Coupling process for preparing biodiesel and enriching polyunsaturated fatty acid esters

A fatty acid ester and biodiesel technology, applied in biofuels, fermentation, etc., can solve the problems of high energy consumption, high cost, and poor selectivity in the process, and achieve the effects of high product yield, strong applicability, and environmentally friendly and clean process

Inactive Publication Date: 2017-02-15
北京启迪德清生物能源科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the urea inclusion method is more commonly used. In organic solvents, urea can form urea inclusion complexes with straight-chain saturated fatty acids and crystallize at low temperatures. However, this process requires the use of a large amount of organic solvents, which adds subsequent purification steps; Vaporization of the separated matter at low temperature, strictly controlling the temperature to obtain fractions at different temperatures, can obtain higher ω-3PUFAs, but the process consumes a lot of energy; in the anion complexation method, silver nitrate anion can be complexed with ω-3PUFAs, and the product is hydrophilic Therefore, ω-3PUFAs can enter the water phase in the form of anion complexes to ac

Method used

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  • Coupling process for preparing biodiesel and enriching polyunsaturated fatty acid esters

Examples

Experimental program
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Effect test

Embodiment 1

[0022] 10g Botryococcus sp. Microalgae oil (containing eicosapentaenoic acid and docosahexaenoic acid, water based on 10% of oil quality and 200 standard enzyme activities (200U / g soybean oil) based on unit oil quality The liquid lipase derived from Candida antarctica (Candida antarctica) is placed in a one-stage or multi-stage enzyme reactor suitable for enzyme catalysis. The temperature is controlled at 35°C, and then the ethanol based on the molar ratio of oil and fat is 4.5:1 in Add at a constant speed within 3 hours. After 6 hours of reaction, the fatty acid carbon chain length is that the oil of C14-C18 is converted into the corresponding fatty acid ethyl ester (92% conversion rate), and then the reaction solution is phase-separated to separate the enzyme-containing Heavy phase and light phase (oil phase). The heavy phase further utilizes membrane separation to reclaim enzyme protein, selects the organic membrane that molecular weight cut off is 15000 to carry out the rec...

Embodiment 2

[0024] 10g of C.vulgaris microalgae oil (containing eicosapentaenoic acid), 5% water based on oil quality and liquid lipase derived from Aspergillus oryzae (Aspergillus oryzae) based on 200 standard enzyme activities per unit oil quality, Placed in a one-stage or multi-stage enzyme reactor suitable for enzyme catalysis. Control the temperature at 40°C, and then add methanol with a molar ratio of 6:1 based on oil at a uniform rate within 4 hours. After reacting for 8 hours, oils with fatty acid carbon chain lengths of C14-C18 were converted into corresponding fatty acid methyl esters (the conversion rate was 93%). Then the reaction solution is centrifuged to separate the enzyme-containing heavy phase and light phase (oil phase). In the heavy phase, the enzyme protein is further recovered by membrane separation, and an organic membrane with a molecular weight cut-off of 15,000 is selected for the recovery of the above-mentioned lipase. The recovery rate of the enzyme protein is...

Embodiment 3

[0026] 10g fish oil (containing arachidonic acid, C20:4) is based on the water of 2% of oil quality and based on the liquid lipase derived from Thermomyces lanuginosus (Thermomyces lanuginosus) of 200 standard enzyme activities based on unit oil quality and based on unit The liquid lipase derived from Aspergillus oryzae (Aspergillus oryzae) with an oil quality of 200 standard enzyme activities is placed in a one-stage or multi-stage enzyme reactor suitable for enzyme catalysis. Control the temperature at 45°C, and then add ethanol with a molar ratio of 6:1 based on oil at a constant speed within 2 hours. Reaction 5 hours, fatty acid carbon chain length is that the oil of C14-C18 is converted into corresponding fatty acid ethyl ester (93% of conversion rate), then reaction solution is carried out centrifugation, separates the heavy phase and light phase (oily phase) containing enzyme Mutually). In the heavy phase, the enzyme protein is further recovered by membrane separation,...

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Abstract

The invention relates to a coupling process for preparing biodiesel and enriching polyunsaturated fatty acid esters. The coupling process comprises the following steps: reacting grease, short-chain alcohol, water and liquid lipase in a reactor, and then dividing reaction liquid into a heavy phase and a light phase; recycling and reusing enzyme in the heavy phase; and distilling the light phase so as to separate out a C14-C18 fatty acid ester which serves as the biodiesel. A tower oil phase is applied to subsequent immobilized enzyme transformation, and meanwhile, online dehydration is introduced to a reaction course. The process, without conducting pre-treatment on the grease raw material in a previous reaction process of liquid enzyme catalysis, can achieve the effective transformation of the grease, which is C14-C18 in fatty acid carbon-chain length, into corresponding fatty acid ester, with a transformation rate reaching 90% or above; and tower liquid is applied to immobilized lipase catalysis in the second stage, and polyunsaturated fatty acid esters higher than C19 are effectively transformed into corresponding fatty acid ester, with a transformation rate reaching 98% or above. The process has the advantages of being strong in grease raw material adaptability, environment-friendly and clean in process, high in product yield and the like.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and in particular relates to a coupling process of biodiesel preparation and polyunsaturated fatty acid ester enrichment. Background technique [0002] Polyunsaturated fatty acids (PUFAs) refer to long-chain fatty acids with more than one double bond in the molecule. They are essential fatty acids for the human body and need to be ingested from food. According to the position of the PUFA double bond, it is divided into ω-3 and ω-6 series, counting from the carbon atoms of the methyl segment farthest from the carboxyl group in the fatty acid molecule, the first double bond appears at the third and fourth carbon Between atoms is called ω-3PUFA, such as α-linolenic acid (C18:3), docosahexaenoic acid (DHA, C22:6), eicosapentaenoic acid (EPA, C20:5), etc. ; The first double bond between the sixth and seventh carbon atoms is called ω-6PUFA, such as linoleic acid (C18:2) and arachidonic acid (C20:4)...

Claims

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Application Information

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IPC IPC(8): C12P7/64
CPCC12P7/6436C12P7/649Y02E50/10
Inventor 杜伟戴玲妹刘德华
Owner 北京启迪德清生物能源科技有限公司
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