Solid state fermentation belt reactor and application thereof

A solid-state fermentation and reactor technology, applied in solid-phase fermentation bioreactors, bioreactor/fermenter combinations, and specific-purpose bioreactors/fermenters, etc., can solve sampling difficulties, high labor intensity, solid materials Problems such as poor liquidity, to achieve the effect of reducing labor intensity, high automation intensity, and stable fermentation process

Active Publication Date: 2015-08-19
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the industrial-scale production of solid-state fermentation is almost at a standstill at present. The reasons are as follows: 1. The sterile environment is difficult to control, and the contamination rate of bacteria is high: most solid-state fermentation reactors combine the sterilization of materials with the fermentation process. Separate operation, and natural ventilation is used in the fermentation process, which is often labor-intensive, low in efficiency, and small in production capacity, and it is easy to cause bacterial contamination in the fermentation process
2. Feeding and discharging cannot be automated: Due to the characteristics of solid materials, general reactors can only feed

Method used

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  • Solid state fermentation belt reactor and application thereof
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  • Solid state fermentation belt reactor and application thereof

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Experimental program
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Effect test

Embodiment 1

[0044] Such as figure 1The solid-state fermentation belt reactor shown includes a fermenter 1, a medium preparation tank 10 and a conveyor belt system 11; wherein the fermenter 1 is a stainless steel horizontal fermenter, and the first support 3-1 at the bottom and the second The support 3-2 is fixed on the base 3. A jacket 4-1 is provided on the outer periphery of the fermenter, and the jacket 4-1 is provided with a heat exchange medium inlet 4-2 and a heat exchange medium outlet 4-3, and the heat exchange medium inlet 4-2 is arranged on the jacket 4 -1 is at the top, the heat exchange medium outlet 4-3 is located at the bottom of the jacket 4-1, and the heat exchange medium inlet 4-2 and the heat exchange medium outlet 4-3 are far apart in the horizontal direction. The heat exchange medium is water vapor and circulating cooling water.

[0045] A first movable top cover 2-1 and a second movable top cover 2-2 are arranged on both sides of the fermentation tank 1 in the horiz...

Embodiment 2

[0051] Embodiment 2: Aerobic microorganisms (using Bacillus subtilis solid-state fermentation to produce gamma-polyglutamic acid agricultural fertilizer as an example)

[0052] γ-polyglutamic acid (γ-polyglutamic acid, γ-PGA) is a type of homopolyamino acid synthesized by microorganisms, with a relative molecular mass of up to 200×10 4 Da. Due to the existence of a large number of hydrogen bonds and hydrophilic free carboxyl groups in its structure, it has excellent moisture retention properties and has been widely used in agricultural fertilizers.

[0053] The microorganism fermented in the present embodiment is the Bacillus subtilis NX-2 (bacteria strain preservation number: CGMCC 0833) that this laboratory has obtained authorization, directly uses microbial bacterial agent 5 * 10 10 cfu / g) inoculation, the inoculation amount is 25g / kg.

[0054] Solid fermentation medium: 600g / kg of mushroom residue after cultivation of shiitake mushrooms, 400g / kg of monosodium glutamate m...

Embodiment 3

[0059] Embodiment 3: Aerobic microorganism (taking solid-state fermentation Bacillus subtilis phosphate-solubilizing bacteria as an example)

[0060] Phosphorus is one of the essential nutrients for plant growth and development. Soil phosphorus deficiency directly affects the growth of crops and food production. In my country, the area of ​​phosphorus-deficient cultivated land reaches 74%, and more than 95% of the phosphorus in the soil is in an invalid state, which is difficult to be absorbed. Plants directly absorb and utilize. There are many phosphorus-solubilizing microorganisms in the soil, which can degrade these insoluble phosphates into free forms for plant absorption and utilization. Therefore, it is necessary to develop phosphorus-solubilizing microorganisms, reduce the production cost of phosphate fertilizers, solve the pollution of phosphate fertilizers and improve the utilization rate of phosphorus. Research is necessary.

[0061] The phosphate-solubilizing bacter...

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Abstract

The invention discloses a solid state fermentation belt reactor. The solid state fermentation belt reactor comprises a fermentation tank, a medium preparation tank and a conveyor belt system, the periphery of the fermentation tank is provided with a jacket, and the fermentation is also provided with a gas outlet, probes, a steam inlet, a steam outlet, a sewage outlet, movable top covers and a two-fluid nozzle device. The invention also discloses an application of the solid state fermentation belt reactor in cultivation of aerobic, anaerobic and facultative anaerobic microbes. The reactor has the advantages of high automation intensity, stable fermentation process, simple operation, realization of single-batch and multi-batch fermentation production, and facilitation of industrial application of solid state fermentation.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering and process engineering, and in particular relates to a solid-state fermentation belt reactor and its application. Background technique [0002] Solid-state fermentation refers to a biological reaction process with one or more microorganisms in a water-insoluble substrate with a certain humidity in the absence or almost no free water. Compared with liquid fermentation, it has many advantages, such as: simple medium and wide sources, less investment, low energy consumption, high product yield, simple post-extraction process, etc. In particular, crops or crop residues can be used as biological substrates to transform them into high-value products that are beneficial or harmless to people. It does not damage the natural ecosystem, but also solves the pollution problem and alleviates the resource crisis. Therefore, modern solid-state fermentation technology has received unprecedente...

Claims

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Application Information

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IPC IPC(8): C12M1/36C12M1/34C12M1/16C12M1/12C12M1/04C12M1/02C12M1/00
Inventor 冯小海徐虹汤宝许宗奇姜永翔梁金丰
Owner NANJING UNIV OF TECH
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