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Preparing method and application of electrochemiluminescence Clenbuterol sensor based on magnetic two-dimensional nanocomposite

A two-dimensional nano-composite material technology, applied in the field of electrochemiluminescence clenbuterol sensor preparation, can solve the problems of low sensitivity of electrochemiluminescence sensor, unfavorable practical application, poor conductivity, etc. Effect of chemiluminescence intensity, enhancement of resonance energy transfer capability, enhancement of intensity and stability

Inactive Publication Date: 2016-07-06
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the poor conductivity of titanium dioxide also limits the sensitivity of electrochemiluminescence sensors constructed from a single titanium dioxide nanomaterial, which is not conducive to practical applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 FeMn-TiO 2 / g -C 3 N 4 preparation of

[0037] First, take 0.8mmol of iron salt and 0.8mmol of manganese salt and add them to 5mL of tetrabutyl titanate. During stirring, slowly add 0.5mL of hydrofluoric acid, and react in the reactor at 160°C for 24 hours. After cooling to room temperature, After centrifugal washing with ultrapure water and absolute ethanol three times, vacuum-dry at 50°C; secondly, mix 150 mg of the dried solid with 400 mg of melamine, and grind it into powder; then, put the ground powder into a muffle furnace, The heating rate is 1°C / min, and calcined at 480°C for 5 hours; finally, the calcined powder is cooled to room temperature to obtain FeMn-TiO 2 / g -C 3 N 4 ;

[0038] Described iron salt is ferric sulfate;

[0039] Described manganese salt is manganese sulfate.

Embodiment 2

[0040] Example 2FeMn-TiO 2 / g -C 3 N 4 preparation of

[0041] First, take 0.8mmol of iron salt and 1.0mmol of manganese salt and add them to 5mL of tetrabutyl titanate. During stirring, slowly add 0.65mL of hydrofluoric acid, and react in the reactor at 180°C for 21 hours. After cooling to room temperature, After centrifuging and washing three times with ultrapure water and absolute ethanol, vacuum-dry at 50°C; secondly, mix 200 mg of the dried solid with 400 mg of melamine, and grind it into powder; then, put the ground powder into a muffle furnace, The heating rate is 2°C / min, and calcined at 520°C for 2 hours; finally, the calcined powder is cooled to room temperature to obtain FeMn-TiO 2 / g -C 3 N 4 ;

[0042] Described iron salt is ferric chloride;

[0043] Described manganese salt is manganese chloride.

Embodiment 3

[0044] Example 3 FeMn-TiO 2 / g -C 3 N 4 preparation of

[0045] First, take 0.8mmol of iron salt and 1.2mmol of manganese salt and add them to 5mL of tetrabutyl titanate. During stirring, slowly add 0.8mL of hydrofluoric acid, and react in the reactor at 200°C for 18 hours. After cooling to room temperature, After centrifuging and washing with ultrapure water and absolute ethanol three times, vacuum-dry at 50°C; secondly, mix 250 mg of the dried solid with 400 mg of melamine, and grind it into a powder; then, put the ground powder into a muffle furnace, The heating rate is 3°C / min, and calcined at 560°C for 0.5 hours; finally, the calcined powder is cooled to room temperature to obtain FeMn-TiO 2 / g -C 3 N 4 ;

[0046] Described iron salt is ferric nitrate;

[0047] Described manganese salt is manganese nitrate.

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Abstract

The invention discloses a preparing method of an electrochemiluminescence Clenbuterol sensor based on a magnetic two-dimensional nanocomposite, and belongs to the technical field of novel nanometer functional materials and biosensors. The preparing method comprises the steps that firstly, a novel bi-metal co-doped two-dimensional manometer material FeMn-TiO2 / g-C3N4 is prepared, and a Clenbuterol antibody is loaded by using the good biocompatibility and the large specific area of the material; secondly, horse radish peroxidase is fixed through the cross-linking action of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide to generate oxygen in situ and is subjected to an electrochemical reaction with a base solution to generate high and stable electrochemiluminescence signals, then the influence on the electron transmittability by the specific and quantitative combination of the antibody and an antigen is used, the electrochemiluminescence signals are reduced correspondingly, and finally the Clenbuterol detecting electrochemiluminescence biosensor low in cost, high in sensitivity and specificity, fast in detection and easy to prepare is obtained.

Description

technical field [0001] The invention relates to a preparation method of an electrochemiluminescence clenbuterol sensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Clenbuterol, that is, clenbuterol hydrochloride, can significantly improve the lean meat percentage, weight gain and improve feed conversion ratio. Therefore, it is also called lean meat powder in China, and it has been used as a growth promoter and feed additive for cattle, sheep, poultry, pigs and other livestock and poultry. However, because clenbuterol is too harmful to human health and poses safety hazards, it has been banned globally. [0003] At present, the methods for detecting clenbuterol mainly include chromatography and mass spectrometry. Such methods require expensive instruments and complex operations, and laboratory personnel need professional training before they can perform detection. Therefore, it is of great significa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 张勇杜斌马洪敏吴丹范大伟
Owner UNIV OF JINAN
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