A kind of soil phosphorus microbial activator and application thereof
A microbial activator, soil phosphorus technology, applied in the direction of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of large increase in phosphorus quantity, unsatisfactory effect, and reduced production efficiency of phosphorus fertilizer, so as to promote absorption and utilization, alleviating demand contradictions, and good phosphorus-dissolving effect
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Embodiment 1
[0035] Example 1 Screening of preparation parameters of phosphorous activated microbial preparation
[0036] 1.1 Test materials
[0037] 1.1.1 Test strains and vectors
[0038] Test strain: Black fungus strain Mucor with the ability to solubilize phosphorus. The above black fungus strain Mucor has been deposited in the General Microbiology Center of the China Microbial Culture Collection Management Committee on September 4, 2014. The depository address: Beichen, Chaoyang District, Beijing No. 1 West Road No. 3 Institute of Microbiology, Chinese Academy of Sciences, its classification named Mucor sp., its deposit number is CGMCC No. 9637, the microscopic observation of this strain is as figure 1 Shown
[0039] Strain carrier: rice bran, activated carbon, sawdust
[0040] 1.1.2 Main test equipment
[0041] Constant temperature incubator, DHG-9053A drying oven, QHZ-98A shaker, 722 visible spectrophotometer, SW-CJ-IB aseptic operation table, grinder, high-pressure steam sterilizer, FA1104 e...
Embodiment 2
[0075] Example 2 Phosphate solubilization effect of three kinds of phosphorus microbial activators
[0076] See Example 1 for the preparation of required reagents
[0077] 2.1 Test of the effect of bacterial agent on dissolving phosphorus
[0078] Accurately weigh 1g of the inoculum into an Erlenmeyer flask, add 150mL of inorganic phosphorus culture solution, incubate at 140r / min, 26℃ for 72h in a shaker, and then determine the effective phosphorus concentration by molybdenum antimony colorimetric method (see 1.2.3 for specific operation methods) ), 3 groups of parallel experiments are set up at the same time, the experimental results are shown in Figure 7 .
[0079] by Figure 7 The analysis shows that the overall trend of the effective phosphorus content of the three carriers can be divided into two stages, firstly decreasing, and after reaching a valley point, increasing at a faster rate. This is also in line with the growth law of microorganisms. When there is phosphorus that ...
Embodiment 3
[0080] Example 3 The application of the microbial activator to soil
[0081] The phosphorus-activated microbial preparations screened in Example 1 were applied as base fertilizer to the growth area of the wheat demonstration base in Quanjiao County, Anhui Province. They were divided into test groups 1, 2, and 3 by area. The above-mentioned microbial activators were not used in the corresponding areas Set as the control group 1, 2, and 3 respectively. The test group and the control group were managed with the same management level during the wheat growth process. After ear setting, the number of effective ears per acre, number of spikelets per ear, number of bearing spikelets, and degenerated spikelets were counted. The results are shown in Table 2:
[0082] Table 2 Statistics of pre-production of wheat in phosphorus nutrient activator test
[0083]
[0084] According to the statistical results in Table 2, the effective ears per mu in Experiment 1 were 13,000 ears more than the con...
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