Complex microbial inoculant used for degrading waste water rich in surfactant and preparation method thereof

A technology of surfactant and compound bacterial agent, applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the fluctuation of surfactant content, the difficulty of microorganism degradation, and the insignificant treatment effect. And other issues

Inactive Publication Date: 2017-05-31
泰伦特生物工程股份有限公司
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The influent COD of a sewage treatment station in an enterprise is generally 2000-4000mg/L, and the surfactant is 500-2000mg/L. The typical SBR wastewater treatment process is adopted, and the effluent is required to meet the secondary discharge standard. The water quality has always been in a state of fluctuation, and the content of surfactant in wastewater fluctuates greatly with the different products produced, which will have a great impact on the SBR biological system. When the content of surfactant in wastewater When the surfactant is low, the treatment effect is better. Once the surfactant content in the wastewater is high, there is basically no effect, and the whole system collapses, and it will tak

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Complex microbial inoculant used for degrading waste water rich in surfactant and preparation method thereof
  • Complex microbial inoculant used for degrading waste water rich in surfactant and preparation method thereof
  • Complex microbial inoculant used for degrading waste water rich in surfactant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0035] The preparation method of the above-mentioned composite bacterial agent for degrading wastewater rich in surfactants requires independent cultivation of four bacterial powders. Since each bacterial species belongs to Bacillus, the preparation process of each bacterial powder is the same, specifically including the following steps:

[0036] (1) Inoculate a single strain on a slant for cultivation;

[0037] The slant medium is: tryptone 10g / L, yeast extract powder 5g / L, sodium chloride 10g / L, agar powder 15g / L, pH natural, sterilized at 121°C for 20 minutes;

[0038] The cultivation process is as follows: after inoculation, it is placed in a biochemical incubator at 32-35° C. for 24-30 hours.

[0039] (2) Inoculate the product of step (1) into the seed shake flask for cultivation;

[0040] The seed shake flask medium is: tryptone 10g / L, yeast extract powder 5g / L, sodium chloride 10g / L, pH natural, sterilized at 121°C for 20 minutes;

[0041] The cultivation process is a...

Embodiment 1

[0055] Wastewater COD is 3850mg / L, which contains non-ionic surfactant 580mg / L, contains anionic surfactant 1390mg / L, pH value is 8.6, the specific implementation steps are as follows:

[0056] (1), using a 1L measuring cylinder, add 900ml of waste water for later use.

[0057] (2), take Bacillus pumilus (BNCC103925), Bacillus subtilis (BNCC188159), Bacillus cereus (BNCC338099) and Bacillus megaterium (BNCC190686) bacteria powder according to the ratio of 3:3:3:1 to get 10g altogether, then Add it into water containing 2% molasses to make the final volume 100ml, and control the temperature at 32-35°C while stirring continuously for 2-4 hours for later use.

[0058] (3) Add the bacterial solution in (2) into the graduated cylinder of (1) to establish a 1L wastewater degradation simulation system.

[0059] (4) Use an aeration device to continuously aerate (3), and control the dissolved oxygen in the system at 2-4 mg / L.

[0060] (5) After continuously aerating the waste water d...

Embodiment 2

[0065] Wastewater COD is 3054mg / L, which contains anionic surfactant 1792mg / L, pH value is 8.2, the specific implementation steps are as follows:

[0066] (1), using a 1L measuring cylinder, add 900ml of waste water for later use.

[0067] (2), take Bacillus pumilus (BNCC103925), Bacillus subtilis (BNCC188159), Bacillus cereus (BNCC338099) and Bacillus megaterium (BNCC190686) bacteria powder according to the ratio of 1:4:4:1 to get 10g altogether, then Add it into water containing 2% molasses to make the final volume 100ml, and control the temperature at 32-35°C while stirring continuously for 2-4 hours for later use.

[0068] (3) Add the bacterial solution in (2) into the graduated cylinder of (1) to establish a 1L wastewater degradation simulation system.

[0069] (4) Use an aeration device to continuously aerate (3), and control the dissolved oxygen in the system at 2-4 mg / L.

[0070] (5) After continuously aerating the waste water degradation simulation system for 24-30 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a complex microbial inoculant used for degrading waste water rich in surfactant. Four strains, including bacillus pumilus, bacillus subtilis, bacillus cereus and bacillus megatherium, are used and are subjected to slant culture, shake-flask culture, seed tank culture, fermentation tank culture, centrifugation and spray drying processing to obtain finished product bacterial powder of each strain. When the complex microbial inoculant is used, four kinds of bacterial powder are regulated and formed according to a ratio between nonionic surfactant and ionic surfactant in the waste water, then, the bacterial powder is put into a facultative tank and an aeration tank of a waste water biodegradation system; each bacterial powder contains a great quantity of active spores, and the active spores can quickly form a commensalism cooperative system; within 30h, the degradation of non-ions can be 90% or above, the degradation of anionic surfactant can be 99% or above, the integral degradation of COD (Chemical Oxygen Demand) in the waste water can be 95% or above, and discharged water can achieve a secondary discharged standard. The complex microbial inoculant is an efficient sewage processing biological enhancement technology without secondary pollution.

Description

technical field [0001] The invention belongs to the technical field of purification of wastewater rich in surfactants, in particular to a composite bacterial agent for degrading wastewater rich in surfactants and a preparation method thereof. Background technique [0002] Surfactant refers to a substance that can significantly change the interface state of the solution system by adding a small amount. It has fixed hydrophilic and lipophilic groups, which can be oriented on the surface of the solution. Its molecular structure has amphiphilicity: one end is hydrophilic Water group, the other end is a hydrophobic group, and the hydrophilic group is often a polar group, such as carboxylic acid, sulfonic acid, sulfuric acid, amino group or amine group and its salts, hydroxyl group, amide group, ether bond, etc. can also be used as Polar hydrophilic groups, while hydrophobic groups are often non-polar hydrocarbon chains, such as hydrocarbon chains with more than 8 carbon atoms. W...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/125C12R1/085C12R1/11C02F101/30
Inventor 谢攀王晓菲徐静王永芝王洋
Owner 泰伦特生物工程股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap