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A kind of lactobacillus flexus and its application

A kind of Lactobacillus campylobacter, bacterial agent technology, applied in the field of microorganisms, can solve the problem of difference limitation, drug treatment of allergic individuals, etc.

Active Publication Date: 2019-11-08
中科利君股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are problems such as allergies and individual differences in drug treatment

Method used

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  • A kind of lactobacillus flexus and its application
  • A kind of lactobacillus flexus and its application
  • A kind of lactobacillus flexus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Isolation, screening, purification and identification of Lactobacillus curvatus 5-1.

[0023] 1. Isolation of lactic acid bacteria

[0024] After fully mixing the kimchi purchased from a supermarket in Ningbo City, Zhejiang Province, suck the kimchi juice, dilute it with a 0.9% NaCl sterile aqueous solution to a suitable multiple, spread it on an MRS solid plate, and culture it statically at 30°C for 48 hours to obtain a single colony.

[0025] 2. Screening of lactic acid bacteria strains

[0026] Pick a single colony from the plate and inoculate it in MRS medium (peptone 10g, yeast extract 5g, beef extract 10g, glucose 20g, sodium acetate 5g, diammonium citrate 2g, Tween 80 1mL, magnesium sulfate 0.58g, sulfuric acid Manganese 0.05g, dipotassium hydrogen phosphate 2g, agar 15-17g, add water to 1000mL, adjust pH to 6.2, sterilize at 121°C for 20 minutes.), and culture at 30°C for 24h. The activated strain was subcultured twice with 1% inoculum as the seed medium. Th...

Embodiment 2

[0039] Growth curve of Lactobacillus curvatus 5-1 in MRS medium.

[0040]The activated Lactobacillus curvatus (Lactobacillus curvatus) 5-1 strain was inoculated into the MRS medium with an inoculation amount of 1% and cultured statically at 30°C. Determination of bacterial cell OD at different growth times 600 value and the pH of the culture medium. Measurement results such as figure 1 shown. Lactobacillus curvatus (Lactobacillus curvatus) 5-1 is in the logarithmic growth phase from 5 to 18 hours. During this period, the growth rate of the bacteria is the fastest, and a large amount of lactic acid is produced (the pH of the fermentation broth decreases rapidly). After 18 hours, the bacteria growth entered the plateau stage, at this time the growth rate of the bacteria slowed down and tended to be stable, and the pH of the fermentation broth was maintained at about 4, indicating that the acid production ability of the strain was inhibited after entering the plateau stage. I...

Embodiment 3

[0042] Drug susceptibility profile of Lactobacillus curvatus 5-1.

[0043] The drug susceptibility spectrum of Lactobacillus curvatus 5-1 was determined by antibacterial drug susceptibility paper sheet (Hangzhou Microbial Reagent Co., Ltd.). Spread 200 μL of bacterial culture solution evenly on the MRS solid plate, place 3 pieces of drug-sensitive paper on each plate, and after incubating at 30°C for 48 hours, measure the diameter of the inhibition zone with a vernier caliper. Table 1 shows the susceptibility results of Lactobacillus curvatus 5-1 to different classes of antibiotics. It can be seen that Lactobacillus curvatus (Lactobacillus curvatus) 5-1 showed different sensitivity to different antibiotics, oxacillin, cephalexin, tetracyclines (except minocycline), aminoglycosides, carbapenem Mycelenes, quinolones, glycopeptides, monocyclic lactams, chloramphenicol and rifampicin are relatively insensitive, and the diameter of the inhibition zone is only around the diameter o...

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Abstract

The invention discloses a Lactobacillus curvatus and an application thereof. The strain is named as Lactobacillus curvatus 5-1, and the preservation number is CGMCC No.12891. The strain has the ability to efficiently degrade purine nucleosides, and can tolerate the acidic environment and bile salt concentration under normal human conditions, and can decompose purine nucleosides in food in the intestinal tract, thereby reducing the absorption of purine nucleosides in the small intestine . Therefore, Lactobacillus curvatus 5‑1 is a good strain for the development of probiotics for the auxiliary treatment of hyperuricemia.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a lactobacillus flexus and its application. Background technique [0002] Hyperuricemia is a state of the body in which the concentration of uric acid in the blood exceeds the normal range due to the disorder of purine metabolism in the human body, excessive production of uric acid or decreased excretion of uric acid. Modern medical research has found that hyperuricemia is closely related to the occurrence of gout, hypertension, hyperlipidemia, atherosclerosis, obesity, and insulin resistance. Although hyperuricemia is related to factors such as genes, environment, gender, age, and living and eating habits, the etiology of the disease is relatively complex. The incidence rate is increasing rapidly in our country, and it has become one of the high-incidence diseases of modern people. [0003] The current treatment of hyperuricemia is mainly to take drugs and diet restrictions. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P19/06A23L33/135C12R1/225
CPCA61K2035/115C12N1/20C12N1/205C12R2001/225
Inventor 梅乐和麻菊美赵伟睿胡升黄俊王进波
Owner 中科利君股份有限公司
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