Recombinant food lactic acid bacteria for preventing chronic heavy metal poisioning and preparation method and use thereof

A lactic acid bacteria, food-grade technology, applied in the field of genetic engineering, can solve problems such as teratogenicity, hindering intestinal absorption of iron, and damaging blood vessels
CN102296046BActive Publication Date: 2013-08-14广州市暨源生物科技有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
广州市暨源生物科技有限公司
Publication Date
2013-08-14

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Abstract

The invention relates to recombinant food lactic acid bacteria containing human IA type metallothionein fusion protein and a preparation method thereof, in particular to a method for performing fusion expression by using glutathione S-transferase, small ubiquitin-related modifier mature peptide and human IA type metallothionin. In the invention, the gene of the fusion protein is cloned into a lactic acid bacterium-based food expression vector pMG36m and then transferred to the lactococcus lactis MG1363, and then melibiose is used as a carbon source to perform recombinant screening. The recombinant lactic acid engineering bacteria can continuously express the fusion protein of the human IA type metallothionin in a fermentation process, and the protein is capable of bonding heavy metal Pb, Cd and Hg in living environments. The invention also relates to the use of the recombinant food lactic acid bacteria in preparation of medicines, foods or cosmetics for preventing human chronic metal poisioning.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering. More specifically, the present invention relates to a recombinant food-grade lactic acid bacterium containing human type IA metallothionein fusion protein, its preparation method and the use of the recombinant food-grade lactic acid bacterium in the preparation of drugs, food or cosmetics for preventing chronic heavy metal poisoning in humans. in the application. Background technique

[0002] With the development of industrialization of various industries in China, heavy metal pollution has become a problem of great concern to the society. A large number of toxic heavy metal ions pollute through water, air, soil and the biological chain to animal and plant products and water sources on which human beings depend for survival, bringing potential harm to people's health. Generally speaking, heavy metal pollution refers to the pollution of heavy metal ions such as lead, mercury, cadmium, and arse...

Claims

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