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Kit for determining trace elements in human urine

A technology of trace elements and reagent kits, applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems of complex collection and pretreatment methods, huge differences in organic matter in contents, and increase the difficulty of procurement, etc., to achieve The processing method is fast, the running cycle is short, and the effect of reducing random errors

Inactive Publication Date: 2017-08-08
BIOYONG TECH
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the different sources of samples such as blood, urine, and milk, the organic matter in the content varies greatly, and the interference of various organic matter on trace elements is different. long
When testing one type of sample, it is necessary to purchase three types of kits at the same time, which increases the difficulty of procurement

Method used

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  • Kit for determining trace elements in human urine
  • Kit for determining trace elements in human urine
  • Kit for determining trace elements in human urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Embodiment 1, urine collection and pretreatment

[0089] The urine pretreatment process is as follows: Figure 22 shown.

Embodiment 2

[0090] Embodiment two, the preparation of urine trace element assay kit

[0091] 1. Preparation of diluent

[0092] Add 10mL of original nitric acid, add ultrapure water to make up to 500mL, and mix well to obtain the diluent.

[0093] 2. See Table 1 for the preparation of the mixed standard solution.

[0094] (1) Standard material: single standard liquid calcium, magnesium, iron, copper, zinc, selenium, aluminum, vanadium, chromium, manganese, cobalt, nickel, arsenic, molybdenum, cadmium, tin, antimony, barium, mercury, thallium, lead (1000mg / L, National Nonferrous Metals and Electronic Materials Analysis and Testing Center); potassium iodate (1000mg / L, National Standard Materials Research Center).

[0095] (2) Prepare the winning standard solution: diluent, ultrapure water, standard solutions of 21 single elements, see Table 2.

[0096] Table 1 Preparation method of standard solution

[0097]

[0098] Table 2 Preparation method of the standard solution

[0099] ...

Embodiment 3

[0109] Embodiment three, the impact of nitric acid volume concentration on detection result in diluent

[0110] 1 Instrument and working parameters

[0111] In this paper, Clin-ICP-QMS-I was used for elemental analysis, and the basic parameter settings of the instrument are shown in Table 4.

[0112] Table 4 Instrument conditions of Clin-ICP-QMS-I

[0113] parameter set up RF power 1450w cooling air 14L / min Auxiliary gas 0.95L / min atomizing gas 1.05L / min pump speed 30rpm Spray chamber temperature 2℃ sampling depth 3.68mm scan mode peak jump number of channels 3 dwell time 10ms scan times 10

[0114] Since the collision reaction cell can significantly help to reduce interference and improve the detection limit. Therefore, for calcium Ca, magnesium Mg, tin Sn, antimony Sb, iodine I, barium Ba, mercury 201Hg, thallium 205Tl, lead 208Pb, the standard mode is used; for iron Fe, copper Cu, zin...

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Abstract

The present invention provides a method and a matched kit for measuring trace elements in human urine, the kit comprises a dilution solution, a standard solution containing 21 trace elements, a quality control product, an internal standard storage solution, and the like. The kit has the advantages of simple sample pretreatment, low cost, high accuracy and the precision and good stability, and is suitable for the detection of high throughput samples in clinical. The kit can be used for detection of nutrient elements and toxic elements in the human urine, environmental supervision, occupation metal exposure, environmental toxic trace pollution detection, microstimulant detection, and forensic toxicological detection of human bodies or corpses.

Description

technical field [0001] The invention belongs to the technical field of biological detection and relates to a kit for detecting 21 kinds of trace elements in urine by using inductively coupled plasma mass spectrometry. Background technique [0002] Trace elements in the human body account for about 0.05% of the body weight, and they play a key role in biochemical processes. Trace elements are involved in the functional structure of tissues, cells and sub-cells, including immune regulation of humoral and cellular mechanisms, nerve conduction, muscle contraction, membrane potential law, mitochondrial activity and enzyme reactions, etc. In a sense, trace elements are more important to the matrix than other non-nutrients such as vitamins. The body can produce vitamins by itself, but trace elements must depend on the external environment, and the body cannot manufacture them. For example, essential trace elements are closely related to the health and balance of the human body. A...

Claims

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Application Information

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IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 黎庆陈文举张荣熊婵李大为马庆伟
Owner BIOYONG TECH
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