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A combined mycoplasma detection reagent and preparation method thereof

A technology for detecting reagents and mycoplasma, which is applied in the field of medicine, can solve the problems of unfavorable disease diagnosis and treatment, long time for interpreting results, etc., and achieve the effects of low pollution, high detection rate and strong selectivity

Inactive Publication Date: 2018-05-18
陕西瑞凯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it takes a long time to interpret the results of the solid culture method, so it takes a long time to diagnose the urogenital tract infection caused by UU and MH, which is not conducive to the diagnosis and treatment of the disease

Method used

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  • A combined mycoplasma detection reagent and preparation method thereof
  • A combined mycoplasma detection reagent and preparation method thereof
  • A combined mycoplasma detection reagent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A combined mycoplasma detection reagent consists of the following components:

[0020]

[0021] Wherein, the antibacterial agent is 18% peppermint water decoction, which can effectively inhibit the fungi of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Candida albicans, and other miscellaneous bacteria The growth of; the concentration of described dichlorophenolsulfonphthalein solution is 0.04%, and dichlorophenolsulfonphthalein solution is indicator, when dichlorophenolsulfonphthalein turns red from yellow in the culture medium, it can be proved that there are mycoplasma urealyticum and Mycoplasma hominis exists.

[0022] A preparation method of combined mycoplasma detection reagent, comprising the following steps:

[0023] 1) Dissolve the agar powder in 200ml of distilled water, autoclave to obtain solution I, keep the temperature of solution I at 45°C, and set aside;

[0024] 2) Add sericin, yeast peptone, ampicillin sodium s...

Embodiment 2

[0028] A combined mycoplasma detection reagent consists of the following components:

[0029]

[0030]

[0031] Wherein, the antibacterial agent is 18% peppermint water decoction, which can effectively inhibit the fungi of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Candida albicans, and other miscellaneous bacteria The growth of; the concentration of described dichlorophenolsulfonphthalein solution is 0.04%, and dichlorophenolsulfonphthalein solution is indicator, when dichlorophenolsulfonphthalein turns red from yellow in the culture medium, it can be proved that there are mycoplasma urealyticum and Mycoplasma hominis exists.

[0032] A preparation method of combined mycoplasma detection reagent, comprising the following steps:

[0033] 1) Dissolve the agar powder in 200ml distilled water, and sterilize under high pressure to obtain solution I, keep the temperature of solution I at 47.5°C, and set aside;

[0034] 2) Add sericin,...

Embodiment 3

[0038] A combined mycoplasma detection reagent consists of the following components:

[0039]

[0040]

[0041] Wherein, the antibacterial agent is 18% peppermint water decoction, which can effectively inhibit the fungi of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Candida albicans, and other miscellaneous bacteria The growth of; the concentration of described dichlorophenolsulfonphthalein solution is 0.04%, and dichlorophenolsulfonphthalein solution is indicator, when dichlorophenolsulfonphthalein turns red from yellow in the culture medium, it can be proved that there are mycoplasma urealyticum and Mycoplasma hominis exists.

[0042] A preparation method of combined mycoplasma detection reagent, comprising the following steps:

[0043] 1) Dissolve the agar powder in 200ml of distilled water, autoclave to obtain solution I, keep the temperature of solution I at 50°C, and set aside;

[0044] 2) Add sericin, yeast peptone, ampicil...

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Abstract

The invention discloses a combined type mycoplasma detection reagent. The combined type mycoplasma detection reagent comprises 6.5-10g of yeast peptone, 0.015-0.025g of ampicillin sodium, 0.5-1.0g of L-arginine, 0.001-0.003g of thallous acetate, 4-12g of agar powder, 0.02-0.1g of urea, 2.25-2.75g of lactalbumin hydrolysate, 0.01-0.05g of sericin, 0.017-0.023g of chlorophenol red liquor, 0.003-0.006g of genipin acid, 0.001-0.01g of toad dioscin and 10-15ml of bacteriostat. The combined type mycoplasma detection reagent has the advantages that mycoplasma urealyticum and mycoplasma hominis can be simultaneously and quickly detected by the detection reagent, a detection method is simple, and the combined type mycoplasma detection reagent is short in time and high in selectivity, sensitivity and detection rate.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a combined mycoplasma detection reagent and a preparation method thereof. Background technique [0002] Mycoplasma is a common pathogen of sexually transmitted urogenital diseases. The detection rate accounts for 40-70% of the STD population. Mycoplasma infection can lead to diseases such as genitourinary tract inflammation, prostatitis, pelvic inflammatory disease, male infertility, female infertility, villous gland inflammation and low birth weight infants. Ureaplasma urealyticum and Mycoplasma hominis are mainly related to urogenital tract infection. About 20-30% of patients with non-gonococcal urethritis are caused by Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH). It is the second most common pathogen of non-gonococcal urethritis and cervicitis. Therefore, the detection of Ureaplasma urealyticum and Mycoplasma hominis is clinically important. [0003] At present...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12R1/35
Inventor 高琪张鲁阳
Owner 陕西瑞凯生物科技有限公司
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