Staphylococcus drug sensitive strip and preparation method thereof

A staphylococcus, slat technology, applied in biochemical equipment and methods, microorganism-based methods, biochemical instruments, etc., can solve the problem of few types of antibiotics, the design of antibiotic concentration cannot reflect the latest clinical drug information, and the type of antibiotics cannot be adapted to clinical strains drug resistance and other issues, to achieve great application value and reduce the effect of breeding

Inactive Publication Date: 2016-11-23
SHANGHAI FOSUN LONG MARCH MEDICAL SCI CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the staphylococcal susceptibility panel of this patent has few types of antibiotics in clinical use, cannot provide sufficient medication information, and cannot meet the latest medicati

Method used

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  • Staphylococcus drug sensitive strip and preparation method thereof
  • Staphylococcus drug sensitive strip and preparation method thereof
  • Staphylococcus drug sensitive strip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Production of staphylococcal drug-sensitive lath products:

[0050] (1) Configure carnosol aqueous solution

[0051] Weigh 0.4g carnosol and dissolve it in 2000mL distilled water.

[0052] (2) Sterilize the prepared carnosol aqueous solution at 121°C for 15 minutes, and then distribute it into 96 sterilized test tubes, each with 15 mL.

[0053] (3) Prepare each antibiotic stock solution.

[0054] Prepare the stock solution of each antibiotic according to the concentration in the table below:

[0055]

[0056] (4) Configure each well of antibiotic solution

[0057] According to the following requirements, add the antibiotic stock or dilution into the 15 mL test tube containing carnosol aqueous solution to be used.

[0058]

[0059]

[0060]

[0061]

[0062]

[0063] (5) Add sample and split slats

[0064] Load the configured 96 test tubes on the sampler, use the sampler to add samples to the designated staphylococcal drug sensitivity plate, add 50μL to each well;

[0065] (6) Put the ...

Embodiment 2

[0070] The performance test of staphylococcal drug susceptibility board comes from CLSI's broth dilution method.

[0071] (1) Strain recovery

[0072] Kaikai quality control strain Staphylococcus aureus (ATCC 29213) (purchased from Beijing Baote Medical Equipment Co., Ltd. ATCC29213 first generation strain), made a bacterial suspension with 0.9% saline, and inoculated an appropriate amount into nutrient agar medium Incubate at 35°C for 18-24 hours.

[0073] (2) Subculture

[0074] Take an appropriate amount of the resuscitated culture to inoculate the nutrient agar medium slant and incubate at 35°C for 20 hours.

[0075] (3) Strain enrichment culture

[0076] Inoculate the above-mentioned fresh culture after passage to nutrient agar medium and cultivate at 35°C for 20 hours.

[0077] (4) Preparation of bacterial suspension

[0078] The fresh culture of Staphylococcus aureus after the above-mentioned enrichment culture is diluted with 0.9% sterile sodium chloride solution, and after turbid...

Embodiment 3

[0091] Staphylococcal drug sensitivity plate performance test

[0092] (1) Strain recovery

[0093] Open the quality control strain Enterococcus faecalis (ATCC 29212) (same as above), make a bacterial suspension with 0.9% saline, and inoculate an appropriate amount into nutrient agar medium, cultivate at 35°C for 18-24 hours.

[0094] (2) Subculture

[0095] An appropriate amount of the resuscitated culture was inoculated onto the nutrient agar medium slope, and cultivated at 35°C for 22 hours.

[0096] (3) Strain enrichment culture

[0097] Inoculate the above-mentioned fresh culture after passage to nutrient agar medium and cultivate at 35°C for 22 hours.

[0098] (4) Preparation of bacterial suspension

[0099] The fresh culture of Enterococcus faecalis after the above-mentioned enrichment culture is diluted with 0.9% sterile sodium chloride solution, and after turbidity, it is prepared into a bacterial suspension with a turbidity of 0.5 McDonnell standard.

[0100] (5) Broth dilution

[...

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Abstract

The invention discloses a staphylococcus drug sensitive strip which is prepared by the following steps: 1) preparing carnosol water solution; 2) sub-packaging; 3) preparing antibiotic stock solution; 4) sub-packaging liquid materials; 5) adding samples and sub-packaging a strip; 6) drying the strip; 7) performing vacuum packaging; 8) storing the strip. 19 antibiotics are combined on the same detection plate, and MIC (minimal inhibitory concentration) values of the antibiotics are more accurately provided. The antibiotics include gentamicin, rifampicin, erythrocin, clindamycin, compound sulfonamides, vancomycin, penicillin, oxacillin, ampicillin, minocycline, linezolid, gatifloxacin, levofloxacin, cefoxitin, daptomycin, tigecycline, macrodantin, streptomycin and erythrocin/clindamycin. Breeding of drug-resistant strains can be decreased, and drug use of patients is more effectively instructed. The staphylococcus drug sensitive strip functions in monitoring special drug-resistant strain variation and disease prevention and treatment and has an important clinical guiding significance and a great application value.

Description

Technical field [0001] The present invention relates to biological agents. Specifically, it relates to a staphylococcal drug sensitive slab and a preparation method thereof. Background technique [0002] In the past 20 years, due to the widespread use of a wide range of antibacterial drugs in clinical practice, the drug resistance of bacteria has become more and more common. Some bacteria can be resistant to 2-7 drugs at the same time. The wide spread of drug-resistant strains has brought clinical treatment. The great difficulty, coupled with the continuous updating of clinical diagnosis and treatment methods, has improved the diagnosis and treatment effect, but also brought more opportunities for cross-infection, which made the body's immune status tend to be lower. What is particularly striking is that the types of infections have undergone great changes and are still undergoing changes, which puts forward higher requirements for clinical microbial susceptibility testing. The...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12Q1/20C12R1/44
CPCC12Q1/20
Inventor 徐英春杨启文张小江肖盟王龙孙卫兵
Owner SHANGHAI FOSUN LONG MARCH MEDICAL SCI CO LTD
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