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Simultaneous rapid detection method for multiple sugars, sugar alcohols and alcohols in beer

A technology for beer and sugar alcohol, applied in the field of analytical chemistry, can solve the problems of inability to detect multiple components at the same time, unsuitable for accurate quantitative analysis, small number of components, etc., achieve good economic benefits and social application value, and reduce testing manpower and material resources. Cost, effect of improved accuracy and precision

Active Publication Date: 2021-06-11
日照海关综合技术服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liquid chromatography is convenient and versatile, but its sensitivity is low, and it is difficult to detect samples with complex matrices
Gas chromatography has high sensitivity, but it needs derivation, and the quantitative repeatability is poor, so it is not suitable for accurate quantitative analysis
Enzyme electrode method generally only detects one kind of sugar, which has good specificity, but it cannot detect multiple components at the same time
[0004] At present, although people have studied the methods for the simultaneous detection of multiple sugars, sugar alcohols and alcohols in beer, what can be retrieved in the current database is only for a certain type of components (such as simple sugars or Alcohols) or the number of components detected at the same time is small (only about 10 kinds of sugars and sugar alcohols are analyzed simultaneously), which cannot meet the growing detection needs. Therefore, research and establish a method with strong applicability, good selectivity, A method for simultaneous detection of multiple sugars, sugar alcohols and alcohols with high sensitivity has important practical significance and application value

Method used

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  • Simultaneous rapid detection method for multiple sugars, sugar alcohols and alcohols in beer
  • Simultaneous rapid detection method for multiple sugars, sugar alcohols and alcohols in beer
  • Simultaneous rapid detection method for multiple sugars, sugar alcohols and alcohols in beer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: a method for simultaneous detection of 23 kinds of sugars, sugar alcohols and alcohols in beer

[0047] 1. Instruments and Reagents

[0048] Ion chromatograph (Thermo Scientific ICS5000+), equipped with electrochemical detector and automatic sample injector (AS-SP); Milli-Q ultrapure water instrument (Millipore, USA); intelligent ultrasonic cleaner (Shanghai Zhixin Instrument Co., Ltd. DL-360E); electronic balance (Mettler ML802 / 02); experimental water: ultrapure water (resistivity 18.2MΩ.cm); sugar, sugar alcohol, alcohol standard or standard reagent (purity>98% ); sodium hydroxide solution (50% w / w, Fisher Chemical); hydrochloric acid (excellent grade); IC-RP10 column (Agela Technologies); IC-H10 column (Agela Technologies).

[0049] 2. Ion Chromatography Conditions

[0050] Chromatographic separation column: Dionex CarboPac TM MA1 (4×250 mm), guard column: Dionex CarboPac TM MA1 (4×50 mm); integrated pulse amperometric detection; working electrode: G...

Embodiment 2

[0058] Example 2: Influence of flow rate on detection

[0059] According to the conditions of the experimental analysis in Example 1, under the condition that other conditions remain unchanged, the flow rate of the eluent is successively tested to be 0.30 mL / min, 0.40 mL / min, 0.45 mL / min, and 0.50 mL / min. The component separation of the standard solution, the ion chromatograms of the mixed standard solution at different flow rates are as follows: figure 2 Shown; The relationship between the eluent flow rate and the system pressure is shown in Table 2.

[0060] Table 2 Relationship between eluent flow rate and system pressure

[0061]

[0062] Will figure 2 Combined with Table 2, it can be concluded that the greater the flow rate of the eluent, the greater the system pressure, and the faster the peak time of the components, the flow rate increased from 0.30 mL / min to 0.50 mL / min, and the total peak time of the 23 components From 90 minutes to 62 minutes gradually, while...

Embodiment 3

[0063] Example 3: Influence of multi-stage gradient elution conditions on detection

[0064] According to the conditions of the experimental analysis in Example 1, the separation of the mixed standard solution components was tested successively under the conditions of the initial eluent concentrations of 450, 470, 480, 490, and 500 mmol / L under the condition of other conditions unchanged. , the component separation of the mixed standard solution is as follows image 3 shown.

[0065] From image 3 It can be seen from the figure that when the initial eluting concentration is 450 mmol / L, the separation degree of components 7 and 8, components 19 and 20 is not high, and components 10 and 11 are almost overlapped; when the initial eluting concentration is 470 mmol / L , the resolution of components 10 and 11 was not high; when the initial eluting concentrations were 490 and 500 mmol / L, the resolution of components 14 and 15 decreased significantly, while the separation of componen...

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Abstract

The invention discloses a simultaneous rapid detection method for various sugars, sugar alcohols and alcohols in beer, which belongs to the field of analytical chemistry. The invention adopts integral pulse amperometry-ion chromatography and optimizes the pre-treatment detection method to study the influencing components Separation of experimental factors, establishment of one-time simultaneous detection of 23 kinds of sugars, sugar alcohols, alcohols (2,3-butanediol, ethanol, methanol, glycerol, erythritol, xylitol, fucoidin Sugar, arabitol, sorbitol, trehalose, ribitol, mannitol, 2‑deoxy‑D‑glucose, mannose, melibiose, glucose, maltitol, fructose, lactose, ribose, sucrose, raffinose, Maltose) method solves the technical problem of accurate analysis of multiple components of sugars, sugar alcohols and alcohols in beer. This method is fast, accurate and sensitive, and realizes simultaneous analysis of multiple categories and components. make an important contribution.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a simultaneous rapid detection method for multiple sugars, sugar alcohols and alcohols in beer. Background technique [0002] Beer is an alcoholic beverage brewed from malt and hops through gelatinization, saccharification, and liquid fermentation. Beer contains a variety of vitamins, amino acids, sugars, sugar alcohols and other ingredients. Carbohydrates are a class of polyhydroxy aldehydes or ketones and their derivatives. They are energy substances for human life activities and important components of many foods and beverages. Sugar alcohols are the reduced aldehyde or ketone groups of sugars. They are usually sweeteners produced by catalytic hydrogenation of corresponding sugars. They have the advantages of preventing diabetes, low calories, and good taste. Alcohols are important components in some fermented foods. Some alcohols are beneficial to human health...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/96G01N30/88G01N30/06G01N30/14G01N30/34G01N30/30
CPCG01N30/06G01N30/14G01N30/30G01N30/34G01N30/88G01N30/96G01N2030/047G01N2030/146G01N2030/884
Inventor 宋卫得苏征高尧华王凯张传杰尹相英
Owner 日照海关综合技术服务中心
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