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Carrier for controlled release of resveratrol and/or resveratrol derivatives, preparation method and application thereof

A technology of resveratrol and its derivatives, which is applied in the fields of prosthesis, tissue regeneration, medical science, etc., can solve the problems of unsatisfactory substitute products, high production costs, and improved comprehensive effects, so as to improve vascularization and The degree of endothelialization, the simplification of the preparation process, and the effect of reducing production costs

Active Publication Date: 2020-01-31
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vascular bypass surgery can be used to treat ischemic diseases. The artificial blood vessel substitutes required in vascular bypass surgery have a great demand in coronary artery bypass surgery, renal dialysis patients and neonatal congenital diseases, but so far there is no satisfactory result. The main reason is that acute thrombus will form after artificial blood vessel implantation, and intimal hyperplasia or calcification will occur in the medium and long term after implantation.
Utilizing VEGF, HGF, NGF and other factors can promote rapid endothelialization of blood vessels; pre-planting cells including endothelial cells (ECs), mesenchymal stem cells (MSCs) and iPS-ECs on artificial blood vessels to construct tissue engineering blood vessels; eNOs) loaded into the scaffold material to achieve local transfection of peripheral cells, so that it releases active molecules that promote angiogenesis such as NO, and finally promotes the regeneration of endothelial cells. These methods can improve the above adverse consequences to a certain extent, but the preparation process is complicated and repeated Poor stability, poor stability of active substances, and high production costs, the overall effect of the stent carrier implantation also needs to be improved

Method used

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  • Carrier for controlled release of resveratrol and/or resveratrol derivatives, preparation method and application thereof
  • Carrier for controlled release of resveratrol and/or resveratrol derivatives, preparation method and application thereof
  • Carrier for controlled release of resveratrol and/or resveratrol derivatives, preparation method and application thereof

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preparation example Construction

[0023] The present invention provides a method for preparing a carrier for controlled release of resveratrol and / or resveratrol derivatives, comprising the following steps:

[0024] S1: Add the degradable polymer to the chloroform-methanol solution, mix well, the volume ratio of the mass of the degradable polymer to the chloroform-methanol solution is 1g:(3-10)mL, preferably 1g:(3-5)mL The degradable polymer is preferably polycaprolactone, polylactide, polyglycolic acid, poly(lactide-glycolic acid) copolymer, poly(lactide-caprolactone) copolymer, polyhydroxyalkanoate , one or more of polydioxanone; the average relative molecular mass of the degradable polymer is preferably 75,000-85,000; the volume ratio of chloroform and methanol in the chloroform-methanol solution is preferably 10:1.

[0025] S2: Add resveratrol and / or resveratrol derivatives to methanol solution, mix well, the mass ratio of resveratrol and / or resveratrol derivatives to methanol solution is (6.25~25) g: 1 m...

Embodiment 1

[0034] Weigh 2.5g of polycaprolactone polymer with an average relative molecular weight of 80000, add it to 10mL of chloroform-methanol solution, the volume ratio of chloroform and methanol in the chloroform-methanol solution is 10:1, stir and mix evenly; weigh 6.25mg Add resveratrol into 1mL of methanol solution, stir to fully dissolve the resveratrol; mix the above two solutions evenly and put them into a micro-syringe with a diameter of 14.9mm, connect the high-voltage DC power supply and the needle of the micro-syringe Connected, wrap the cylindrical receiver of the electrospinning instrument with aluminum foil, connect the cylindrical receiver of the electrospinning instrument to the ground wire, and place the needle of the micro-injector directly on the center of the cylindrical receiver of the electrospinning instrument. For electrospinning, the distance between the needle of the microsyringe and the center of the cylindrical receiver of the electrospinning instrument is 2...

Embodiment 2

[0036] Weigh 2.5g of polycaprolactone polymer with an average relative molecular weight of 80000, add it to 10mL of chloroform-methanol solution, the volume ratio of chloroform and methanol in the chloroform-methanol solution is 10:1, stir and mix evenly; weigh 6.25mg Add resveratrol into 1mL of methanol solution, stir to fully dissolve the resveratrol; mix the above two solutions evenly and put them into a micro-syringe with a diameter of 14.9mm, connect the high-voltage DC power supply and the needle of the micro-syringe Connected, install a stainless steel cylinder with a diameter of 2 mm on the receiver of the electrospinning instrument, connect the cylindrical receiver of the electrospinning instrument to the ground wire, and point the needle of the microinjector to the cylindrical surface of the electrospinning instrument. The center of the receiver is electrospun, the distance between the needle of the microinjector and the center of the cylindrical receiver of the elect...

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Abstract

The invention relates to a carrier for controllably releasing resveratrol and / or resveratrol derivatives, and a preparation method and application thereof. The preparation method comprises the following steps: uniformly mixing a degradable polymer and chloroform methanol solution; uniformly mixing the resveratrol and / or the resveratrol derivatives and methanol solution; mixing the two solutions uniformly, and filling a microsyringe with the mixed solution, wherein the resveratrol and / or the resveratrol derivatives and the degradable polymer are in the mass ratio of (0.25 to 1)to 100; connecting a high-voltage direct-current power supply with a needle of the microsyringe, and aligning the needle of the microsyringe with the centre of a cylindrical receiver of an electro-spinning instrument for performing electro-spinning; performing vacuum dying to obtain a finished carrier for controllably releasing resveratrol and / or resveratrol derivatives. According to the preparation method for the carrier for controllably releasing resveratrol and / or resveratrol derivatives provided by the invention, the flow of the preparation method can be simplified; the repeatability, the operability and the stability are improved; the production cost is reduced.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a carrier for controllably releasing resveratrol and / or resveratrol derivatives, a preparation method and application thereof. Background technique [0002] Ischemic diseases such as coronary heart disease and peripheral arterial occlusive disease seriously threaten human health, especially for lesions that are mainly caused by diffuse damage to terminal small blood vessels. At present, clinical treatment still cannot achieve satisfactory results. Patients suffering from myocardial ischemia, lower limb ischemia and other ischemic diseases. In the past ten years, according to the mechanism of promoting angiogenesis, various therapeutic methods such as pro-angiogenic factors, transgene therapy and cell therapy have been carried out, and some progress has been made. Vascular bypass surgery can be used to treat ischemic diseases. The artificial blood vessel substitutes ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/50A61L27/54
CPCA61L27/50A61L27/507A61L27/54A61L2300/40A61L2300/412A61L2430/22
Inventor 王志红孔德领吴依璠
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI