Preparation method of Brevibacillus atrophaeus wettable powder

Abstract

The invention relates to the fields of biopesticide, and specifically discloses a Brevibacillus atrophaeus wettable powder containing a spore germinator, and a preparation method thereof. The wettable powder consists of the following components by weight: 40-70 parts of a Brevibacillus atrophaeus fermentation broth, 20-30 parts of a kaolin filler, 1-4 parts of a wetting agent sodium lauryl sulfate, 0.2-1 part of a dispersant of Morwet D-425, a spore germinator containing 0.1-1 part of fructose, 0.1-1 part of threonine and 0.1-0.5 part of pyridine-2,6-dicarboxylic acid calcium, a UV protective agent containing 1-2 parts of mycose and 0.2-1 part of carboxymethyl cellulose, and a drying protecting agent containing 1-5 parts of cane sugar and 0.2-1 part of monosodium glutamate. After application of the Brevibacillus atrophaeus wettable powder into the field, the spores have high germination speed and high germination rate; and the control effect of the Brevibacillus brevis wettable powder can be significantly improved, the usage amount of spores and the production costs can be reduced.

Description

technical field [0001] The invention relates to the technical field of microbial pesticides, in particular to a Bacillus atrophaeus wettable powder containing a spore germination agent and a preparation method thereof. Background technique [0002] Bacillus atrophaeus (Bacillus atrophaeus, non-pathogenic bacteria, a type of variant of Bacillus subtilis, can form soluble black colonies on medium containing carbohydrates, mainly isolated from soil. Used in agriculture, industry, scientific research, It is widely used in medical and sanitation fields. Bacillus atrophaeus can produce catalase, but not contact enzyme, VP reaction is positive, does not produce hydrogen sulfide, indole and dihydroxyacetone, and can reduce nitrate to nitrite Salt can hydrolyze starch and casein. The optimum growth temperature range of Bacillus atrophaeus: 28~30℃; the maximum growth temperature range: 50~55℃; the minimum growth temperature range: 5~10℃. 4-9 and grow in 7% NaCl. Bacillus atrophaeus c...

AI Technical Summary

Problems solved by technology

[0010] At the present stage, there is no technical scheme disclosed in the Bacillus atrophaeus wettable powder, and there is no report on the research of adding a spore germination agent in the Bacillus atrophaeus wettable powder

[0011] In addition, due to the compatibility of different bacillus with wetting agents, dispersants and other additives, the carrier has different adsorption properties to various bacillus, and the existing formula or preparation process of bacillus wettable powder cannot be used Prepare the wettable powder of Bacillus atrophaeus added with spore germination agent, the adsorption amount of effective bacteria in the wettable powder, the survival rate, the control effect on harmful bacteria and the stability of the wettable powder dose are quite different. The preparation method must fully consider the adsorption capacity of each carrier, the protective effect on the drying process of Bacillus atrophaeus, the influence of wetting agents and dispersants on the suspension rate of wettable powders, and the wetting time, and a comprehensive screening of spore germination agents can be achieved. Get a wettable powder with short wetting time, high suspension rate, long storage time of spores, rapid germination of spores after use, and good control effect on Bacillus atrophaeus