Double-targeted ternary complex nucleic acid transfer and release system and preparation method thereof

Abstract

The invention belongs to the technical field of biological medicines, and relates to a D type IF-7 polypeptide-modified reductive dendrimer-like macromolecule (RHB) self-assembled double-targeted nucleic acid transfer and release system and a preparation method thereof. The preparation method comprises the following steps of automatically compressing plasmid DNA (deoxyribonucleic acid) by reductive dendrimer-like cationic polymer polyamino ammonia (RHB), so as to form a binary complex; using an inverted sequence D type technique to transform an IF7 peptide of a targeted tumor blood vessel endothelial cell surface specificity receptor Anxal, and synthesizing RIF7 polypeptide; performing amidation reaction to combine the RIF7 polypeptide onto an HA molecular structure of a CD44 receptor on the surface of a targeted tumor cell, and synthesizing a double-targeted coating material; under the electrostatic action, coating the formed RHB / DNA binary complex, so as to form a targeted ternary complex nucleic acid transfer and release system. The preparation method has the advantages that the transfer and release system can effectively compress and coat the plasmid DNA; by utilizing the double-targeted action, the complex can cross tumor blood vessel barriers and tumor cytomembrane barriers step by step, the transfection efficiency of a gene is improved, and the toxicity of a carrier system is decreased.

Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and relates to a non-viral nucleic acid delivery system and a preparation method thereof, in particular to a reducing dendritic macromolecule (RHB) modified by a D-type IF-7 polypeptide. ) self-assembled dual targeting ternary complex nucleic acid delivery system and preparation method thereof. Background technique

[0002] Gene therapy is a hot spot in the field of current tumor therapy research; the key to the application of this technology is the need for a safe and efficient gene delivery carrier system. Compared with viral vectors, non-viral vectors have the characteristics of low toxicity, easy synthesis, low immune response, and targetable modification. Cationic polymer is a kind of non-viral gene delivery carrier that has been researched more at present, commonly used are polyethyleneimine (Polyethyleneimine, PEI), chitosan and its derivatives, polylysine (Poly(L-lysine), PLL), poly...

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