Keratin biomacromolecular nitric oxide donor and synthesis and application thereof

A biomacromolecule and nitric oxide technology, applied in the field of keratin biomacromolecule nitric oxide donor and its synthesis, can solve the problems of limiting the effectiveness and persistence of biological effects, having biological toxicity, short half-life and the like, Achieve the effect of promoting the growth of vascular endothelial cells, high molecular weight and low toxicity

Active Publication Date: 2017-09-08
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common exogenous small molecule donors of NO have a short half-life in vivo, are unstable, and have biological toxicity, etc., thus limiting the effectiveness and persistence of their biological effects

Method used

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  • Keratin biomacromolecular nitric oxide donor and synthesis and application thereof
  • Keratin biomacromolecular nitric oxide donor and synthesis and application thereof
  • Keratin biomacromolecular nitric oxide donor and synthesis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Keratin biomacromolecule nitric oxide donor and its synthesis (oil phase method)

[0029] Take 0.1 g of laboratory-extracted reduced keratin (KSH, mercaptokeratin) and disperse it with 5 mL of ether in a brown bottle, add 1 mL of ether solution of tert-butyl nitrite, keep it in the dark and avoid light, and stir for 24 hours at room temperature under a nitrogen atmosphere. Rotary evaporation at room temperature removes unreacted tert-butyl nitrite, by-products tert-butanol and ether to obtain S-nitrosylated keratin (KSNO), which is a NO donor keratin.

[0030] The above-prepared S-nitrosylated keratin (KSNO) was made into a solution with a certain concentration, and the ultraviolet absorption spectrum was measured in the wavelength range of 200-600nm, such as image 3 shown. The absorption peak at 334nm is the n on the S-NO bond on the modified keratin 0 -π* electron transition absorption (V.B. Damodaran, L.W. Place, M.J. Kipper, M.M. Reynolds.

[0031]...

Embodiment 2

[0033] Embodiment 2: Keratin biomacromolecule nitric oxide donor and its synthesis (aqueous phase method)

[0034] Put 0.1 g of the reduced keratin sample into 2 mL, 50 mg / mL sodium nitrite aqueous solution and stir to dissolve. Under ice bath, slowly add 5mL of 5M hydrochloric acid dropwise. Avoid light and stir for 2h. The excess small molecules were removed by dialysis, and the SNO terminal keratin was obtained by freeze-drying. Samples were stored at -20°C in the dark.

Embodiment 3

[0035] Embodiment 3: Stability experiment of keratin biomacromolecule nitric oxide donor

[0036] Take 20 mg of the keratin biomacromolecule nitric oxide donor KSNO prepared in Example 1 or 2, dissolve it in 5 ml of phosphate buffer, and dialyze the solution at 37° C. with 35 ml of phosphate buffer containing 0.1 mmol EDTA. Set the time to take samples, and use the NO kit to measure the content of NO. The results are as follows: Figure 5 shown. Depend on Figure 5 It can be seen that the keratin biomacromolecule nitric oxide donor has good stability in phosphate buffer solution.

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Abstract

The invention discloses a keratin biomacromolecular nitric oxide donor. The keratin biomacromolecular nitric oxide donor contains reduced keratin obtained through reduction treatment on keratin, and SNO groups are formed on the sulfydryl groups of the reduced keratin. The keratin biomacromolecular nitric oxide donor is an S-nitrosated keratin and is synthesized and prepared through reaction between the reduced kertin and tert-butyl nitrite or sodium nitrite. The keratin biomacromolecular nitric oxide donor is high in molecular weight, stable in storage and capable of releasing nitric oxide under certain conditions. The keratin biomacromolecular nitric oxide donor can directly serve as a nitric oxide donor as well as be compounded with other polymers to prepare nitric oxide releasing materials to give play to a controllable nitric oxide releasing function, thereby being applicable to preparing materials such as artificial blood vessels, intravascular stent and wound dressings.

Description

technical field [0001] The present invention relates to a nitric oxide donor, in particular to a keratin biomacromolecule nitric oxide donor and a synthesis method thereof, and the nitric oxide donor can be used in biological materials such as blood contact materials and wound healing materials. application in medical materials. Background technique [0002] In 1992, NO became the star molecule of the year of "Science" magazine. The 1998 Nobel Prize in Medicine was awarded to American scientists Ignarro, Murad and Fuchgott in recognition of their outstanding contributions to the study of "NO as a signaling molecule in the cardiovascular system". NO has three main functions in the cardiovascular system: inhibiting platelet aggregation, relaxing blood vessels, and preventing myocardial ischemia-reperfusion injury. In addition, NO can also kill bacteria, reduce inflammation, and promote wound healing. [0003] Nitric oxide donors mainly include organic nitrates, metal-NO com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C07K1/113A61L15/32A61L15/44A61L27/22A61L27/50A61L27/54A61L31/04A61L31/16
CPCA61L15/32A61L15/44A61L27/227A61L27/507A61L27/54A61L31/047A61L31/16A61L2300/252A61L2300/42C07K14/4741
Inventor 袁江汪燕芳沈健
Owner NANJING NORMAL UNIVERSITY
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