A kind of magnetic composite microsphere and its preparation method and application

A technology of magnetic composite microspheres and stirring reaction, which is used in the preparation of microspheres, biochemical equipment and methods, magnetic objects, etc., to achieve good reusability, improved temperature stability, improved enzymatic activity recovery ability and temperature stability performance. Effect

Active Publication Date: 2020-06-26
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the magnetic composite material Fe 3 o 4 @SiO 2 - p (GMA - co - NIPAM) preparation and immobilized cellulase, there is no literature report at home and abroad

Method used

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  • A kind of magnetic composite microsphere and its preparation method and application
  • A kind of magnetic composite microsphere and its preparation method and application
  • A kind of magnetic composite microsphere and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Fe 3 o 4 Synthesis

[0044] Dissolve 1.350g of ferric chloride hexahydrate in 70mL of ethylene glycol, add 3.854g of ammonium acetate and 0.400g of trisodium citrate, stir for 1 hour at an oil bath temperature of 170°C until the solution becomes a uniform system, and transfer to a polytetrafluoroethylene lining Heated to 200°C for 16h in a stainless steel reactor. Cool to room temperature after taking out, magnetically separate the product, wash with ethanol until the supernatant is transparent, and dry in a vacuum oven at 50°C until constant weight.

Embodiment 2

[0045] Example 2: Fe 3 o 4 @SiO 2 Synthesis of -p(GMA-co-NIPAM)

[0046] (1) Fe 3 o 4 @SiO 2 - Synthesis of MPS

[0047] The product Fe in the embodiment 1 of 0.1 g 3 o 4 Disperse in 15 mL of a mixed solution of ethanol and water with a volume ratio of 4:1, then add 1 mL of ammonia solution, stir mechanically and slowly add 0.5 mL of TEOS dropwise into the system, and react at room temperature for 6 hours. 0.3mL MPS was added into the system, and the stirring reaction was continued for 12 hours. The product was separated magnetically and washed several times with ethanol. Dry to constant weight in a vacuum oven at 50°C.

[0048] (2) Fe 3 o 4 @SiO 2 Synthesis of -p(GMA-co-NIPAM)

[0049] Take 50mg Fe 3 o 4 @SiO 2 - Add MPS to a one-necked flask, add 40mL acetonitrile for ultrasonic dispersion for 3min, add 50 mgNIPAM, 0.05mL GMA, 100mg cross-linking agent MBA, 4mg initiator AIBN, heat the flask in an oil bath, install a fractionation column, a condenser...

Embodiment 3

[0050] Example 3: Fe 3 o 4 @SiO 2 Synthesis of -p(GMA-co-NIPAM)

[0051] (1) Fe 3 o 4 @SiO 2 - Synthesis of MPS

[0052] The product Fe in the embodiment 1 of 0.5 g 3 o 4 Disperse in 90 mL of a mixed solution of ethanol and water with a volume ratio of 4:1, then add 3 mL of ammonia solution, stir mechanically and slowly add 1.2 mL of TEOS dropwise into the system, and react at room temperature for 24 hours. 1.5 mL of MPS was added to the system, and the stirring reaction was continued for 48 hours. The product was separated magnetically and washed several times with ethanol. Dry to constant weight in a vacuum oven at 50°C.

[0053] (2) Fe 3 o 4 @SiO 2 Synthesis of -p(GMA-co-NIPAM)

[0054] Take 100mg Fe 3 o 4 @SiO 2 - Add 80mL of acetonitrile to ultrasonically disperse MPS for 3 minutes, add 150mg of NIPAM, 0.15mL of GMA, 200mg of cross-linking agent MBA, 10mg of initiator AIBN, heat the flask in an oil bath, install a fractionating column, a condenser...

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Abstract

The invention relates to magnetic composite microspheres, and a preparation method and application thereof, belonging to the technical field of chemical material preparation. The preparation method comprises the following steps: synthesizing Fe3O4@SiO2-MPS (modified polystyrene); mixing, adding acetonitrile, carrying out ultrasonic dispersion, adding isopropyl acrylamide, glycidyl methacrylate, N,N'-methylene-bis acrylamide and azodiisobutyronitrile, mixing, and carrying out oil-bath heating; distilling to obtain part of acetonitrile, and stopping the reaction; carrying out magnetic separation on the obtained, and repeatedly washing with ethanol and water; and drying in a vacuum drying oven to obtain the product. The product is used for cellulase immobilization. The synthesized magnetic composite microspheres have superparamagnetism, can enhance the enzyme activity restorability and heat stability when being used for cellulase immobilization, and implements thermal protection on the cellulase.

Description

technical field [0001] The invention relates to a magnetic composite microsphere and its preparation method and application, in particular to the synthesis of a temperature-sensitive magnetic core-shell microsphere and its application in cellulase immobilization, belonging to the technical field of chemical material preparation. Background technique [0002] One of the main components of plants is cellulose, which accounts for about 35%-50% of the dry weight of plants. Cellulose is the most widely distributed natural carbohydrate in the world. The effective development and utilization of cellulose in plants is of great significance, which can effectively alleviate problems such as energy crisis, environmental pollution, energy saving and emission reduction, and food shortage. At present, the total utilization of cellulose is less than 0.5% of the world's production, and a large number of cellulose resources have not been effectively, rationally and widely used. At present, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/06B01J13/14H01F1/11C12N11/14C12N11/082
CPCB01J13/06B01J13/14C12N9/2437C12N11/08C12N11/14H01F1/112
Inventor 王赟韩娟王蕾倪良李程刘倩戎军辉伍晨
Owner JIANGSU UNIV
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