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A magnetic resonance imaging contrast agent based on nano-graphene oxide and its preparation method

A nano-graphite oxide and magnetic resonance imaging technology, applied in the field of radiodiagnosis, can solve the problems of poor dispersion of physiological solutions, unfavorable promotion and transformation, complicated preparation process, etc. good biocompatibility

Inactive Publication Date: 2019-01-11
FUDAN UNIV SHANGHAI CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current gadolinium ion-based MRI contrast agents using graphene oxide as a carrier still have deficiencies and defects, including: 1) due to the lack of proper functional modification of the graphene oxide used, the dispersion in physiological solutions is poor, and biological Low compatibility, it is difficult to apply in vivo injection, and lacks the possibility of transformation application; 2) Although a certain functional modification is carried out on the graphene oxide used, it still needs to introduce an additional chelating agent, resulting in a complicated preparation process, which is not conducive to popularization and convert

Method used

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  • A magnetic resonance imaging contrast agent based on nano-graphene oxide and its preparation method
  • A magnetic resonance imaging contrast agent based on nano-graphene oxide and its preparation method
  • A magnetic resonance imaging contrast agent based on nano-graphene oxide and its preparation method

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Embodiment 1

[0027] Mix and grind 1g graphite powder with 60g NaCl, wash and filter with water to remove NaCl; ultrasonicate the expanded graphite powder with 3:1 concentrated sulfuric acid and concentrated nitric acid for 20h, then measure 23mL 98%wt concentrated sulfuric acid into the reaction vessel, and stir for 8h , then add 5g of potassium permanganate at a rate of 0.1g / min, and stir the reaction; the mixture is stirred at 40°C for 0.5h, heated to 70°C, stirred for 45min, then added with 3mL of deionized water, and stirred at 105°C for 5min; Add 40mL of deionized water and stir at 100°C for 15min; then add 140mL of distilled water and 10mL of 30% hydrogen peroxide solution and stir for 1h; centrifuge repeatedly with 5%wt hydrochloric acid and distilled water to obtain nano-graphene oxide, which is dispersed in water by ultrasonic , the solution color is brown.

Embodiment 2

[0029] Sonicate 5ml graphene oxide for 1h, add 1.2g NaOH, 1.0g chloroacetic acid and sonicate for 3h, convert the hydroxyl group into carboxyl group, and generate graphene oxide containing carboxyl group; then add branched-chain polyethylene glycol (10mg / mL), corresponding EDC, NHS, generate functionalized nanographene oxide pGO, add water to dilute, centrifuge and wash, and finally disperse in water to prepare functionalized nanographene oxide pGO solution;

[0030] After testing, the results show that the pGO aqueous solution is uniform and stable, indicating that it has good dispersion and biocompatibility, and has a long circulation time in the body and a long imaging time window, which is beneficial for in vivo application and imaging diagnosis.

Embodiment 3

[0032] 600mg GdCl 3 Dissolve in 2 mL of deionized water, slowly add to 1 ml of pGO solution (1 mg / mL), and stir overnight at room temperature to obtain the mixture; transfer the obtained mixture to a 50K Da MWCO ultrafiltration centrifuge tube for centrifugation and washing, and dilute the centrifuged product to 1 ml. The MRI contrast agent pGO-Gd is prepared; especially pGO as a nano-carrier material has a large specific surface area and a high loading capacity of gadolinium ions, so the relaxation rate is higher, and the MR image quality is higher and clearer;

[0033] In the preparation process, there is no need to introduce chelating agents such as DTPA into the pGO system for further complex modification, and this step is omitted, which makes the preparation of the contrast agent easier and is conducive to popularization and commercialization.

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Abstract

The invention belongs to the technical field of radiodiagnostics, and relates to a nanometer graphene oxide-based magnetic resonance imaging contrast agent and a preparation method and an application thereof, in particular to a magnetic resonance imaging MRI contrast agent pGO-Gd which is directly prepared from gadolinium ions (Gd<3+>) and branched polyethylene glycol functionalized nano graphene oxide (pGO). The contrast agent is prepared by directly chelating O and N atoms in a nano carrier material pGO system and the gadolinium ions; chelating agents of DTPA and the like do not need to be additionally introduced; and the contrast agent has high gadolinium ion load rate. The contrast agent can be applied to in vivo angiography, organ imaging, tumor imaging and lymph node imaging through intravenous injection and skin injection; the contrast agent has the advantages of being simple in preparation, high in magnetic relaxation rate, good in dispersity and compatibility, long in in-vivo circulation time, long in imaging time window, wide in application and the like and is beneficial to conversion and popularization.

Description

technical field [0001] The invention belongs to the technical field of radiodiagnostics, relates to magnetic resonance imaging and a contrast agent thereof, in particular to a magnetic resonance imaging contrast agent based on nanometer graphene oxide and its preparation method and application. Background technique [0002] The prior art discloses that Magnetic Resonance Imaging (MRI) is a non-invasive medical imaging technology with high resolution and can simultaneously obtain anatomical and physiological information, which is beneficial to disease diagnosis in a living state. This technology is widely used in The clinical radiological diagnosis of tumors and nervous system diseases is also an important research direction of molecular imaging. In clinical practice, in order to obtain high-quality MR images, MRI often needs to inject contrast agents to perform enhanced MRI to improve image contrast. Gd-DTPA and other gadolinium ions (Gd 3+ ) chelate is currently a commonl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/08A61K49/18
CPCA61K49/08A61K49/186
Inventor 王明伟周晓宝曹天野章英剑
Owner FUDAN UNIV SHANGHAI CANCER CENT
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