Uric acid detecting electrochemical sensor, preparation method thereof and application
An electrochemical and sensor technology, applied in the field of electrochemical biosensors, can solve the problems of reducing surface area and electrochemical performance gap, etc., and achieve the effect of increasing specific surface area, low detection limit and avoiding interference
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Embodiment 1
[0037] (1) Preparation of polystyrene / graphene core-shell microspheres
[0038] Preparation of cationic polystyrene microspheres: using dispersion polymerization method, add 5mL styrene and 0.0906g initiator azobisisobutyronitrile into a three-necked flask, stirring speed 200rpm, after 30 minutes of nitrogen, add 0.0906g stabilizer methyl Acryloyloxyethyl trimethyl ammonium chloride, 40 mL methanol and 10 mL deionized water were added to a three-necked flask, heated to 75°C, and kept for 8 hours to obtain a white polystyrene emulsion. Use deionized water and methanol respectively Centrifugal washing, speed 4000rpm, time 30min, vacuum drying the product to obtain white powder cationic polystyrene microspheres.
[0039] Synthesis of Graphene Oxide: Put 0.5g graphite powder, 0.625g sodium nitrate and 23mL concentrated sulfuric acid into a 500mL flask with magnets, put the flask in an ice-salt bath, stir and mix well, then add 3g potassium permanganate Slowly add into the flask in bat...
Embodiment 2
[0047] Make the electrochemical sensor working curve: open the electrochemical workstation, open-pore graphene modified glassy carbon electrode as the working electrode, saturated calomel electrode as the reference electrode, platinum wire as the counter electrode, and set different pH values (5.0, 5.5, 6.0, 6.5, 6.8, 7.0, 8.0) phosphate buffer solution as the base solution, connect the electrochemical workstation, and insert these three electrodes into the uric acid phosphate buffer solution of different pH values, using the electrochemical workstation differential pulse voltammetry Test current-voltage curve. The pH value is determined by comparing the oxidation peak current of uric acid obtained under different pH values. For the electrode of this example, the optimal pH value is 6.8.
Embodiment 3
[0049] The responsiveness of different electrodes to the detection of uric acid: The electrochemical sensor obtained in Example 1 was placed in a phosphate buffer with a pH of 6.8 and a uric acid concentration of 200 μM, and the cyclic voltammetry in an electrochemical workstation was used. The glassy carbon electrode, the graphene modified glassy carbon electrode, and the open-pore graphene modified glassy carbon electrode are used as the working electrode to obtain different cyclic voltammetry curves. image 3 The middle is the comparative cyclic voltammogram of three different electrodes for detecting uric acid. It can be seen that the open-pored graphene modified electrode (curve c) has the largest oxidation current for uric acid, followed by the graphene modified electrode (curve b), bare glassy carbon The electrode (curve a) has the weakest electrochemical responsiveness to uric acid. This may be because open-pore graphene reduces graphene agglomeration due to the pore stru...
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