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Strain for producing griflola frondosa polysaccharides and application thereof

A technology for polysaccharides and strains of Grifola frondosa, applied in the direction of fungi, microorganism-based methods, microorganisms, etc., can solve the problems of low polysaccharide content, difficult to obtain strains, and low mycelium yield, and achieve simple ingredients and reduced production costs , the fermentation effect is remarkable

Inactive Publication Date: 2016-03-30
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, conventional grifola frondosa polysaccharide-producing strains have the problems of low polysaccharide content and low mycelium yield, especially grifola frondosa strains suitable for large-scale fermentation production are difficult to obtain

Method used

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  • Strain for producing griflola frondosa polysaccharides and application thereof
  • Strain for producing griflola frondosa polysaccharides and application thereof
  • Strain for producing griflola frondosa polysaccharides and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Screening and Identification of Example 1 Bacterial Strains

[0038] 1. Screening of enzyme-producing strains

[0039] The present invention collects more than ten strains of Grifola frondosa fungus from the chestnut producing area in Jingdong area to screen the production strains producing Grifola frondosa polysaccharides, and picks and cultivates vigorous, dense and white hyphae grown on PDA and potato semi-combined medium For the fermentation and production of polysaccharides from Grifola frondosa, the strains were identified and stored in PDA medium in cold storage. Specific steps are as follows:

[0040] Inoculate more than ten strains of Grifola frondosa fungus collected from the chestnut producing area in Jingdong area on PDA and potato semi-combined medium, culture at 25°C-30°C for 5-15 days, select vigorously growing mycelium, and obtain the fermented The strain with the highest polysaccharide content was selected as Griflolafrondosa and named Griflolafrondos...

Embodiment 2

[0062] The bacterial strain GrT.01 that embodiment 2 obtains by screening is carried out the experimental determination of the optimal condition of fermentation Grifola frondosa mycelium:

[0063] 1) The selection and determination experiment of the carbon source in the fermentation medium:

[0064] First, set up 10 experimental groups, in which, except carbon source in the fermentation medium, nitrogen source

[0065] For bran juice, pH 6, KH 2 PO 4 and MgSO 4 The mass percentage concentrations were 0.3% and 0.15%, the growth factor was 0.2% and the temperature was 25°C, and the individual carbon sources were respectively 2% glucose, sucrose, soluble starch, cornstarch, corn flour, Mashed potatoes, compound carbon source Add 2% concentration of soluble starch, corn flour, corn starch, and mashed potatoes on the basis of 2% glucose concentration.

[0066] After the above-mentioned ten experimental groups were fermented and cultured, the mycelium biomass of each experimenta...

Embodiment 3

[0072] The method for producing Grifola frondosa polysaccharide-producing strains described in Example 3 includes the following steps: preparing seed liquid: inoculating the screened bacterial strain GrT. After 5-8 days of fermentation, the logarithmic growth phase is reached, the fermentation is stopped, and the seed liquid is obtained;

[0073] Large-scale reproduction of strains: after the seed liquid is inoculated on the primary fermentation medium, the primary fermentation medium is continuously fermented for 5-8 days under the conditions of pH 4-8 and temperature 23-28°C, so that the The strains were mass-propagated to produce polysaccharides. After the fermentation, the biomass of Grifola frondosa was 37g / L (dry weight), and the polysaccharide content reached 17%.

[0074] Figure 5 It is a comparison chart of the biomass of the strains of the present invention in the fermentation medium induced by different inducers. Figure 6 It is a comparative determination chart...

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Abstract

The invention discloses a strain for producing griflola frondosa polysaccharides and an application thereof; the strain has the preservation number of CGMCC No.10116 in China General Microbiological Culture Collection Center and has the preservation date of December 9, 2014; the strain is classified and named as griflola frondosa GrT.01. The strain GrT.01 is obtained through separation and screening of a wild environment natural fruiting body, has high content of mycelia and polysaccharides, and can be applied for high-efficiency production of the griflola frondosa polysaccharides. The strain GrT.01 has the advantages of simple fermentation culture medium ingredients, low price and significant fermentation effects, is general and easy to obtain in industrial production, makes the production cost of the griflola frondosa polysaccharides greatly reduced, and can be applied to large-scale liquid submerged fermentation production.

Description

technical field [0001] The invention belongs to the field of food biotechnology, and in particular relates to a bacterial strain for producing grifola frondosa polysaccharide and application thereof. Background technique [0002] Grifola frondosa (Grifolafrondosa) is a large fungus in the genus Basidiomycotina, Laminomycetes, Non-septa-Basidiomycetes, Non-Pleatellales, Polyporacea, also known as Bayleaf Polypore, Chestnut Mushroom, Heavy Mushroom Wait, the Japanese call it maitake. [0003] In the past 20 years, research on fungal polysaccharides as biological response regulators has been carried out at home and abroad, and the remarkable curative effect of Grifola frondosa polysaccharides has gradually been recognized by people. After a lot of chemical and pharmacological analysis, it is found that the polysaccharide of Grifola frondosa has obvious anti-tumor, anti-HIV virus, improving immune system function, regulating blood sugar, blood lipid and cholesterol levels, lowe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/04C12R1/645
CPCC12P19/04C12N1/145C12R2001/645
Inventor 陈利梅赫荣琳陈树林
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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