A strain of Rhodobacter sphaeroides with antioxidant activity and preparation method and application thereof

A technology of Rhodobacter sphaeroides and fungal agents, applied in the field of microorganisms, can solve problems such as insufficient research and loss of activity, achieve strong oxidation resistance, reduce production costs, and reduce production links

Inactive Publication Date: 2019-09-17
BEIJING UNION UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the activities of carotenoids, coenzyme Q10, and SOD will inevitably be lost in the extraction process. There are not many studies on how to directly use live Rhodobacter sphaeroides to obtain these three biologically active substances at the same time, especially whether Rhodobacter sphaeroids is suitable for extraction. It has not been reported for human consumption as food or health food

Method used

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  • A strain of Rhodobacter sphaeroides with antioxidant activity and preparation method and application thereof
  • A strain of Rhodobacter sphaeroides with antioxidant activity and preparation method and application thereof
  • A strain of Rhodobacter sphaeroides with antioxidant activity and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 prepares Rhodobacter sphaeroides living bacteria agent

[0033] 1 strain activation

[0034] Configure solid medium (mass percentage, the same below): 2% glucose, 1% tryptone, 1% yeast extract powder, 0.5% sodium chloride, 2% agar, the balance is water, pH8.5; and Place it and the petri dish at 121°C for 20 minutes to be sterilized by high-pressure steam; then, under aseptic conditions, invert the plate and pick Rhodobacter sphaeroides to streak the plate; place the plate at 37°C for 3-5 days;

[0035] Configure the slant medium, which is the same as the above-mentioned solid medium, and sterilize at 121 ° C for 20 minutes by high-pressure steam; under sterile conditions, pick a single colony of Rhodobacter sphaeroides on the above plate for slant inoculation; then place the slant Culture at 37°C for 3-5 days;

[0036] Class 2 Rhodobacter sphaeroides fermentation

[0037] 2.1 Seed culture:

[0038] Seed medium: 2% glucose, 1% tryptone, 1% yeast extract ...

Embodiment 2

[0049] Example 2 Determination of in vitro antioxidant activity of Rhodobacter sphaeroides fermented liquid and live bacteria preparation

[0050] The fermentation broth and live bacteria suspension obtained in Example 1 were detected, and the detection indicators included: DPPH free radical scavenging rate, reducing activity, hydroxyl free radical scavenging ability, metal ion chelating ability, and anti-lipid peroxidation ability. Excel2003 and SPSS12.0 statistical software were used to process the experimental data, and each experiment was repeated 3 times.

[0051] 1 DPPH free radical scavenging rate

[0052] The diphenylpicryl radical (DPPH·) scavenging method is an important indicator for the determination of antioxidant capacity, and is widely used in the determination of the free radical scavenging capacity of antioxidant substances. Its principle is based on the electron-donating ability of antioxidant substances. DPPH free radical is a compound with stable unpaired ...

Embodiment 3

[0097] The effect of embodiment 3 Rhodobacter sphaeroides living bacteria agent on the antioxidant activity of Caco-2 cells

[0098] Caco-2 cells are human colon cancer cells purchased from Peking Union Medical College Cell Resource Center.

[0099] Anti-superoxide anion assay kit, hydroxyl radical inhibition kit, LDH assay kit (microplate method), total antioxidant capacity (T-AOC) assay kit, catalase (CAT) assay kit , Superoxide Dismutase (SOD) Activity Assay Kit, Glutathione Peroxidase (GSH-PX) Activity Assay Kit were purchased from Nanjing Jiancheng Bioengineering Institute.

[0100] The Caco-2 cell model is a human cloned colon adenocarcinoma cell, which is similar in structure and function to differentiated small intestinal epithelial cells, has microvilli and other structures, and contains enzymes related to small intestinal brush border epithelium. The Caco-2 cell model has become a standard in vitro screening tool for predicting human intestinal absorption of drugs a...

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Abstract

The invention provides a strain of Rhodobacter sphaeroides with antioxidant activity and the preparation method and application thereof. The classification name of the Rhodobacter sphaeroides is: Rhodobacter sphaeroides WLC11, and the depository unit: China Microorganisms General Microbiology Center of the Culture Collection Management Committee, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, date of deposit: November 27, 2013, deposit number: CGMCC No.8513. The live rhodobacter sphaeroides agent has strong oxidation resistance and can be used to make health food.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a rhodobacter sphaeroides preparation and a preparation method thereof, in particular to a preparation method and application of a living rhodobacter sphaeroides with antioxidant activity and a bacterial preparation thereof. Background technique [0002] Photosynthetic bacteria are an important group of microorganisms in nature. Because of their rich nutritional and medicinal ingredients and unique biotransformation functions, they have increasingly attracted widespread attention from scholars at home and abroad. Their application in the field of health food is one of the focuses of general attention. At present, photosynthetic bacteria have been used in health food in foreign countries, and it can be seen that the application of photosynthetic bacteria in health food has a certain foundation. [0003] Rhodobacter sphaeroides is a purple sulfur-free photosynthetic bacterium, a Gram-ne...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135C12R1/01
CPCC12N1/20C12N1/205C12R2001/01
Inventor 李祖明
Owner BEIJING UNION UNIVERSITY
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