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Colloidal gold immunochromatography test strip and preparing and use method thereof

A technology of immunochromatographic test paper and colloidal gold, which is applied in the direction of analyzing materials, measuring devices, instruments, etc., can solve the problems of temperature sensitivity and large differences between batches, and achieve the goals of wide detection range, improved specificity, and improved detection efficiency Effect

Active Publication Date: 2017-01-11
广东志道医药科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzyme inhibition method still has problems such as large batch-to-batch variation and temperature sensitivity.

Method used

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  • Colloidal gold immunochromatography test strip and preparing and use method thereof
  • Colloidal gold immunochromatography test strip and preparing and use method thereof
  • Colloidal gold immunochromatography test strip and preparing and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Preparation of general hapten-carrier protein conjugate;

[0035]Take the preparation of DEPB-BSA conjugates as an example: the organophosphorus universal hapten used in the present invention contains a carboxyl group, and the carboxyl group is combined with the amino group in the carrier protein by the carbodiimide method, and the organophosphorus universal The hapten is coupled to a carrier protein. The specific operation is as follows: 0.0667g DEPB, 0.0345g NHS and 0.0575g EDC were weighed, dissolved in 3mL dimethyl sulfoxide (DMSO), and magnetically stirred overnight. Weigh 0.12 g of bovine serum albumin (BSA), and dissolve it in 5 mL of sodium bicarbonate buffer (0.1 mol / L, pH 7.0) to obtain a BSA solution. Under stirring, the EDC solution was slowly added dropwise to the BSA solution, and reacted overnight, and the obtained solution was dialyzed with distilled water at 4°C for 5 days, and the dialysate was changed every 8 hours every day. Finally, ...

Embodiment 2

[0036] Embodiment 2: application embodiment;

[0037] Take vegetable leaves from the market, cut an appropriate amount, grind, add 5% ethanol in PBS buffer solution of pH=7 to vibrate and extract, place and layer. Aspirate the supernatant, add it dropwise to the colloidal gold immunochromatography test strip sample pad containing organophosphorus and carbamate pesticide residues, react for 5-10 minutes, and observe the color development of the detection line and the control line. If both the detection line and the control line are red, the result is negative, indicating that there are no organophosphorus pesticides and carbamate pesticides in the sample; if the detection line is not red, but the control line is red, the result is positive, indicating that Contains organophosphorus pesticides or carbamate pesticides; if the control line does not show red, it means that the test strip is invalid, and a new test strip should be used for testing.

Embodiment 3

[0038] Embodiment 3: sensitivity test;

[0039] 25 kinds of organophosphorus pesticides and 10 kinds of carbamate pesticides are detected with the test strip of the present invention, and each kind of pesticides is prepared in different concentrations. The results showed that fenitrocarb, paraoxon-methyl, fenamiphos, insecticide, malathion, diazinon, trifenfos, pyrimiphos-ethyl, memephos, fenitrothion, monocrotophos Phosphorus, methyl parathion, methyl chlorpyrifos, chlorpyrifos, methamidophos, phorate, dimethoate, omethoate, acephate, dicrotofos, parathion, demeton, dichlorvos, terbutyl The test results of 25 organophosphorus pesticides such as thion and amphetamine were all positive, and the minimum detection limit was between 0.1mg / kg and 1mg / kg. Among the 10 kinds of carbamate pesticides, pirimicarb, carbofuran, aldicarb, methomyl, carbaryl, methiocarb, propoxur, isoprocarb, carbofuran, and methomyl 10 All the test results were positive, and the minimum detection limit w...

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Abstract

The invention discloses a colloidal gold immunochromatography test strip and a preparing and use method thereof. This test strip includes PVC floor plate, sample pad, gold labeling pad, detection line, control line, nitrotoluene cellulose membrane and absorbing pad; the described gold labeling pad is coated with a mixture coating of organophosphorus pesticide and carbamates general antibody with the colloidal gold labeling; and the detection line is coated with a conjugate coating of organophosphorus pesticide general hapten and carrier protein, as well as conjugate of carbamates general hapten and carrier protein; and the described control line is coated with the rabbit anti-mouse or goat anti-mouse IgG antibody. The test strip can detect most of the organophosphorus pesticide and carbamates pesticide, with high sensitivity, good stability, and may be used for fast detection and preliminary screening of organophosphorus pesticide and carbamates pesticide residues in the agricultural products such as vegetables and fruits etc.

Description

technical field [0001] The invention relates to the field of pesticide detection, in particular to a colloidal gold immunochromatographic test strip and a preparation and use method thereof. Background technique [0002] Organophosphorus pesticides and carbamate pesticides are still widely used at present, and their detection rate is high, the excess amount is large, and the harm to human body is great. In recent years, incidents of acute poisoning caused by eating vegetables contaminated by organophosphorus and carbamate pesticides have occurred frequently. Therefore, strengthening market supervision and developing rapid detection technology for pesticide residues is a major issue related to the national economy and people's livelihood. [0003] At present, the rapid detection technologies of pesticide residues mainly include chemical reaction method, cholinesterase inhibition method, luminescent bacteria detection method, chemiluminescence technology, biosensing technolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/531G01N33/543
CPCG01N33/531G01N33/54306G01N33/558
Inventor 冯锐龙飞杨鹏博杨松林罗莉妮
Owner 广东志道医药科技有限公司
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