Fermented soybean meal production process suitable for large-scale production

A technology for fermented soybean meal and production process, applied in the field of fermented soybean meal production process, can solve the problems of large-scale solid-state fermentation process stability, poor product stability, uneven material distribution, etc., to avoid bacterial growth restriction and improve production performance , Increase the effect of mixing uniformity

Inactive Publication Date: 2015-12-09
山东祥维斯生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0015] In order to avoid the problem of the stability of the large-scale solid-state fermentation process in the prior art, the present invention provides a stable fermented soybean meal production process suitable for large-scale production. The method of the present invention can overcome the existing difficulties in operation and uneven material distribution. The defects of uniformity and poor product stability

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  • Fermented soybean meal production process suitable for large-scale production

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Effect test

Embodiment 1

[0085] Step 1. Preparation of seed liquid in seed medium: tryptone 1.5g, glucose 3.0g, yeast extract 1.5g, sodium chloride 0.5g, water 100mL, medium filling volume 100mL / 500mL Erlenmeyer flask, 121°C After 20min of sterilization, the concentration was 10 8 The rhizopus aureus of each / mL was inserted into the seed medium according to the inoculum amount of 5%, and cultivated at 30°C for 20 hours, and the thalline concentration reached 25;

[0086] Step 2, preparation of fermentation medium: 70% of soybean meal, 24% of bran, 5% of sucrose, 1% of betaine hydrochloride, adding water to make the material-water ratio reach 0.8, mixing evenly; after that, high-temperature sterilization at 121°C for 1 hour, and natural cooling;

[0087] Step 3. Fermentation culture: In step 1, the seed solution was inserted into the fermentation medium with an inoculation amount of 5%, and fermented at 30°C; after 16 hours of fermentation, spray and add 0.5% microfiltration sterilized Betaine hydroch...

Embodiment 2

[0092] The difference between this embodiment and embodiment 1 is that step 1 is omitted, and 10 8 Rhizopus aureus per mL was inserted into the fermentation medium according to the inoculum size of 5%.

[0093] In this example, the use of a single strain of Rhizopus aureus to ferment soybean meal greatly reduces the probability of fermentation contamination, and can effectively eliminate trypsin inhibitors, phytic acid, soybean hemagglutinin, urease, oligosaccharides, and lipoxygenase in soybean meal , soybean antigenic protein (allergenic factor) and goiter and other anti-nutritional factors and achieve a good protein degradation effect, the content of small molecular proteins and small peptides accounts for more than 70% of the total protein, and promote small peptides and their binding minerals The absorption of elements promotes the growth of animals.

Embodiment 3

[0095] The difference between this embodiment and embodiment 1 is that the amount of betaine hydrochloride added by spraying in step 3 is 1%, and the others are the same as in embodiment 1.

[0096] In this example, compared with Example 1, the amount of betaine was increased, based on the following reasons: adding betaine with sweet taste in soybean meal fermentation can reduce the surface interfacial tension of the material and increase the uniformity of material mixing; In addition, betaine can regulate the permeability of the cell membrane of the bacteria, avoiding the growth restriction of the bacteria caused by dissolved oxygen;

[0097] Moreover, although the fermented soybean meal with a single strain of Rhizopus pachyrhiza has greatly reduced the probability of fermentation contamination, and can effectively eliminate anti-nutritional factors in the soybean meal and achieve a good protein degradation effect, its palatability and food-attracting effect are slightly poor...

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Abstract

The invention discloses a fermented soybean meal production process suitable for large-scale production and belongs to the technical field of soybean meal processing. The process comprises the steps of mixing tryptone, glucose, yeast extract and sodium chloride, sterilizing at high temperature, adding less-root rhizopus oryzae into a seed culture medium for culturing and preparing a seed culture medium; mixing bean meal, bran, sucrose and glycine betaine, adding water and uniformly mixing; sterilizing at high temperature and naturally cooling to prepare a fermented culture medium; inoculating the seed culture medium into the fermented culture medium and fermenting for culture; adding a glycine betaine solution in a spraying manner after fermenting, and stopping fermenting after spraying; drying and grinding; drying a fermented product, grinding and sieving to obtain a fermented bean meal finished product. According to the process, seed enlarged cultivation is performed, and stability of a production technology is realized; the glycine betaine is added in fermentation to increase the mixing uniformity of the materials; single less-root rhizopus oryzae strains are adopted to ferment the bean meal, so that the fermentation contamination probability is greatly reduced; the process is mature and stable and suitable for large-scale production.

Description

technical field [0001] The invention relates to the technical field of soybean meal processing, in particular to a fermented soybean meal production process that enhances the degradation effect of anti-nutritional factors, increases palatability, and is suitable for large-scale production. Background technique [0002] Soybean meal is a by-product obtained after extracting soybean oil from soybeans. Soybean meal is generally in the form of irregular fragments, the color is light yellow to light brown, and the taste has the aroma of roasted soybeans. The main components of soybean meal include: protein 40% to 48%, lysine 2.5% to 3.0%, tryptophan 0.6% to 0.7% and methionine 0.5% to 0.7%. Soybean meal is one of the 12 kinds of animal and vegetable oil meal feed products, including cottonseed meal, peanut meal, and rapeseed meal, which has the largest output and is the most widely used; as a kind of high protein, soybean meal is the main raw material for making livestock and pou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/14
Inventor 马兴群吕丽娟宋琦刘雨
Owner 山东祥维斯生物科技股份有限公司
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