Preparation method of composite biological agent for restoring threonine fermentation waste liquid
A technology for fermentation waste liquid and biological preparations is applied in the field of preparation of composite biological preparations, which can solve the problems of many times of feeding biological preparations, unreasonable microbial compatibility, and difficulty in light transmission, so as to achieve the effect of promoting nitrification and denitrification and reduce sludge. Yield, ease of transportation and preservation
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Embodiment 1
[0022] The preparation method of the composite biological agent for repairing threonine fermentation waste liquid comprises the following steps:
[0023] 1) Weigh the following raw materials in parts by weight for later use, including 20 parts of water, 8 parts of polyethylene glycol, 7 parts of humic acid, 5 parts of sucrose, 3 parts of kaolin, 2 parts of ammonium sulfate, 2 parts of chitin, silica gel 1 part powder;
[0024] 2) Crush humic acid with a crusher, then add kaolin, mix well, and grind to 100-mesh powder;
[0025] 3) Add water, polyethylene glycol, sucrose, ammonium sulfate, chitin and silica gel powder to the stirred reactor in sequence, stir at 200 rpm for 10 min, then add the powder in step 2), and stir at 500 rpm for 3 min, Then let it stand for 12 hours, and finally dry it at 60°C until the moisture content is lower than 5% (parts by weight), to obtain the biological carrier;
[0026] 4) Cultivate Bacillus subtilis, Lactobacillus acidophilus, Acinetobacter ...
Embodiment 2
[0029] The preparation method of the composite biological agent for repairing threonine fermentation waste liquid comprises the following steps:
[0030] 1) Weigh the following raw materials in parts by weight for later use, including 25 parts of water, 10 parts of polyethylene glycol, 8 parts of humic acid, 7 parts of sucrose, 4 parts of kaolin, 3 parts of ammonium sulfate, 3 parts of chitin, silica gel 2 parts of powder; wherein, the particle size of silica gel powder is 200 mesh;
[0031] 2) Crush humic acid with a crusher, then add kaolin, mix well, and grind to 100-mesh powder;
[0032] 3) Add water, polyethylene glycol, sucrose, ammonium sulfate, chitin and silica gel powder to the stirred reactor in sequence, stir at 200 rpm for 10 min, then add the powder in step 2), and stir at 500 rpm for 3 min, Then let it stand for 12 hours, and finally dry it at 60°C until the moisture content is lower than 5% (parts by weight), to obtain the biological carrier;
[0033] 4) Baci...
Embodiment 3
[0036] The effect test of biological preparation of the present invention:
[0037] The experiment was carried out by taking the fermentation waste liquid which removed fermentation cells, polysaccharides and polysaccharides as the treated sample.
[0038] Control group 1: use diatomaceous earth carrier; the type of microorganisms is the same as the bacteria and algae used in the test group; treatment method: after three days of continuous dosing, 5g / m3 per day, and then stand for six days, the detection and treatment results are shown in Table 1.
[0039]Control group 2: The carrier is the same as in Example 2, the difference is that only four kinds of bacteria are added, but Scenedesmus obliques is not added; treatment method: add 5g / cubic meter on the first day, and then leave it for six days to detect See Table 1 for the processing results.
[0040] Test group: the biological preparation prepared in Example 2 of the present invention; treatment method: one day after addin...
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