Preparation method of composite biological agent for restoring threonine fermentation waste liquid

A technology for fermentation waste liquid and biological preparations is applied in the field of preparation of composite biological preparations, which can solve the problems of many times of feeding biological preparations, unreasonable microbial compatibility, and difficulty in light transmission, so as to achieve the effect of promoting nitrification and denitrification and reduce sludge. Yield, ease of transportation and preservation

Inactive Publication Date: 2015-09-16
内蒙古阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no mature complete set of technology applied to production practice at home and abroad.
The main problem is that the one-time investment is too large, or the daily operation cost is too high, most manufacturers cannot bear it, and have to maintain the status quo of excessive emissions for a long time
The applicant's previous research was to use wastewater as a fertilizer, which is suitable for enterprises with fertilizer production qualifications and capabilities, but for other amino acid production enterprises, it cannot achieve the purpose of producing fertilizers
[0004] At present, the hot research is biological agents, which have the advantages of low cost and no secondary pollution, but have unreasonable microbial compatibility, low treatment efficiency, and microbial carriers have poor internal mass transfer, are not easy to transmit light, and have a specific surface area. Small size, short service life and other defects; biological agents need to be put in many times, which increases the cost, and the large amount of dosing results in a large amount of sludge, which is not easy to handle

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method of the composite biological agent for repairing threonine fermentation waste liquid comprises the following steps:

[0023] 1) Weigh the following raw materials in parts by weight for later use, including 20 parts of water, 8 parts of polyethylene glycol, 7 parts of humic acid, 5 parts of sucrose, 3 parts of kaolin, 2 parts of ammonium sulfate, 2 parts of chitin, silica gel 1 part powder;

[0024] 2) Crush humic acid with a crusher, then add kaolin, mix well, and grind to 100-mesh powder;

[0025] 3) Add water, polyethylene glycol, sucrose, ammonium sulfate, chitin and silica gel powder to the stirred reactor in sequence, stir at 200 rpm for 10 min, then add the powder in step 2), and stir at 500 rpm for 3 min, Then let it stand for 12 hours, and finally dry it at 60°C until the moisture content is lower than 5% (parts by weight), to obtain the biological carrier;

[0026] 4) Cultivate Bacillus subtilis, Lactobacillus acidophilus, Acinetobacter ...

Embodiment 2

[0029] The preparation method of the composite biological agent for repairing threonine fermentation waste liquid comprises the following steps:

[0030] 1) Weigh the following raw materials in parts by weight for later use, including 25 parts of water, 10 parts of polyethylene glycol, 8 parts of humic acid, 7 parts of sucrose, 4 parts of kaolin, 3 parts of ammonium sulfate, 3 parts of chitin, silica gel 2 parts of powder; wherein, the particle size of silica gel powder is 200 mesh;

[0031] 2) Crush humic acid with a crusher, then add kaolin, mix well, and grind to 100-mesh powder;

[0032] 3) Add water, polyethylene glycol, sucrose, ammonium sulfate, chitin and silica gel powder to the stirred reactor in sequence, stir at 200 rpm for 10 min, then add the powder in step 2), and stir at 500 rpm for 3 min, Then let it stand for 12 hours, and finally dry it at 60°C until the moisture content is lower than 5% (parts by weight), to obtain the biological carrier;

[0033] 4) Baci...

Embodiment 3

[0036] The effect test of biological preparation of the present invention:

[0037] The experiment was carried out by taking the fermentation waste liquid which removed fermentation cells, polysaccharides and polysaccharides as the treated sample.

[0038] Control group 1: use diatomaceous earth carrier; the type of microorganisms is the same as the bacteria and algae used in the test group; treatment method: after three days of continuous dosing, 5g / m3 per day, and then stand for six days, the detection and treatment results are shown in Table 1.

[0039]Control group 2: The carrier is the same as in Example 2, the difference is that only four kinds of bacteria are added, but Scenedesmus obliques is not added; treatment method: add 5g / cubic meter on the first day, and then leave it for six days to detect See Table 1 for the processing results.

[0040] Test group: the biological preparation prepared in Example 2 of the present invention; treatment method: one day after addin...

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Abstract

The invention relates to the field of the treatment of amino acid fermentation waste liquid, and discloses a preparation method of a composite biological agent for restoring threonine fermentation waste liquid. The preparation method comprises the steps of preparing a biological carrier, preparing composite microorganism liquid and preparing the composite biological agent. The preparation method is unique, the prepared biological agent is little in consumption and low in cost, and the capability for treating the pollutants is greatly improved.

Description

technical field [0001] The invention relates to the field of treatment of amino acid fermentation waste liquid, in particular to a preparation method of a composite biological preparation for repairing threonine fermentation waste liquid. Background technique [0002] Threonine (Threonine, abbreviated as Thr), scientific name 2-amino-3-hydroxybutyric acid, belongs to aliphatic amino acid, slightly sweet, named for its structure similar to threose, is a kind of human, animal and plant protein Essential amino acids are mainly used in medicine, chemical reagents, and nutritional enhancers. They can strengthen dairy products, restore human fatigue, and promote growth and development. In recent years, with the development of the economy, the market demand for threonine has continued to grow steadily, and it is one of the amino acid varieties with the fastest growing demand. Especially, the use in chemistry and biochemistry, food additives, feed additives, etc. has grown ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/23C12R1/01C02F103/36
CPCC12N1/20C02F3/34C02F2103/36C12N1/12
Inventor 张修军王均成张传森丁兆堂时夫龙卢松梁晓娟
Owner 内蒙古阜丰生物科技有限公司
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