A strain of Pseudogrignon pallidum and its application in the control of plant parasitic nematodes

A technology of Pseudomonas pallidum and nematodes, which is applied to Pseudogrignon pallidum and its application in the control of plant parasitic nematodes, achieving good application and development prospects, remarkable nematode killing effect and stable effect

Active Publication Date: 2019-08-06
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides Pseudogrignon pallidum with high-efficiency nematicidal activity and its application, which solves the problems caused by chemical nematicides on the environment and food safety. The bacterial strain can efficiently kill plant parasitic nematodes, and its The biocontrol microbial agent prepared by fermented bacteria as the active ingredient can also promote the growth of plants

Method used

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  • A strain of Pseudogrignon pallidum and its application in the control of plant parasitic nematodes
  • A strain of Pseudogrignon pallidum and its application in the control of plant parasitic nematodes
  • A strain of Pseudogrignon pallidum and its application in the control of plant parasitic nematodes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Isolation, purification, identification and preservation of O.pseudogrignonense NC1

[0026] The Pseudomonas pallidum NC1 of the present invention is isolated from the rhizosphere soil of a healthy tomato plant in a vegetable field in Hongyuzhuang, Tongjing Town, Yinan County, Shandong Province. Put the fresh tomato root system into the car refrigerator for temporary storage, and rinse it with sterile water for 3-5 minutes after taking it back to the laboratory to remove the root soil, then cut the root system into 1cm root segments, transfer them to the homogenizer, and add 100mL of sterile water, crushed for 2min, the root crushed solution was serially diluted and evenly spread on the NA medium (beef extract 3.0g, peptone 5.0g, glucose 2.5g, agar 18.0g, sterile water to 1L, pH 7.0), and cultured upside down at 28°C for 48 hours. After a single colony grew, pick it on a fresh NA medium with a sterile toothpick for isolation and purification. The strain obtai...

Embodiment 2

[0030] Example 2. Evaluation of Nematicidal Activity of O.pseudogrignonense NC1

[0031] 1. Culture of plate strains

[0032] The Pseudogrignon pallidum NC1 screened in the above Example 1 was transferred from the slant of the test tube to the NA medium, and cultured at 28° C. for 48 hours to obtain plate strains.

[0033] 2. Liquid fermentation culture

[0034] The components and mass percentages of the liquid fermentation medium are: 0.35% glucose, 1% tryptone, 0.45% yeast powder, preferably 1% sodium chloride, dilute to 1L with water, and the stated percentages are all mass percentages , the pH is preferably 7.0.

[0035] Inoculate the plate bacteria obtained in the above step 1 into a 250mL Erlenmeyer flask filled with 30mL of liquid medium, and culture at 28°C for 12h at 180rpm / min to obtain a seed solution. Then insert the seed solution into 200mL culture medium according to the inoculum amount of 2%, cultivate it for 48h to obtain the fermentation solution, dilute it...

Embodiment 3

[0057] Example 3, Evaluation of the Control Efficacy of Pseudogrignonense Paleobacterium (O.pseudogrignonense) NC1 Biocontrol Microbial Agents on Meloidogyne incognita (M.incognita)

[0058] 1. Preparation of Pseudogrignon Paleobacterium NC1 biocontrol microbial agent used in the present embodiment

[0059] (A) Fermentation and culture of Pseudogrignon pallidum NC1, the medium formula and fermentation conditions are the same as step 2 in Example 2.

[0060] The culture was centrifuged to obtain concentrated fermented liquid, the centrifugation speed was 5000rpm / min, and the centrifugation time was 20min.

[0061] (B) adding 0.5-1% sodium alginate (W / V) and 0.5-1% (W / V) chitosan to the concentrated fermented liquid;

[0062] (C) Fully mix the mixed carrier of the bacterium liquid and peat soil and bentonite, the mass ratio of peat soil and bentonite is 9-9.5:0.5-1, and obtain a solid; the mixed carrier of the bacterium liquid and the peat soil and bentonite The weight ratio is ...

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Abstract

The invention provides a strain of Ochrobactrum pseudogrignonense NC1 and the application of the biocontrol microbial agent. The bioassay of Meloidogyne incognita eggs and second-instar larvae from fermentation broth of Pseudogrignon pallidum NC1 showed that the strain may inhibit root-knot nematodes incognita through volatile nematicidal active substances in the fermentation broth Egg hatching and activity of second-instar larvae; the test results of applying the biocontrol microbial agent prepared with Pseudogrignon pallidum NC1 as the active ingredient to potted tomatoes showed that the agent could significantly increase the fresh weight of tomato shoots, The control effect against root-knot nematode incognita is 62.5%, which is equivalent to the effect of nematicide 10% thiazophos. The Pseudogrignon pallidum NC1 and the biocontrol microbial bacterial agent of the present invention have good application prospects in the control of vegetable root-knot nematodes.

Description

technical field [0001] The present invention generally relates to the field of microbes, in particular to a strain of Pseudogrignon pallidum and its application in preventing and treating plant parasitic nematodes. Background technique [0002] Root-knot nematode (Meloidogyne spp.) belongs to the genus Meloidogyne spp. and is an important class of soil-borne pathogens. So far, more than 100 species of root-knot nematodes have been recorded, and the common species in China are M.incognita, M.javanica, M.arenaria and northern root-knot nematodes. Knot nematode (M. hapla). Root-knot nematodes have a wide distribution area and complex and diverse hosts, involving more than 3000 kinds of plants such as food crops, oil crops, fiber crops, tobacco, tea, fruit trees, vegetables, medicinal materials and flowers. It absorbs plant nutrition through stylets, causing great harm to plant underground roots, aboveground stems, leaves and fruits. It is estimated that plant parasitic nemato...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P5/00A01P21/00C12R1/01
CPCA01N63/10C12N1/205C12R2001/01
Inventor 向梅春刘杏忠武云鹏刘建斌
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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