Method for rapid detection of whole blood erythrocyte eicosapentaenoic acid
A technology of eicosapentaenoic acid and red blood cells, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of the inability to perform specific qualitative and quantitative analysis of various fatty acids, and the inability to achieve accurate qualitative separation and quantitative analysis, and analyze the results Problems such as poor reproducibility, to achieve the effect of improving detection accuracy, powerful quantitative function, and good accuracy
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Embodiment 1
[0028] The pretreatment of embodiment one whole blood sample
[0029] Mix the anticoagulated whole blood sample, centrifuge, discard the upper layer of plasma, white blood cells, platelets, etc.; add an equal volume of normal saline to the remaining red blood cells, mix and centrifuge, discard the upper layer of normal saline; then add the remaining red blood cells Add an equal volume of normal saline and mix well to obtain the separated red blood cell sample.
[0030] Take 50 μL red blood cell sample, put it in a glass centrifuge tube with screw cap, add heptadecanoic acid as an internal standard solution, add 1 mL of 3mol / L hydrochloric acid-methanol solution, mix well, put it in an oven, react at 90°C for 3 hours in the dark, and derivatize After the reaction was completed, cool to room temperature, add 2 mL of n-hexane to extract the methyl esterification reaction product, take the supernatant and dry it with nitrogen, then add n-hexane to the residue as a reconstitution...
Embodiment 2
[0032] Example 2 Detection of whole blood erythrocyte fatty acids
[0033] The sample detection solution is detected by gas chromatography-mass spectrometer.
[0034] The chromatographic conditions include: the chromatographic column is a polar capillary column with a specification of 20m*0.18mm (ID)*0.20μm (film) (purchased from Agilent, model DB-23, PartNumber122-2361), and the carrier gas is 99.999% pure Helium, the carrier gas flow rate is 0.90mL / min, the sampling method is splitless injection, the injection volume is 1 μL, the inlet temperature is 230°C, the post-run temperature is 240°C, the post-run time is 2min, and the purge The flow rate is 4.0mL / min, and the total flow rate is 55mL / min. The temperature programming conditions are shown in Table 1.
[0035]
[0036] Mass spectrometry detection adopts electron bombardment ionization mode, and adopts full scan and selected ion scan methods. Mass spectrometry conditions include: ionization source is electron bomb...
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