Green pig feed additive
A feed additive and green feed technology, applied in animal feed, animal feed, application, etc., can solve the problems of low pig absorption rate, poor compatibility, and environmental pollution, and achieve good compatibility, low environmental pollution, and high absorption rate Effect
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Embodiment 1
[0032] Example 1: Acquisition of marine Rhodotorula WYN1 and domestication of copper tolerance
[0033] (1) Fresh prawns, starfish, seaweed and other biological samples will be collected from the Yellow Sea and placed in a sterile YEPD medium, and red or pink round colonies will be picked for further purification and culture. According to the accumulation of carotenoid of each type of bacteria, the bacteria with high carotenoid production were selected. Among them, the marine red yeast WYN1, which produces higher carotenoids, has oval cells without pseudohyphae; red colonies are formed on YEPD medium with seawater, with smooth surfaces and neat edges; no seeds are produced on sporulation medium Cystospores, multilateral budding, no spore-bore; no sugar fermentation, no starch-like compounds, different inositol. Based on the above characteristics, it is preliminarily determined to belong to the genus Rhodotorula. Keep the slope in the refrigerator.
[0034] (2) Fermentation cultu...
Embodiment 2
[0050] Example 2: (Comparative test) Research on fermentation conditions of marine red yeast WYN1 with high-yield carotenoids
[0051] Fermentation medium composition: glucose 2.5%, peptone 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, copper content 300mg / L (copper sulfate configuration), sodium chloride 20%, the rest is water; the initial pH is 8 , The inoculation amount is 10%, the rotating speed of the shaker is 180r / min, after 24 hours of incubation at 28°C, the temperature is adjusted to 30°C, the pH is adjusted to 5, and the nitrogen source is added once, that is, peptone is added at the amount of 2.5%, continue Fermentation for 24h. Using the above method to ferment and culture marine red yeast WYN1, its biomass, carotenoid production and intracellular copper content were 27.52g / L, 25.12mg / L and 6240μg / g dry bacteria, respectively.
Embodiment 3
[0052] Example 3: Feed additives and their preparation
[0053] The weight parts of feed additives are as follows:
[0054] Epimedium extract 10, marine red yeast fermentation culture 15, wolfberry enzymatic hydrolysis powder 6, licorice 5, tamarind 5, houttuynia 2, dandelion 3; the marine red yeast preservation number is CCTCCNO: M2014592.
[0055] The preparation method of the epimedium extract is as follows:
[0056] After the epimedium is crushed through a 40-50 mesh sieve, add a 50% ethanol solution of 8 times the weight of the epimedium, and use 50kHz ultrasonic to extract twice at room temperature, 30min each time, filter to obtain the ethanol extract, the residue after extraction Add 8 times the weight of hot water, decoct at 80°C for 1 hour, mix the filtered solution with the ethanol extract, heat it to concentrate the extract, spray dry, and pulverize through a 60-mesh sieve to obtain the Epimedium extract.
[0057] The preparation method of the wolfberry enzymatic hydrolysis...
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