Fermentation promoter for improving output of gentamycin C1a, and adding method thereof

Abstract

The invention relates to a fermentation promoter for improving the output of gentamycin C1a, and an adding method thereof, and belongs to the technical field of fermentation engineering. The fermentation promoter comprises, by mass, 20-50% of D-xylose, 10-30% of N-acetylglucosamine, 1-5% of ammonium ferrous sulfate, 5-10% of betaine, 0.01-0.05% of 2-deoxystreptamine, and the balance of deionized water. 0.001-0.005% Of the fermentation promoter is added to a fermentation medium or an additional medium, and then participates in the fermentation process in a single or combined form. The fermentation promoter optimizes the fermentation environment, promotes metabolism of thalluses, is simply and effectively added, enhances the thallus fermentation performance, and improves the output by 46%.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, specifically relates to the fermentation production of gentamicin, in particular to a fermentation accelerator and an adding method for increasing the yield of gentamicin C1a. Background technique [0002] Gentamicin C1a, as one of the main components of gentamicin, is an important aminoglycoside antibiotic and the mother nucleus of a semi-synthetic new drug ictimicin, which is the first in China and has independent intellectual property rights. The traditional method of preparing C1a is to use resin separation technology to extract C1a single component from multi-component gentamycin. Due to the similar structure and similar physical and chemical properties of each component, the extraction cost remains high, and the high cost in the market The price of purity C1a remains high. Therefore, it is of great significance to study the fermentation of gentamicin C1a. [0003] During ...

AI Technical Summary

Problems solved by technology

In the invention patent (CN95102511) "a method for producing gentamicin", it is disclosed that an appropriate amount of precursor substances are added to the medium to shorten the fermentation cycle and increase the yield. Regarding the precursor substances, three types are mainly disclosed Amino acids (methionine, lysine and arginine), although the methyl group in methionine can be incorporated into the gentamicin molecule, but it cannot change the balance point of the enzyme reaction in the organism, and the result is not obvious for the increase in potency

Some precursors will be transferred to primary metabolism, which is obviously unfavorable for the synthesis of gentamicin by secondary metabolism

Experiments have shown that when methionine is added in excess, biosynthesis turns to basal metabolism, lecithin is synthesized, and even cytokinin is synthesized, resulting in a sharp drop in the production of antibiotics

[0004] Since gentamicin C1a medium has used organic nitrogen sources to form various amino acids after proteolysis, the effect of simply adding amino acid precursors is not obvious enough, but it will increase the risk of switching from secondary metabolism to primary metabolism

[0005] For a long time, how to effectively improve the production of gentamicin C1a has always been a difficult problem in the production of gentamicin. People have also done a lot of research, trying to explain theoretically the interaction between the components in the fermentation process of gentamicin. Transformation mechanism and how to effectively control the content of C1a, but due to the complex factors affecting the components during the fermentation process, even if the large process remains unchanged, any small process fluctuations will have a certain impact on the components

Therefore, so far, there is no clear and complete explanation

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