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A kind of Chinese herbal medicine compound microecological preparation and its preparation method and application

A technology of compound micro-ecology and Chinese herbal medicine, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems such as the inability to stimulate the self-immunity of aquatic animals, the single effect of micro-ecological preparations, and the inability to improve water quality, etc. Achieve the effect of improving feed utilization and conversion rate, inhibiting reproduction and growth, and improving immunity

Active Publication Date: 2020-06-12
清远海贝生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on microecological preparations in the prior art can either be used to improve water quality, or can be used to improve the immunity of aquatic animals, and the microecological preparations used to improve the immunity of aquatic animals basically rely on adding them to the feed. The way of feeding, for example, discloses " multi-functional sea cucumber cultivation compound microecological preparation " in the open text of patent application number CN201010602297.0, and this microecological preparation adopts Bacillus atrophaeus, Bacillus subtilis and Bacillus cereus, The preparation is added to the feed for feeding, which has antibacterial, immune, growth-promoting and other effects, but does not have the effect of improving water quality; the publication of the patent application number CN20140461391.7 discloses "a compound microecological preparation and its preparation method", the micro-ecological preparation adopts Bacillus subtilis, Bacillus megaterium, Bacillus amyloliquefaciens, Bacillus licheniformis, Enterococcus faecium, Lactobacillus pumilus, Lactobacillus plantarum, Lactococcus lactis and Saccharomyces cerevisiae, etc., can be used It is used to degrade harmful substances such as ammonia nitrogen and hydrogen sulfide in the water body to improve water quality, but it cannot stimulate the immunity of aquatic animals; in the publication of the patent application number CN201310210210.09, "a Chinese herbal medicine feed additive for improving the immunity of aquatic animals" is disclosed. This Chinese herbal medicine additive is used by adding it to the feed. This additive can only promote the immunity of aquatic animals, and has no effect on improving the water quality of aquaculture.
These microecological preparations have a single effect, and the method of use is also limited, it is not easy to popularize and use, and the economic value is limited

Method used

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  • A kind of Chinese herbal medicine compound microecological preparation and its preparation method and application

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preparation example Construction

[0047] As a preference of the present invention, the step (1) in the above preparation method comprises: the raw materials of the Chinese herbal medicine extract including astragalus, mugwort leaves, cassia seeds, angelica, and Eucommia are pulverized and uniformly mixed, and then added 3-11 times (for example, 4 times) volume of water for 8-10 hours, decocting at 100-110°C for 1-2 hours (for example, 2 hours), separate and filter to obtain filter residue and filtrate, add 3-4 times the volume of water to the filter residue (for example, 4 times the volume water), then decocted at 100-110° C. for 1-2 hours (for example, 1 hour), and then filtered and separated to obtain the filtrate of the first repeated decoction and the filter residue after the first repeated decoction, and then to the first time. Add 3-4 times the volume of water (for example, 3 times the volume of water) to the filter residue after repeated decoction, decoct at 100-110°C for 0.5-1 hour (for example, 0.5 hou...

Embodiment 1

[0081] A preparation of Chinese herbal medicine compound probiotics for aquaculture, the specific steps are as follows:

[0082] (1) Preparation of Chinese herbal medicine extract: 30-35% astragalus, 10-15% Folium Artemisiae Argyi, 15-20% Semen Cassiae, 15-20% Angelica sinensis, 10-15% Eucommia ulmoides as raw materials, after the raw materials are pulverized and mixed uniformly, add Soak in 4 times of water for 9 hours, decoct at 105°C for 2 hours, separate and filter, add 4 times of water to the filter residue, decoct for 1 hour, filter and separate again, add 3 times of water to the separated filter residue, decoct for 0.5 hours, separate and filter, and mix the filtrate from the three times of filtration , which is the Chinese herbal medicine extract;

[0083] (2) Preparation of composite bacteria liquid:

[0084] ① Strain activation and rejuvenation: Inoculate the inclined-plane strains of Lactobacillus, Bacillus subtilis, yeast, and photosynthetic bacteria under aseptic...

Embodiment 2

[0092] A kind of preparation of Chinese herbal medicine compound microecological preparation for aquaculture, except that the ratio of Chinese herbal medicine is changed, other preparation steps are the same as in Example 1, and the number of live bacteria in the Chinese herbal medicine compound microecological preparation is 1.6×10 9 cfu / mL.

[0093] The raw material components of the Chinese herbal medicine extract are 30% astragalus, 20% mugwort leaves, 20% cassia seeds, 15% angelica and 15% eucommia in terms of mass percentage.

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Abstract

The invention relates to a Chinese herbal medicine compound micro-ecology preparation and a preparation method thereof, and a use in the aquaculture. The micro-ecology preparation is mainly prepared by Chinese herbal medicine extracting solution, a fermentation medium, and compound bacteria solution through the fermentation. The Chinese herbal medicine extracting solution is mainly extracted from 30-35% of astragalus membranaceus, 10-15% of folium artemisiae argyi, 15-20% of semen cassia, 15-20% of Angelica sinensis, and 10-15% of eucommia ulmoides. The compound bacteria solution is prepared by fermenting and mixing lactobacillus, Bacillus subtillis, saccharomycetes and photosynthetic bacteria. The Chinese herbal medicine compound micro-ecology preparation is capable of purifying water quality and improving aquatic livestock immunity in the aquaculture process, rapidly reducing the hazardous substance in the water body, improving the water quality, inhibiting the breeding and growth of pathogenic microorganism, stimulating the own immunity and anti-stress capacity of the aquatic livestock, reducing the occurrence of diseases, improving the survival rate of the aquatic livestock, reducing the death rate, and improving the utilization rate and the conversation rate of feed.

Description

technical field [0001] The invention relates to a Chinese herbal compound microecological preparation, a preparation method thereof, and use in aquaculture. Background technique [0002] In recent years, the demand for aquatic animals has continued to increase, and fishing alone is far from being able to meet the market demand. Therefore, the rapid development of the artificial breeding industry of aquatic animals has been born. [0003] my country is currently the world's largest consumer of aquaculture. With the advancement of aquaculture technology, the large-scale aquaculture industry has developed rapidly and brought more and more economic value. However, with the development of large-scale aquaculture, There are also problems of deterioration of the water environment and frequent occurrence of diseases. At the same time, the abuse of antibiotics and the use of other drugs lead to the imbalance of the normal microbial flora in the gastrointestinal tract of aquatic animal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C02F3/34A23K10/18C12R1/225C12R1/125
CPCC02F3/34C02F3/347C12N1/14C12N1/20
Inventor 王凯王蔚淼齐振雄
Owner 清远海贝生物技术有限公司