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Method for detecting Smilax smilax fumigated by sulfur and its products

A technology of sulfur fumigation and Smilax smilax, which is applied in the field of detection of sulfur fumigated Smilax smilax and its products, can solve the problems that the rapid detection method of sulfur fumigation Smilax smilax has not been reported yet, and achieves accurate and reliable calculation results, good effect, and economical the effect of time

Inactive Publication Date: 2020-08-21
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing reports, there is no research on the changes of the chemical components of Smilax smilax after sulfur fumigation, and there is no report on the rapid detection method of sulfur fumigation Smilax smilax

Method used

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  • Method for detecting Smilax smilax fumigated by sulfur and its products
  • Method for detecting Smilax smilax fumigated by sulfur and its products
  • Method for detecting Smilax smilax fumigated by sulfur and its products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Step 1. Take the fresh Smilax smilax medicinal material and the sulfur-fumigated Smilax smilax medicinal material, slice and dry them separately, and then grind them into powders. Take 0.5g of each and place them in 50ml volumetric flasks, add 20mL of 80% methanol, and use ultrasonic extraction to extract 20- Take it out after 30 minutes, cool to room temperature, add 80% methanol to the volume, shake well, filter, and take the subsequent filtrate as sample solution a and sample solution b.

[0038]Step 2, using ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry to collect ion fragment mass spectrometry data for sample solution a and sample solution b respectively, wherein the chromatographic conditions are: use ACQUITY UPLC HSS T3 chromatographic column ( 2.1mm×100mm, 1.8μm); Gradient elution is adopted, phase A in the mobile phase is 0.1% formic acid aqueous solution, phase B is acetonitrile, and the ratio of acetonitrile is: 5–10%...

Embodiment 2

[0050] On the basis of Example 1, the following steps are added after step 1 and before step 2: add 1.0×10 -4 mol / L catechol PBS buffer solution, the pH value of the selected PBS buffer solution is 7.4, the phosphate concentration is 0.1mol / L, and a three-electrode system with a glassy carbon electrode as the working electrode is used to add The sample solution a and sample solution b with PBS buffer solution were scanned by cyclic voltammetry, and the scanning parameters were set as: initial potential -0.4V, highest potential 1V, lowest point -0.4V, final potential -0.4V, scan rate 0.05V / s, scan times 2 times, sensitivity 10 -4 A / V, waiting time is 2s.

[0051] After the above steps, the time required for ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry is further shortened. At the same time, after the heavy metal ions in the sample solution are quickly separated, some invalid mass spectra that affect the analysis are also eliminated. ...

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Abstract

The invention discloses a method for detecting glabrous greenbrier rhizome or products thereof fumigated by sulfur, and the method comprises the following steps: step 1, preparing a sample solution a of glabrous greenbrier rhizome or products thereof fumigated by sulfur and a sample solution b of glabrous greenbrier rhizome or products thereof not fumigated by sulfur; step 2, respectively carrying out mass spectrometric data acquisition on the sample solution a and the sample solution b by an ultra-performance liquid chromatography-quadrupole-time of flight-mass spectrometry method, wherein the adopted collision voltage is 35-50V; and step 3, contrasting and determining the mass spectrometric data, namely the mass spectrometric data of symbolic vulcanized products or chips thereof, of the differential components emerging in the sample solution a relative to the sample solution b by a multivariate statistical analysis method. The method realizes quick detection of the glabrous greenbrier rhizome and products thereof fumigated by sulfur for the first time, and simultaneously has a simple sample treatment method, short analysis time, strong specificity and accurate detection results.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine detection. More specifically, the present invention relates to a method for detecting sulfur-fumigated Smilax smilax and its products. Background technique [0002] Smilax smilax is the dry rhizome of Smilax glabra, a plant of the genus Smilax in the family Liliaceae. It is a resource for both medicine and food. Modern studies have shown that the eucalyptus has a wide range of pharmacological activities, mainly manifested in cardiovascular pharmacological effects, anti-tumor, anti-inflammatory, immune regulation, anti-oxidation, antibacterial, uric acid lowering, and renal function protection. It is mainly used clinically to treat microbial Infections, diseases of the genitourinary system, connective tissue system, skin diseases, digestive system, etc. Smilax tuckahoe is often used as a food tonic or as a health product among the people, especially in southern China, it is also common...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/34G01N30/06G01N30/14G01N30/86
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/72G01N30/86G01N30/8631G01N30/8641
Inventor 何丽丽张志峰
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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