Composite microsphere marker for conducting fluorescent marking on rear earth and preparation method of composite microsphere marker

Abstract

The invention provides a composite microsphere marker for conducting fluorescent marking on rear earth and a preparation method of the composite microsphere marker and relates to the technical field of functional polymer biomaterials. The preparation method includes the steps that polyvinylpyrrolidone, potassium persulfate / persulphate and styrene are added to an ethyl alcohol medium or a water medium to be dissolved and then placed into an ultrasonic-wave reactor, a polymerization reaction is generated under protection of nitrogen gas, and polystyrene nanometer microsphere dispersion liquid is obtained; then rare earth fluorescence complex 4, 7-dichloro sulfo benzene-1, 10 phenanthroline-2, 9 dicarboxylic acid-europium or 4, 4-di (1, 1, 1, 2, 2, 3, 3, -heptafluoride-4,6,-adipic ketone group) chlorine sulfo-adjacent-terphenyl- europium is fixed to the surface of a silicon dioxide microsphere in a chemical bonding mode, and finally the composite microsphere marker for conducting fluorescent marking on rear earth is prepared. The composite microsphere marker has the advantages of being long in fluorescent lifetime, good in stability, easy to synthesize, low in cost and easy to mark.

Description

technical field [0001] The invention relates to the technical field of functional polymer biomaterials, in particular to a rare earth fluorescent-labeled composite microsphere marker and a preparation method thereof. As a fluorescent material with excellent performance, rare earth fluorescent microsphere markers can be widely used in the medical field. Background technique [0002] In the prior art, there have been many reports on rare earth ions or rare earth complexes and silicon dioxide-doped luminescent materials, such as Cannasa (Cannasa.C, etal, Mat.Res.Soc.Symp.Proc.676(Y3)18.1 -18.6, 2001) described the preparation of directly doped europium (Eu 3+ ) Y2O3-SiO2 nanoluminescent material; Moran et al. (C.E.Moranetal, Langmuir2001, 17, 8376-8379) synthesized silicon nanoparticles doped with Pr3+ and Er3+; Cheng Yanling et al. , 1217-1220) with polyvinylpyrrolidone as a stabilizer, the monomer styrene was dispersed and polymerized by ultrasonic irradiation to prepare po...

AI Technical Summary

Problems solved by technology

[0003] Among the existing rare earth fluorescent complex markers, commonly used fluorescent markers, such as fluorescein isothiocyanate (FITC), rhodamine-6-G (R-6-G), etc., have relatively short fluorescence lifetimes. , long-term use is difficult to overcome the interference of background fluorescence, and the sensitivity is usually low in its detection; the rare earth ions are mainly Eu 3+ 、Sm 3+ 、Dy 3+ and Tb 3+ The complexes formed with some organic complexes such as β-diketone compounds, o-phenanthroline compounds, salicylic acid compounds, bipyridine compounds, etc., have been used to varying degrees, but it is difficult to meet the requirements simultaneously. The following requirements: (1), strong fluorescence; (2), good stability: including thermodynamic stability and resistance to photobleaching, etc.; (3), easy to cross-link with biological macromolecules such as antibodies and nucleic acids, and can be maintained at the same time after cross-linking The high fluorescence intensity of the marker and the biological activity of the marker; (4) easy to synthesize

Images