Method for producing polymyxin E through fermentation and foam separation coupling

Abstract

The invention provides a method for producing polymyxin E through fermentation and foam separation coupling. According to the method, polymyxin E is prepared by a circulating reflux device formed by a peristaltic pump through fermentation, film separation and foam separation coupling. During coupling operation, fermentation liquor enters a foam separation tower through a film filter by means of the peristaltic pump, products and a liquid pass through the film while cells are intercepted in a fermentation tank, fermentation continues to be performed, and constancy of bacteria is guaranteed; a foam distributor is mounted at the bottom of the foam separation tower, bubbles are produced through air blowing, foam separation is performed, part of nutrients enter the foam separation tower through the film filter, a remaining liquid produced after foam separation returns to the fermentation tank in a circulating reflux manner, and sufficient utilization of the nutrients is guaranteed; coupling outside a tank body is adopted, operation conditions for fermentation and operation conditions for foam separation are controlled respectively, and the best operation conditions can be met.

Description

Technical field

[0001] The invention relates to the technical field of bioengineering, in particular to a system and method for producing polymyxin E by coupling fermentation and foam separation. Background technique

[0002] Polymyxin E has the characteristics of good antibacterial spectrum, high efficiency, low toxicity, and less residue. It can be used as feed additives to promote the growth of animals and improve feed utilization. It can also prevent the large intestine Escherichia coli that often occurs in feed in large-scale production. For the diseases caused by the contamination of Salmonella and Salmonella, my country has listed polymyxin E as a national legal species, but the product has a low potency and mainly depends on imports. This product is mainly produced by fermentation. The traditional separation method mainly uses ion exchange chromatography, which consumes a lot of energy. This research group uses the foam separation method to separate polymyxin E from the f...

Images