, the yield of the micromolecular galactomannan and the galactomannan-oligosaccharide can be effectively increased, the production cost is reduced, and the enzyme solution has a very good application prospect.
Owner:NANJING FORESTRY UNIV
Strains of s.cerevisiae, capable of growing in media with melibiose, stachyose and raffinose
Owner:UNIV DA CORUNA
Method for simultaneous rapid detection of various sugars, sugar alcohols and alcohols in beer
ActiveCN110514777AImprove detection efficiencyReduce the cost of testing manpower and material resourcesComponent separationIon chromatographyArabitol
Owner:日照海关综合技术服务中心
Method for simultaneously detecting 22 sugars, sugar alcohol and alcohol in fruit juice
ActiveCN110514778ASolve the technical problems of low efficiency in component determinationSolve low-efficiency technical problemsComponent separationIon chromatographyArabitol
The invention discloses a method for simultaneously detecting 22 sugars, sugar alcohol and alcohol in fruit juice, and belongs to the field of analytical chemistry. The method adopts an integral impulse ampere-ion chromatography to research experiment factors affecting component separation via a processing method before optimization; and the method for simultaneously detecting the 22 sugars, the sugar alcohol and the alcohol (2,3-butanediol, propylene glycol, methyl alcohol, glycerin, erythritol, xylitol, rhamnose, arabitol, sorbitol, trehalose, galactitol, mannitol, 2-deoxidized-D-glucose, arabinose, melibiose, glucose, galactose, fructose, ribose, saccharose, raffinose and maltose) at a time is built. The technical difficulty of accurately analyzing the plurality of components, sugars, sugar alcohol and alcohol, in the fruit juice is solved; the method is fast, accurate and sensitive, achieves simultaneous analysis of the plurality of categories and the plurality of components, and makes an important contribution to component detection of the fruit juice.
Owner:日照海关综合技术服务中心
Dried influenza vaccine preparation and method of producing the same
InactiveUS20160206728A1Improve distributionEasy to storeSsRNA viruses negative-senseOrganic active ingredientsAntigenMaltitol
The present invention provides a dried influenza vaccine preparation in which the activity of an influenza vaccine antigen can be stably maintained even when stored without strictly maintaining a low temperature, and which can be stably supplied. The present invention also provides a method of producing the dried influenza vaccine preparation. The present invention provides a dried influenza vaccine preparation containing an influenza vaccine antigen and a disaccharide, wherein the disaccharide is at least one selected from the group consisting of sucrose, maltose, palatinose, melibiose, isomalt, cellobiose, allolactose, isomaltose, sophorose, lactobionic acid, laminaribiose, xylobiose, turanose, gentiobiose, rutinose, kojibiose, nigerose, robinose, neohesperidose, sucralose, and maltitol.
Owner:NITTO DENKO CORP
A mutant sialidase having trans-sialidase activity for use in production of sialylated glycans
ActiveUS20160076013A1Reduced hydrolase activityOrganic active ingredientsBiocideGalactooligosaccharideOligosaccharide
The invention provides a mutant enzyme having trans-sialidase activity (EC 3.2.1.18), characterized by an enhanced trans-sialidase:sialidase ratio when compared to its parent sialidase enzyme. Further the enzyme may be used in a method for trans-sialylating mono- and oligo-saccharides, including galacto-oligosaccharides (GOS), fructo-oligosaccharides (FOS), malto-oligosaccharides (MOS), isomalto-oligosaccarides (IMO), lactulose, melibiose, maltose, glycosyl sucrose, lactosucrose and fucose. Trans-sialidated mono- and oligo-saccharides, produced with the mutant enzyme, are useful in preparing infant formula, a prebiotic nutritional supplement, and a food supplement.
Owner:DAIRY CREST
Preparation method of oligosaccharide for promoting proliferation of intestinal probiotics
ActiveCN108384821ASimple production processLow costSugar derivativesOligosaccharidesFiberGalactooligosaccharide
The invention discloses a preparation method of oligosaccharide for promoting the proliferation of intestinal probiotics. According to the method, based on the selection specificity of enzymes on different substrates, lactose, cellobiose, melibiose, trehalose and other general disaccharide oligomers are respectively used as mixed substrates, a transglycosylation reaction is catalyzed with beta-galactosidase derived from Lactobacillus plantarum 70810, and the obtained products are subjected to classification purification through active carbon beta-diatomaceous earth adsorption chromatography soas to obtain various iso galactooligosaccharide mixtures. According to the present invention, the preparation method has characteristics of simple process and low cost; and the content of the oligosaccharides comprising more than three molecules in the oligosaccharides prepared by using the method is more than 95%; and the prepared oligosaccharide provides the significant proliferation activity to intestinal probiotics, can inhibit the growth of harmful bacteria, contains almost no monosaccharides and disaccharides, has the expanded application population and the expanded application field, has high safety, and can be directly added to various foods.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Mutant sialidase having trans-sialidase activity for use in production of sialylated glycans
ActiveUS10081801B2Carbohydrate active ingredientsChemical recyclingGalactooligosaccharideOligosaccharide
Owner:DAIRY CREST
A strain of Saccharomyces cerevisiae capable of co-fermentation with multiple carbon sources and its application
The invention discloses a strain of Saccharomyces cerevisiae capable of co-fermenting with multiple carbon sources and an application thereof, belonging to the technical field of microorganisms and the technical field of fermentation engineering. The Saccharomyces cerevisiae CCTCC M 2014463 strain of the present invention is isolated from the fermented grains of Chinese Maotai-flavor liquor. The strain can utilize glucose, galactose, xylose and allulose, sucrose, maltose, melibiose, turanose, trehalose, raffinose, and can simultaneously utilize glucose and other sugars above. At the same time, the yeast can tolerate high temperature, high ethanol and high acid, and produce acids, alcohols, esters, phenylacetaldehyde, acetophenone 4-vinylguaiacol and other flavor substances. The yeast can be directly used in the production of drinking wine, the strengthening of Saccharomyces cerevisiae in the production of drinking wine, and the production of fuel ethanol as a carbon source substrate after saccharification of starch and cellulose, and can significantly improve the utilization rate of carbon source and the yield of ethanol.
Owner:JIANGNAN UNIV
Method for transferring a glucosyl residue
An object of the present invention is to provide novel methods for forming glucosyl-transferred polyalcohols, glucosyl-transferred glucuronic acid, and glucosyl-transferred derivatives of glucose whose C-6 hydroxyl group bound to a saccharide by using an enzymatic reaction. The present invention solves the above object by providing a method for transferring a glucosyl residue to polyalcohols, glucuronic acid and / or derivatives of glucose whose C-6 hydroxyl group bound to a saccharide, comprising a step of: allowing a trehalose phosphorylase to act on a saccharide containing glucose as a component sugar and one or more polyalcohols selected from the group consisting of inositol, ribitol, erythritol, and glycerol; glucuronic acid and / or a salt thereof; and / or one or more derivatives of glucose whose C-6 hydroxyl group bound to a saccharide selected from the group consisting of isomaltose, gentiobiose, melibiose, isomaltotriose, and isopanose.
Owner:HAYASHIBARA CO LTD
Dried influenza vaccine preparation and method for producing dried influenza vaccine preparation
InactiveCN105530955AMaintain activity stablyStable supplySsRNA viruses negative-sensePowder deliveryAntigenMaltitol
Owner:NITTO DENKO CORP
Mono- and disaccharide derivatives
InactiveUS8052981B2Easy to useImprove bioavailabilityEsterified saccharide compoundsAntibacterial agentsAdjuvantMaltose
The present invention includes a formulation comprising a physiological salt solution, or an oil-in-water emulsion, or a water immiscible solid phase, and an adjuvant comprising one or more disaccharide derivatives derived from cellobiose, gentiobiose, lactose, lactulose, maltose, melibiose, sucrose or turanose, wherein at least four of the free hydroxyl groups of the disaccharide molecule have been modified such that the disaccharide derivative has: (i) at least 3, but not more than N-1, fatty acid ester groups, wherein each of the fatty acid ester groups is represented by a general formula of —O—C(═O)—(CH2)xCH3 wherein x is between 6 and 14, and (ii) at least one, but no more than N-1 anionic groups, wherein N is the number of hydroxyl groups of the disaccharide from which the derivative is derived and wherein the combined number of fatty acid esters and anionic groups does not exceed N.
Owner:BTG INT LTD
Salt-tolerant protease-resistant α-galactosidase agaahj8 and its gene
ActiveCN104726430BImprove salt toleranceImprove featuresBacteriaFermentationProtease resistantGuar gum
The invention discloses a salt-resistant proteinase-resistant alpha-galactosidase AgaAHJ8 and a gene thereof. The invention provides an alpha-galactosidase AgaAHJ8 of which the amino acid sequence is disclosed as SEQ ID NO.1, a gene agaAHJ8 for coding the alpha-galactosidase, a recombinant vector comprising the gene, and a recombinant strain comprising the gene. The alpha-galactosidase can maintain the enzyme activity at 72% above when the pH value is 4.0-8.0. The optimum temperature is 50 DEG C, and the alpha-galactosidase is stable at 37 DEG C. The alpha-galactosidase has favorable salt resistance and proteinase resistance, and can hydrolyze melibiose, raffinose and guar gum. The alpha-galactosidase AgaAHJ8 is applicable to the field of processing of high-salt food and marine products, and is especially applicable to fermentation of high-salt food (such as high-salt diluted soy sauce).
Owner:YUNNAN NORMAL UNIV
Sugar-reducing treatment method for jujube extracting solution
ActiveCN106879991ALower glucose levelsPreserve the flavor profileFood scienceLow calorieBacterial strain
The invention provides a sugar-reducing treatment method for a jujube extracting solution. According to the sugar-reducing treatment method, in accordance with the nutrient components of the jujube extracting solution and the metabolic characteristic of bacterial strains, candida utilis is selected as a fermentation bacterial strain. Because of the characteristics that the candida utilis is low in requirement for growth factors, and can realize selective metabolism of glucose, cane sugar and raffinose, maltose, galactose, lactose and melibiose are not fermented, besides, the fat and the like are not decomposed, and based on reservation of most of nutrient components of the jujube extracting solution, sugar can be degrated in a pointed manner. Based on this, in combination with a biocatalysis technique, DPE epimerase is used for treating the jujube extraction solution, and conversion of the glucose and levulose into low-calorie low-glycemic-index functional rare sugar is facilitated. According to the method, the glucose content in the jujube extracting solution can be reduced by nearly 70%, the levulose content is reduced by more than 40%, and before and after treatment, the cAMP content is hardly reduced. Obtained products also reserve original flavor characteristics of the jujube extracting solution.
Owner:CANGZHOU ENJI BIOLOGICAL PROD +1
Melibiose Operon Expression System
New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.
Owner:LONZA AG
Melibiose operon expression system
Owner:MORFOZIS AG
Saccharide composition, glycosyl composition and cryopreservation liquid
The invention discloses a saccharide composition. The saccharide composition comprises at least one of trehalose, cane sugar, melibiose and mannan. The invention further provides a glycosyl composition, a cryopreservation solution and application of the cryopreservation solution. According to the freezing medium provided by the invention, low-dose DMSO and dextran 40 are selected, a combination of trehalose, cane sugar, melibiose and mannan is used for replacing fetal calf serum (FBS) to be used as a basic formula of the freezing medium for composition, and inhibitors of vitamin C, resveratrol, taurine, L-arginine and Caspase9 are added, so that the freezing medium is prepared. And the cells subjected to low-temperature cryopreservation can still keep good cell activity after being recovered according to a specific program. The pollution of pathogenic bacteria can be reduced, and the safety is higher; meanwhile, the doping of heterologous protein is avoided, the use amount of DMSO is reduced, and the clinical use risk can be reduced.
Owner:EDIGENE GUANGZHOU INC
Strains of s.cerevisiae capable of growing in media with melibiose, stachyose and raffinose
The invention relates to strains of S. cerevisiae capable of secreting α-galactosidase into culture medium, as well as to methods for obtaining α-galactosidase using said strains and methods for producing biomass and bioethanol by means of culturing said strains in media rich in galactose. In another aspect, the invention provides methods for expressing recombinant proteins using a composition rich in galactose as a culture medium for the microorganisms producing said protein.
Owner:UNIV DA CORUNA
Melibiose operon expression system
New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.
Owner:MORFOZIS AG
Simultaneous rapid detection method for multiple sugars, sugar alcohols and alcohols in beer
ActiveCN110514777BImprove detection efficiencyReduce the cost of testing manpower and material resourcesComponent separationIon chromatographyD-arabitol
Owner:日照海关综合技术服务中心
Plant-derived alkaline alpha-galactosidase
InactiveUS7514254B2Hydrolyze raffinosePromote crystallizationMilk preparationPeptide/protein ingredientsSugarHydrolysis
Owner:STATE OF ISRAEL MINIST OF AGRI & RURAL DEV AGRI RES ORG (A R O) (VOLCANI CENT)
Recombinant food lactic acid bacteria for preventing chronic heavy metal poisioning and preparation method and use thereof
The invention relates to recombinant food lactic acid bacteria containing human IA type metallothionein fusion protein and a preparation method thereof, in particular to a method for performing fusion expression by using glutathione S-transferase, small ubiquitin-related modifier mature peptide and human IA type metallothionin. In the invention, the gene of the fusion protein is cloned into a lactic acid bacterium-based food expression vector pMG36m and then transferred to the lactococcus lactis MG1363, and then melibiose is used as a carbon source to perform recombinant screening. The recombinant lactic acid engineering bacteria can continuously express the fusion protein of the human IA type metallothionin in a fermentation process, and the protein is capable of bonding heavy metal Pb, Cd and Hg in living environments. The invention also relates to the use of the recombinant food lactic acid bacteria in preparation of medicines, foods or cosmetics for preventing human chronic metal poisioning.
Owner:广州市暨源生物科技有限公司 +1
A hypoglycemic treatment method for jujube extract
ActiveCN106879991BLower glucose levelsPreserve the flavor profileFood scienceBiotechnologyGlycaemic index
The invention provides a sugar-reducing treatment method for a jujube extracting solution. According to the sugar-reducing treatment method, in accordance with the nutrient components of the jujube extracting solution and the metabolic characteristic of bacterial strains, candida utilis is selected as a fermentation bacterial strain. Because of the characteristics that the candida utilis is low in requirement for growth factors, and can realize selective metabolism of glucose, cane sugar and raffinose, maltose, galactose, lactose and melibiose are not fermented, besides, the fat and the like are not decomposed, and based on reservation of most of nutrient components of the jujube extracting solution, sugar can be degrated in a pointed manner. Based on this, in combination with a biocatalysis technique, DPE epimerase is used for treating the jujube extraction solution, and conversion of the glucose and levulose into low-calorie low-glycemic-index functional rare sugar is facilitated. According to the method, the glucose content in the jujube extracting solution can be reduced by nearly 70%, the levulose content is reduced by more than 40%, and before and after treatment, the cAMP content is hardly reduced. Obtained products also reserve original flavor characteristics of the jujube extracting solution.
Owner:CANGZHOU ENJI BIOLOGICAL PROD +1
A method for simultaneous detection of 22 kinds of sugars, sugar alcohols and alcohols in fruit juice
ActiveCN110514778BSolve the technical problems of low efficiency in component determinationSolve low-efficiency technical problemsComponent separationIon chromatographyArabitol
Owner:日照海关综合技术服务中心
Rhizospheric prebiotics composition for reducing incidence rate of tomato bacterial wilt and application of composition
The invention discloses a rhizospheric prebiotics composition for reducing the incidence rate of tomato bacterial wilt and an application of the composition. The soil microbial prebiotics compositioncomprises a carbohydrate substance or a carbohydrate substance, an ester substance and an alcohol substance, the carbohydrate substance is selected from any one or more of melibiose, fructose, sucrose, ribose, xylose, mannose and maltose, the ester substance is selected from gluconolactone, and the alcohol substance is selected from any one or two of inositol and ribtol adonite. The prebiotics composition is applied, total bacteria abundance in rhizospheric soil can be improved, proliferation of ralstonia solanaceara is restrained, so that the incidence rate of bacterial wilt is remarkably reduced, and the rhizospheric prebiotics composition is suitable for being applied to crops easily subjected to the bacterial wilt.
Owner:NANJING AGRICULTURAL UNIVERSITY
Method for producing recombinant protein
The present invention pertains to a method for producing a recombinant protein, said method comprising: a first step for culturing a recombinant cell, which expresses the recombinant protein under the control of an inducible promoter, in a medium containing galactose and at least two substances selected from the group consisting of mannitol, raffinose and melibiose; and a second step for, after the first step, further culturing the recombinant cell under such conditions that the expression from the inducible promoter is induced.
Owner:SPIBER INC
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