Formylphenylboronic acid modified magnetic nanoparticles and preparation and application thereof

A technology of magnetic nanoparticles and formyl phenylboronic acid, which is applied in the preparation of test samples, other chemical processes, chemical instruments and methods, etc., can solve the problems of cumbersome synthesis steps, achieve strong enrichment ability, good application prospects, The effect of simple method

Active Publication Date: 2016-02-10
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the reported boronic acid-modified hybrid magnetic nan

Method used

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  • Formylphenylboronic acid modified magnetic nanoparticles and preparation and application thereof
  • Formylphenylboronic acid modified magnetic nanoparticles and preparation and application thereof
  • Formylphenylboronic acid modified magnetic nanoparticles and preparation and application thereof

Examples

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Embodiment 1

[0019] Phenylboronic acid modified magnetic nanoparticles (Fe 3 o 4 PEI-FPBA) synthesis. The whole synthesis reaction is divided into two steps:

[0020] In the first step, Fe was first synthesized by a one-pot solvothermal reduction method 3 o 4 PEI nanoparticles: use 1.0g ferric chloride hexahydrate as raw material, use 50mL ethylene glycol as the reaction solvent, add 4.0g sodium acetate and 1.0g PEI (weight average molecular weight 70000Da), stir evenly and move to a 100mL reaction kettle, The reaction temperature is 220°C, the reaction time is 2h, Fe 3 o 4 PEI magnetic nanoparticles.

[0021] The second step of phenylboronic acid modification: After washing the material synthesized in the first step with water and ethanol alternately for 3 times, take 0.2 g of the material and disperse it in 300 mL of glacial acetic acid / methanol solution with a volume ratio of 1:124, and add 0.2 g of 4- Formylphenylboronic acid and 0.2g sodium cyanoborohydride react at 40°C for 10h ...

Embodiment 2

[0024] Selective enrichment of catecholamines in urine:

[0025] (1) Urine pretreatment: the urine (containing antioxidants) was thawed, centrifuged at 14,000 rpm / min for 15 min, and ammonia water was added to adjust the pH of the urine to 8.5.

[0026] (2) Enrichment of catecholamines: take 1 mL of urine adjusted to alkaline, add 8 mg of the phenylboronic acid-modified magnetic nanomaterial Fe in Example 1 3 o 4 For PEI-FPBA, ultrasonically disperse for 1 min, then vortex for 2 min. After the magnetic material is separated by a magnet, the supernatant is removed, the material is washed three times with a pH 8.5 ammonia solution (containing 50% acetonitrile), and then 1 mL of a pH 3.5 formic acid solution (containing 80% acetonitrile) is added for elution, ultrasonication for 1 min, and vortex 1min. The magnetic nanomaterials were removed by magnetic separation, and 800 μL of the eluent was taken to freeze-dry, and then reconstituted with 40 μL of reconstitution solution, a...

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Abstract

The invention belongs to the technical field of inorganic materials and analysis and particularly relates to a simple preparation method for formylphenylboronic acid modified magnetic nanoparticles (Fe3O4@PEI-FPBA) and application of the formylphenylboronic acid modified magnetic nanoparticles in selective enrichment of catecholamines in urine. Magnetic microspheres Fe3O4@PEI are synthesized by a one-pot method and then are grafted with phenylboronic acid functional groups through reductive amination reaction so as to form the hybridized magnetic nanoparticles. The magnetic material has the characteristics of simple preparation method, high adsorption capacity, good selectivity and short balancing time; and when the magnetic material is applied to the selective enrichment of a trace amount of catecholamines in the urine, the pretreatment process is simple and rapid and is high in specificity. The material has an excellent application prospect in the enrichment of a 1,2-cis-dihydroxy compound in body fluids.

Description

technical field [0001] The invention belongs to the field of inorganic materials and analysis technology, and specifically relates to a formylphenylboronic acid-modified magnetic nanoparticle (Fe 3 o 4 A simple preparation method of PEI-FPBA) and its application. [0002] The magnetic nanoparticles designed and proposed by the invention have a simple preparation process and can be used as an affinity probe to achieve highly selective separation and enrichment of trace 1,2-cis-dihydroxy compounds in complex biological samples. The material has good application prospects in the field of metabolomics. Background technique [0003] Catecholamines mainly include dopamine (DA), epinephrine (EP), norepinephrine (NE) and isoproterenol (IP). The chemical structures of these substances have a common feature: they all have a dihydroxybenzene nucleus and a side chain with an amino group. This type of substance is an endogenous active substance of organisms, has strong biological eff...

Claims

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Application Information

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IPC IPC(8): B01J20/286B01J20/30B01D15/38G01N1/40
Inventor 许国旺秦倩石先哲李华
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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